Microbial hazards in plant tissue and cell cultures

The prospect of micropropagating Gonystylus bancanus (Miq.) Kurz, a tropical peat swamp forest timber species through tissue culture technique - Review

“…According to Leifert and Cassells (2001), contaminants in tissue cultures vary in a wide range of microorganisms including filamentous fungi, yeasts, bacteria, viruses, viroids, mites and even thrips. While the fungus may arrive from the explant or airborne, contamination by bacteria was believed to originate from endogenous bacteria that escape initial disinfection or by microorganisms introduced during tissue culture manipulations such as subculture to fresh media.…”

“…It is interesting to note that, even though some experiments may give comparatively highly clean culture when tested using a particular technique, these results are not usually replicable on other tissue culture work and so the expected results are usually elusive (Barrett, Cassells 1994). In many cases anti-microbial treatments only inhibit contaminants and low levels of contamination persist even though some researchers incorporated the use of antibiotics and fungicides to overcome the contamination problem (Leifert, Cassells 2001). Another problem that affects tissue culture work of most tropical tree species is browning.…”

Antibiotics Efficacy on In Vitro Growth Parameters of Important Potato Cultivars

“…Antifungal agents like amphotericin B and antimycotic are one of the polyene antibiotics and is widely used for the treatment of systemic fungal infections. In plants grown in vitro, the presence of microorganisms with the culture medium often is associated to contamination, thus their unwanted presence, since one of the objectives of this technique is precisely to obtain axenic plants, or entirely free microorganisms (Leifert and Cassells, 2001;Harshal et al, 2014). Therefore, a study was conducted to investigate the effect of these antifungal agents (Amphotericin-B and Antimycotic) for different concentrations for four varieties (Kufri Jyoti, Kufri Sadabahar, Kufri Lauvkar and Kufri Sindhuri) to find out contamination free, vigorous in-vitro growth of the microplant in MS media.…”

Antibioticoterapia em microplantas de abacaxizeiro (Ananas comosus)

“…Em plantas cultivadas in vitro, a presença de microrganismos junto ao meio de cultura, muitas vezes, está associada à contaminação, sendo assim sua presença indesejada, já que um dos objetivos desta técnica é justamente a obtenção de plantas axênicas, ou seja, totalmente isentas de microrganismos (LEIFERT & CASSELLS, 2001).…”