Microbial Mediated Synthesis of Silver Nanoparticles by Lactobacillus Plantarum TA4 and its Antibacterial and Antioxidant Activity

Rahman, Nor'aini Abdul; Mohamad, Rosfarizan; Zaidan, Uswatun Hasanah

doi:10.3390/app10196973

Cited by 55 publications

(21 citation statements)

References 63 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The evaluation of the AgNPs’ antimicrobial activity was carried out based on the method of Mohd Yusof et al [ 24 ] with slight modification. Agar well-diffusion method against Gram-positive ( Staphylococcus aureus, Staphylococcus epidermidis ) and Gram-negative ( Escherichia coli and Salmonella sp.)…”

Section: Methodsmentioning

confidence: 99%

Interrelations of Synthesis Method, Polyethylene Glycol Coating, Physico-Chemical Characteristics, and Antimicrobial Activity of Silver Nanoparticles

et al. 2020

Self Cite

View full text Add to dashboard Cite

Silver nanoparticles (AgNPs) have been found to have extensive biomedical and biological applications. They can be synthesised using chemical and biological methods, and coated by polymer to enhance their stability. Hence, the changes in the physico-chemical characteristics of AgNPs must be scrutinised due to their importance for biological activity. The UV-Visible absorption spectra of polyethylene glycol (PEG) -coated AgNPs displayed a distinctive narrow peak compared to uncoated AgNPs. In addition, High-Resolution Transmission Electron Microscopy analysis revealed that the shapes of all AgNPs, were predominantly spherical, triangular, and rod-shaped. Fourier-Transform Infrared Spectroscopy analysis further confirmed the role of PEG molecules in the reduction and stabilisation of the AgNPs. Moreover, dynamic light scattering analysis also revealed that the polydispersity index values of PEG-coated AgNPs were lower than the uncoated AgNPs, implying a more uniform size distribution. Furthermore, the uncoated and PEG-coated biologically synthesised AgNPs demonstrated antagonisms activities towards tested pathogenic bacteria, whereas no antagonism activity was detected for the chemically synthesised AgNPs. Overall, generalisation on the interrelations of synthesis methods, PEG coating, characteristics, and antimicrobial activity of AgNPs were established in this study.

show abstract

Section: Methodsmentioning

confidence: 99%

Interrelations of Synthesis Method, Polyethylene Glycol Coating, Physico-Chemical Characteristics, and Antimicrobial Activity of Silver Nanoparticles

et al. 2020

Self Cite

View full text Add to dashboard Cite

show abstract

“…Compared with liquid agents, solid ones have many advantages, such as easy storage and transport, a longer action time, the protection of degrading bacteria from environmental influences, and the provision of a suitable microenvironment for bacterial survival (Ruan et al, 2018;Zhu et al, 2021). There have been some reports on the commercialization of high-efficiency degrading strains for the remediation of soil and water (Sayed and Behle, 2017a;Mohd Yusof et al, 2019). The solid agents prepared by the cyclic aromatic hydrocarbon-degrading bacteria greatly improved the degradation of the organic pollutant pyrene in soil (Wang et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

Development of solid agents of the diphenyl ether herbicide degrading bacterium Bacillus sp. Za based on a mixed organic fertilizer carrier

Zhao

Tian

et al. 2022

Front. Microbiol.

View full text Add to dashboard Cite

The long-term and widespread use of diphenyl ether herbicides has caused serious soil residue problems and threatens the agricultural ecological environment. The development of biodegrading agents using high-efficiency degrading strains as pesticide residue remediation materials has been widely recognized. In this study, the strain Bacillus sp. Za was used to prepare solid agents for the remediation of diphenyl ether herbicides-contaminated soil. The ratio of organic fertilizer was 1:3 (pig manure: cow dung), the inoculum amount of Za was 10%, the application amount of solid agents was 7%, and the application mode was mixed application, all of which were the most suitable conditions for solid agents. After the solid agents were stored for 120 days, the amount of Za remained above 108 CFU/g. The degradation rates of the solid agents for lactofen, bifenox, fluoroglycofen, and fomesafen in soil reached 87.40, 82.40, 78.20, and 65.20%, respectively, on the 7th day. The application of solid agents alleviated the toxic effect of lactofen residues on maize seedlings. A confocal laser scanning microscope (CLSM) was used to observe the colonization of Za-gfp on the surface of maize roots treated in the solid agents, and Za-gfp mainly colonized the elongation zone and the mature area of maize root tips, and the colonization time exceeded 21 days. High-throughput sequencing analysis of soil community structural changes in CK, J (solid agents), Y (lactofen), and JY (solid agents + lactofen) groups showed that the addition of solid agents could restore the bacterial community structure in the rhizosphere soil of maize seedlings. The development of solid agents can facilitate the remediation of soil contaminated with diphenyl ether herbicide residues and improve the technical level of the microbial degradation of pesticide residues.

