Summary. Microbial N produced in the rumen and flowing to the duodenum (N i (Egan, 1974). Both, N incorporated into microbial crude protein and leaving the rumen (N i ), and OM f can be estimated in vivo using cannulated animals and markers for the determination of digesta flow and microbial N. Apart from differences in cell composition (Armstrong, 1980), the relationship of N i to OM f reflects the evident limitation of