Microbial inactivation by high-pressure

Spilimbergo, Sara; Elvassore, Nicola; Bertucco, Alberto

doi:10.1016/s0896-8446(01)00106-1

Cited by 191 publications

(124 citation statements)

References 19 publications

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“…Dissolved CO 2 is thought to penetrate the cells, and the generated H 2 CO 3 dissociates into HCO 3 − and H + , leading to acidification of the cytoplasm (Dixon and Kell, 1989;Dillow et al, 1999;Spilimbergo et al, 2002). Acidification of cytoplasm has been shown to induce injury and affect the viability of vegetative microbial cells (Kim et al, 2008;Wu et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Effects of Carbonation with Heating on Germination of Bacillus subtilis Spores

Noma

Yamashita

Klangpetch

et al. 2011

FSTR

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The effects of carbonation with heating (CH) on germination of Bacillus subtilis spores were investigated. Treatment conditions for CH and heat treatment alone were set to obtain an approximate 1 log reduction in viable count. Pre-treatment of spores with CH at 80℃ and 5 MPa for 30 min significantly decreased their heat resistance to a subsequent heating process at 90℃ for 30 min, as compared with pretreatment by heat alone at 90℃ for 30 min. Treatment with CH also decreased refractility and enhanced DAPI staining when compared with heat treatment alone, thus suggesting that CH effectively initiates and stimulates germination of B. subtilis spores.

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Section: Introductionmentioning

confidence: 99%

Effects of Carbonation with Heating on Germination of Bacillus subtilis Spores

Noma

Yamashita

Klangpetch

et al. 2011

FSTR

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“…As a result of this sterilizing power, subcritical CO 2 and scCO 2 has been used to inactivate bacterial cells and spores when injected into poly(lactic acid) for medical sterilization (17) and for sterilization of food stuffs (20). Even short exposure times (150 sec) to CO 2 at just above its critical point (38°C, 74 bar) are sufficient to inactivate both Gram-negative and Gram-positive bacteria (21).…”

mentioning

confidence: 99%

Mammalian cell survival and processing in supercritical CO ₂

Ginty

Howard²,

Rose³

et al. 2006

Proc. Natl. Acad. Sci. U.S.A.

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We demonstrate that mammalian cells can survive for 5 min within high-pressure CO 2. Cell survival was investigated by exposing a range of mammalian cell types to supercritical CO 2 (scCO2) (35°C, 74 bar; 1 bar ‫؍‬ 100 kPa) for increasing exposure and depressurization times. The myoblastic C2C12 cell line, 3T3 fibroblasts, chondrocytes, and hepatocytes all displayed appreciable but varying metabolic activity with exposure times up to 1 min. With depressurization times of 4 min, cell population metabolic activity was >70% of the control population. Based on survival data, we developed a single-step scCO 2 technique for the rapid production of biodegradable poly(DL-lactic acid) scaffolds containing mammalian cells. By using optimum cell-survival conditions, scCO 2 was used to process poly(DL-lactic acid) containing a cell suspension, and, upon pressure release, a polymer sponge containing viable mammalian cells was formed. Cell functionality was demonstrated by retention of an osteogenic response to bone morphogenetic protein-2 in C2C12 cells. A gene microarray analysis showed no statistically significant changes in gene expression across 4,418 genes by a single-class t test. A significance analysis of microarrays revealed only eight genes that were down-regulated based on a ␦ value of 1.0125 and a false detection rate of 0.biodegradable polymer ͉ bone ͉ scaffolds S tandard methods of combining mammalian cells and synthetic polymers for biotechnological applications (1-3) must minimize disruption to the cell component from fluctuations in solvent composition, temperature, pressure, and shear forces (4, 5). However, processing synthetic polymers by conventional routes requires organic solvents, elevated temperatures or pressures, and sometimes mechanical agitation to produce the required 3D form (6, 7). For this reason, an inefficient two-step process is required to generate the prefabricated polymer structure, with the cell component seeded as a second step (8).One method for generating macroporous, biodegradable scaffolds is by using high-pressure or supercritical CO 2 (scCO 2 ) processing. This technique is solvent-free and has been used for the fabrication of tissue engineering and drug delivery devices because of a number of processing advantages (9, 10). When CO 2 is raised above the critical pressure and temperature (31.2°C, 73.8 bar; 1 bar ϭ 100 kPa), it forms a phase with the characteristics of both a liquid and a gas with selective solvent power and high diffusivity (11). It is then able to penetrate into the amorphous regions of polymers, such as poly(DL-lactic acid) (P DL LA), liquefying them at near ambient temperatures. Release of the pressure results in the nucleation of gas bubbles within the polymer, which become permanent upon solidification, creating a reticulated porous structure (9). ʈ As a result, scaffold fabrication is possible without introducing high temperatures or organic solvents. This fact has made it possible for sensitive biological molecules, such as protein drugs, to be incorporate...

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“…Furthermore, application of SC-CO 2 seems to be attractive for its economical feasibility, as it needs very low pressure (lower than 20 MPa) compared to the so-called ultra high pressure treatment (200-700 MPa) (Bertucco and Vetter, 2001). A number of papers have been addressed to the inactivation of a wide range of microorganisms, bacteria, spores, and yeasts in physiological solutions by SCF Erkmen, 2001;Spilimbergo et al, 2002;CleryBarraud et al, 2004). In the field of industrial applications, it is worthy to quote some recent publications that have dealt with the inactivation in complex substrates and solid food (Haas et al 1989;Gould, 2003;Arreola et al, 1991;Erkmen, 2000;2001).…”

Section: The Inactivation Of Food Related Bacteriamentioning

confidence: 99%

“…milk, fruit juice, and eggs) in a batch system. Spilimbergo has also checked with high pressure CO 2 on red orange juice of Sicily (Spilimbergo et al, 2002). Spores of B. coagulans, B. subtilis, B. cereus, B. licheniformis, and Geobacillus stearothermophilus were subjected to CO 2 treatment at 30-200 MPa and 35-65°C.…”

Section: The Inactivation Of Food Related Bacteriamentioning

confidence: 99%

Supercritical Fluid Application in Food and Bioprocess Technology

Mozafari¹

2011

Mass Transfer - Advanced Aspects

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Microbial inactivation by high-pressure

Cited by 191 publications

References 19 publications

Effects of Carbonation with Heating on Germination of Bacillus subtilis Spores

Effects of Carbonation with Heating on Germination of Bacillus subtilis Spores

Mammalian cell survival and processing in supercritical CO ₂

Supercritical Fluid Application in Food and Bioprocess Technology

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Microbial inactivation by high-pressure

Cited by 191 publications

References 19 publications

Effects of Carbonation with Heating on Germination of Bacillus subtilis Spores

Effects of Carbonation with Heating on Germination of Bacillus subtilis Spores

Mammalian cell survival and processing in supercritical CO 2

Supercritical Fluid Application in Food and Bioprocess Technology

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Product

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Mammalian cell survival and processing in supercritical CO ₂