Microbial metabolism of short-chain unsaturated hydrocarbons

Hartmans, S.; Bont, J.A.M. de; Harder, W.

doi:10.1111/j.1574-6968.1989.tb03399.x

Cited by 18 publications

(4 citation statements)

References 149 publications

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“…If solid medium was needed, 2% (w/v) agar and 30 µg mL − 1 gentamycin were added. Pre‐cultures for bioreactor cultivations were performed using 10 g L − 1 glucose and minimal salt medium (MSM) with a final composition (per L) of 11.64 g K 2 HPO 4 , 4.89 g NaH 2 PO 4 , 2 g (NH 4 ) 2 SO 4 , 10 mg EDTA, 100 mg MgCl 2 6 H 2 O, 2 mg ZnSO 4 7 H 2 O, 1 mg CaCl 2 2 H 2 O, 5 mg FeSO 4 7 H 2 O, 0.5 mg Na 2 MoO 4 2 H 2 O, 0.2 mg CuSO 4 5 H 2 O, 0.4 mg CoCl 2 6 H 2 O, and 1 mg MnCl 2 2 H 2 O (modified from Hartmans et al (1989)). For bioreactor cultivation, the K 2 HPO 4 /NaH 2 PO 4 concentration was reduced threefold.…”

Section: Methodsmentioning

confidence: 99%

“…A third approach that allows foam-free aeration without modification of the fermentation system is based on a submerged membrane module. An early example of in situ membrane aeration is the "Lehmann-Reaktor" (Vorlop & Lehmann, 1988) and its later application by Heidemann et al (1994). This shake flask features in situ membrane aeration for the cultivation of cell cultures and a magnetic stirrer rotating the membrane.…”

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confidence: 99%

“…MnCl 2 2 H 2 O (modified fromHartmans et al (1989)). For bioreactor cultivation, the K 2 HPO 4 /NaH 2 PO 4 concentration was reduced threefold.…”

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confidence: 99%

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A novel membrane stirrer system enables foam‐free biosurfactant production

Bongartz

Karmainski

Meyer

et al. 2023

Biotech & Bioengineering

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Bioreactors are the operative backbone, for example, for the production of biopharmaceuticals, biomaterials in tissue engineering, and sustainable substitutes for chemicals. Still, the Achilles' heel of bioreactors nowadays is the aeration which is based on intense stirring and gas sparging, yielding inherent drawbacks such as shear stress, foaming, and sterility concerns. We present the synergistic combination of simulations and experiments toward a membrane stirrer for the efficient bubble-free aeration of bioreactors. A digital twin of the bioreactor with an integrated membrane-module stirrer (MemStir) was developed with computational fluid dynamics (CFD) studies addressing the determination of fluid mixing, shear rates, and local oxygen concentration.Usability of the MemStir is shown in a foam-free recombinant production process of biosurfactants (rhamnolipids) from glucose with different strains of Pseudomonas putida KT2440 in a 3-L vessel and benchmarked against a regular aerated process. The MemStir delivered a maximal oxygen transfer rate (OTR max ) of 175 mmol L −1 h −1 in completely foam-free cultivations. With a high space-time yield (STY) of 118 mg RL L −1 h −1 during a fed-batch fermentation, the effectiveness of the novel MemStir is demonstrated. Simulations show the generic value of the MemStir beyond biosurfactant production, for example, for animal cell cultivation.

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Section: Methodsmentioning

confidence: 99%

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confidence: 99%

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A novel membrane stirrer system enables foam‐free biosurfactant production

Bongartz

Karmainski

Meyer

et al. 2023

Biotech & Bioengineering

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“…Stock cultures contained cell suspension of the consortium from the enrichment flask (1 ml) and diesel fuel (2.0% v/v) and were cultivated at pH 7.0 in a mineral medium as described by Hartmans et al (1989). After 48 h, appropriate amounts of cell suspension were used for the inoculation of the final culture, with starting optical density of the final culture set to fit 0.1 (measured at 620 nm).…”

Section: Growth Conditions For Diesel Fuel Biodegradation Testsmentioning

confidence: 99%

Biodegradation of diesel fuel by a microbial consortium in the presence of 1-alkoxymethyl-2-methyl-5-hydroxypyridinium chloride homologues

et al. 2009

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Fast development of ionic liquids as gaining more and more attention valuable chemicals will undoubtedly lead to environmental pollution. New formulations and application of ionic liquids may result in contamination in the presence of hydrophobic compounds, such as petroleum mixtures. We hypothesize that in the presence of diesel fuel low-water-soluble ionic liquids may become more toxic to hydrocarbon-degrading microorganisms. In this study the influence of 1-alkoxymethyl-2-methyl-5-hydroxypyridinium chloride homologues (side-chain length from C(3) to C(18)) on biodegradation of diesel fuel by a bacterial consortium was investigated. Whereas test performed for the consortium cultivated on disodium succinate showed that toxicity of the investigated ionic liquids decreased with increase in side-chain length, only higher homologues (C(8)-C(18)) caused a decrease in diesel fuel biodegradation. As a result of exposure to toxic compounds also modification in cell surface hydrophobicity was observed (MATH). Disulphine blue active substances method was employed to determine partitioning index of ionic liquids between water and diesel fuel phase, which varied from 1.1 to 51% for C(3) and C(18) homologues, respectively. We conclude that in the presence of hydrocarbons acting as a solvent, the increased bioavailability of hydrophobic homologues is responsible for the decrease in biodegradation efficiency of diesel fuel.

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Microbial Detection of Various Pollutants as an Early Warning System for Monitoring of Water Quality and Ecological Integrity of Natural Resources, inRussia

Dimitrieva-Moats

2004

Water Encyclopedia

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The author of this paper had long‐term extensive experience working with water quality control and ecological integrity monitoring using microbial indices. This practice includes eight years of microbial bioassessments and the development of biocriteria implications for the detection of various organic pollutants, a number of heavy metals, and biological contamination in seawaters of Russia, which provided impressive results, demonstrating the advantages of such an approach for monitoring ecological integrity and water quality of estuaries and shallow and pelagic regions of marine coastal zones. The composition alteration of aquatic sediment or plankton microbial assemblages subjected to pollution resulted in an increase of pollutant‐resistant (indicator) groups that detected expected and unexpected pollutants and their levels. The analysis of the proportion between all indicator groups assisted us in drawing conclusions about the natural or anthropogenic process or factors causing impairment of water resources.

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Microbial metabolism of short-chain unsaturated hydrocarbons

Cited by 18 publications

References 149 publications

A novel membrane stirrer system enables foam‐free biosurfactant production

A novel membrane stirrer system enables foam‐free biosurfactant production

Biodegradation of diesel fuel by a microbial consortium in the presence of 1-alkoxymethyl-2-methyl-5-hydroxypyridinium chloride homologues

Microbial Detection of Various Pollutants as an Early Warning System for Monitoring of Water Quality and Ecological Integrity of Natural Resources, inRussia

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