Microbial gut evaluation in an angolan paediatric population with sickle cell disease

Delgadinho, Mariana; Ginete, Catarina; Santos, Branca Maria de Oliveira; Mendes, Joana; Miranda, Armandina; Vasconcelos, Jocelyne Neto de; Brito, Miguel

doi:10.1111/jcmm.17402

Cited by 14 publications

(13 citation statements)

References 36 publications

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“…31 Rhodospirillales sp., also less abundant in TOW mice, had low abundance in patients with SCD. 29 We found higher abundances of Lactobacillus sp., Monoglobus sp., Odoribacter sp., Lachnoclostridium sp., Muribaculaceae, Clostridia_vadinBB60_group , Oscillibacter sp., and Muribaculum sp. in TOW mice.…”

Section: Discussionmentioning

confidence: 67%

Gut microbiota dysbiosis alters chronic pain behaviors in a humanized transgenic mouse model of sickle cell disease

Kashyap,

Wang

2023

Pain

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Pain is the most common symptom experienced by patients with sickle cell disease (SCD) throughout their lives and is the main cause of hospitalization. Despite the progress that has been made towards understanding the disease pathophysiology, major gaps remain in the knowledge of SCD pain, the transition to chronic pain, and effective pain management. Recent evidence has demonstrated a vital role of gut microbiota in pathophysiological features of SCD. However, the role of gut microbiota in SCD pain is yet to be explored. We sought to evaluate the compositional differences in the gut microbiota of transgenic mice with SCD and nonsickle control mice and investigate the role of gut microbiota in SCD pain by using antibiotic-mediated gut microbiota depletion and fecal material transplantation (FMT). The antibiotic-mediated gut microbiota depletion did not affect evoked pain but significantly attenuated ongoing spontaneous pain in mice with SCD. Fecal material transplantation from mice with SCD to wild-type mice resulted in tactile allodynia (0.95 ± 0.17 g vs 0.08 ± 0.02 g, von Frey test, P < 0.001), heat hyperalgesia (15.10 ± 0.79 seconds vs 8.68 ± 1.17 seconds, radiant heat, P < 0.01), cold allodynia (2.75 ± 0.26 seconds vs 1.68 ± 0.08 seconds, dry ice test, P < 0.01), and anxiety-like behaviors (Elevated Plus Maze Test, Open Field Test). On the contrary, reshaping gut microbiota of mice with SCD with FMT from WT mice resulted in reduced tactile allodynia (0.05 ± 0.01 g vs 0.25 ± 0.03 g, P < 0.001), heat hyperalgesia (5.89 ± 0.67 seconds vs 12.25 ± 0.76 seconds, P < 0.001), and anxiety-like behaviors. These findings provide insights into the relationship between gut microbiota dysbiosis and pain in SCD, highlighting the importance of gut microbial communities that may serve as potential targets for novel pain interventions.

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Section: Discussionmentioning

confidence: 67%

Gut microbiota dysbiosis alters chronic pain behaviors in a humanized transgenic mouse model of sickle cell disease

Kashyap,

Wang

2023

Pain

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“…A reduced nutritional intake is known to shift the gut microbiome. For example, iron deficiency results in a reduction of Bifidob acterium, [210][211][212] Bacteroides, 213 and Faecalibacterium 214 and causes a shift in SCFA 215 as well as siderophore-production, 213 while an increase of the Actinobacteria phylum [216][217][218] is reported. Vitamin A deficiency rather promotes Enterococcus in children with diarrhea, 219 while Bacteroides and Bifidobacterium are decreased.…”

Section: Inflammationmentioning

confidence: 99%

Nutrition in chronic inflammatory conditions: Bypassing the mucosal block for micronutrients

Roth‐Walter,

Berni Canani,

O'Mahony

et al. 2023

Allergy

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Nutritional Immunity is one of the most ancient innate immune responses, during which the body can restrict nutrients availability to pathogens and restricts their uptake by the gut mucosa (mucosal block). Though this can be a beneficial strategy during infection, it also is associated with non‐communicable diseases—where the pathogen is missing; leading to increased morbidity and mortality as micronutritional uptake and distribution in the body is hindered. Here, we discuss the acute immune response in respect to nutrients, the opposing nutritional demands of regulatory and inflammatory cells and particularly focus on some nutrients linked with inflammation such as iron, vitamins A, Bs, C, and other antioxidants. We propose that while the absorption of certain micronutrients is hindered during inflammation, the dietary lymph path remains available. As such, several clinical trials investigated the role of the lymphatic system during protein absorption, following a ketogenic diet and an increased intake of antioxidants, vitamins, and minerals, in reducing inflammation and ameliorating disease.

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“…26 They observed differences at the microbial composition when they modeled the top 15 bacterial genera, but did not find differences at the phylum level or in diversity. Delgadinho and colleagues showed that Angolan children (3-14 years) 40 had higher Actinobacteria phylum, and Clostridium were more prevalent in the SCA population. In our study, the gut microbial diversity was similar between the HbSC (less severe form of sickle cell disease) and HC, but the alpha diversity was different between the SCA (HbSS) cohort and HC, although the difference seen did not achieve statistical significance.…”

Section: Discussionmentioning

confidence: 99%

The relationship between nutrition, gut dysbiosis, and pediatric sickle cell pain outcomes: A pilot study

Dike

Hanson

Davies

et al. 2023

Pediatric Blood & Cancer

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Background Nutritional deficiencies are prevalent in sickle cell disease (SCD) and may be associated with worse pain outcomes. Gut dysbiosis has been reported in patients with SCD and may contribute to both nutritional deficiencies and pain. Objectives We tested the association of nutrition, fat‐soluble vitamin (FSV) deficiency, and gut microbiome composition on clinical outcomes in SCD. Second, we measured the association between diet and exocrine pancreatic function on FSV levels. Methods Using case control design, we enrolled children with SCD (n = 24) and matched healthy controls (HC; n = 17, age, sex, race/ethnicity). Descriptive statistics summarized demographic and clinical data. Wilcoxson‐rank tests compared FSV levels between cohorts. Regression modeling tested the association between FSV levels and SCD status. Welch's t‐test with Satterthwaite adjustment evaluated associations between microbiota profiles, SCD status, and pain outcomes. Results Vitamin A and D levels were significantly decreased in participants with HbSS as compared to HC (vitamin A, p = < .0001, vitamin D, p = .014) independent of nutritional status. FSV correlated with dietary intake in SCD and HC cohorts. Gut microbial diversity was reduced in hemoglobin SS (HbSS) compared to hemoglobin SC (HbSC) and HC, p = .037 and .059, respectively. The phyla Erysipelotrichaceae and Betaproteobacteria were higher in SCD children reporting the highest quality‐of‐life (QoL) scores (p = .008 and .049, respectively), while Clostridia were higher in those with lower QoL scores (p = .03). Conclusion FSV deficiencies and gut dysbiosis are prevalent in children with SCA. Gut microbial composition is significantly different in children with SCD with low QoL scores.

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Microbial gut evaluation in an angolan paediatric population with sickle cell disease

Cited by 14 publications

References 36 publications

Gut microbiota dysbiosis alters chronic pain behaviors in a humanized transgenic mouse model of sickle cell disease

Gut microbiota dysbiosis alters chronic pain behaviors in a humanized transgenic mouse model of sickle cell disease

Nutrition in chronic inflammatory conditions: Bypassing the mucosal block for micronutrients

The relationship between nutrition, gut dysbiosis, and pediatric sickle cell pain outcomes: A pilot study

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