show abstract

“…This issue does not arise when NPs are synthesized using biological synthesis routes such as by plant extract or microorganisms. Biological synthesis does not require external chemical sources for reducing and stabilizing, as the reactions are mediated by the biological molecules present within the system [6,24]. Moreover, the production of ZnO NPs using biological molecules is of interest for sustainable production and biomedical applications, in line with the green chemistry prospect.…”

Section: Introductionmentioning

confidence: 99%

Antibacterial Potential of Biosynthesized Zinc Oxide Nanoparticles against Poultry-Associated Foodborne Pathogens: An In Vitro Study

Rahman

Mohamad

Zaidan

et al. 2021

Animals

Self Cite

View full text Add to dashboard Cite

Since the emergence of multidrug-resistant bacteria in the poultry industry is currently a serious threat, there is an urgent need to develop a more efficient and alternative antibacterial substance. Zinc oxide nanoparticles (ZnO NPs) have exhibited antibacterial efficacy against a wide range of microorganisms. Although the in vitro antibacterial activity of ZnO NPs has been studied, little is known about the antibacterial mechanisms of ZnO NPs against poultry-associated foodborne pathogens. In the present study, ZnO NPs were successfully synthesized using Lactobacillus plantarum TA4, characterized, and their antibacterial potential against common avian pathogens (Salmonella spp., Escherichia coli, and Staphylococcus aureus) was investigated. Confirmation of ZnO NPs by UV-Visual spectroscopy showed an absorption band center at 360 nm. Morphologically, the synthesized ZnO NPs were oval with an average particle size of 29.7 nm. Based on the dissolution study of Zn2+, ZnO NPs released more ions than their bulk counterparts. Results from the agar well diffusion assay indicated that ZnO NPs effectively inhibited the growth of the three poultry-associated foodborne pathogens. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were assessed using various concentrations of ZnO NPs, which resulted in excellent antibacterial activity as compared to their bulkier counterparts. S. aureus was more susceptible to ZnO NPs compared to the other tested bacteria. Furthermore, the ZnO NPs demonstrated substantial biofilm inhibition and eradication. The formation of reactive oxygen species (ROS) and cellular material leakage was quantified to determine the underlying antibacterial mechanisms, whereas a scanning electron microscope (SEM) was used to examine the morphological changes of tested bacteria treated with ZnO NPs. The findings suggested that ROS-induced oxidative stress caused membrane damage and bacterial cell death. Overall, the results demonstrated that ZnO NPs could be developed as an alternative antibiotic in poultry production and revealed new possibilities in combating pathogenic microorganisms.

show abstract

Microbial Mediated Synthesis of Silver Nanoparticles by Lactobacillus Plantarum TA4 and its Antibacterial and Antioxidant Activity

Cited by 55 publications

References 63 publications

Interrelations of Synthesis Method, Polyethylene Glycol Coating, Physico-Chemical Characteristics, and Antimicrobial Activity of Silver Nanoparticles

Interrelations of Synthesis Method, Polyethylene Glycol Coating, Physico-Chemical Characteristics, and Antimicrobial Activity of Silver Nanoparticles

Development of solid agents of the diphenyl ether herbicide degrading bacterium Bacillus sp. Za based on a mixed organic fertilizer carrier

Antibacterial Potential of Biosynthesized Zinc Oxide Nanoparticles against Poultry-Associated Foodborne Pathogens: An In Vitro Study

Contact Info

Product

Resources

About