Microbial methane oxidation in the River Saar

Zaiss, Ulrich; Winter, Philipp; Kaltwasser, Heinrich

doi:10.1002/jobm.19820220210

Cited by 7 publications

(2 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…). This result is consistent with the detection of such methanotrophs in other freshwater ecosystems (Zaiss et al ; Sundh et al ; Oswald et al ; Samad and Bertilsson ) and previous incubation studies with these microorganisms that indicated growth at cold temperatures (Sundh et al ; Ricão Canelhas et al ). Moreover, these lineages were detected in higher proportions on the large‐pore–size filters than on the small‐pore–size filters (up to 14.89% and 11.84% on the large‐pore–size filters for Crenothrix and Methylobacter, respectively, vs. up to 1.04% and 3.94% on the small‐pore–size filters).…”

Section: Resultssupporting

confidence: 92%

Annual bacterial community cycle in a seasonally ice‐covered river reflects environmental and climatic conditions

Cruaud

Vigneron

Fradette

et al. 2019

Limnology & Oceanography

View full text Add to dashboard Cite

Bacteria are key players in biogeochemical cycles and control water quality in freshwater ecosystems. Nevertheless, little is known about the identity and ecology of riverine bacteria, especially during ice‐covered periods that are often mistakenly perceived as periods with negligible biological activities. Here, we analyzed in detail the effects of environmental and climatic conditions on freshwater bacterial community structure and diversity over a 2‐yr sampling campaign, targeting a seasonally ice‐covered river of the Quebec City (Canada) area, the Saint‐Charles River. Quantitative polymerase chain reaction and 16S ribosomal RNA gene high‐throughput sequencing demonstrated a strong seasonal cycle of the bacterial community composition with rapid successions of bacterial lineages, reflecting the harsh climatic condition of the region. During the summer, the bacterial community was dominated by typical freshwater microorganisms such as Limnohabitans, Sporichthyaceae hgcI/acI clade, and Pseudarcicella. In contrast, the results suggest that during the cold season, the low water temperatures, combined with other prevailing conditions such as reduced light availability and minimal particulate inputs from the catchment, created various environmental niches for potential methanotrophic Gammaproteobacteria, such as Crenothrix and Methylobacter, other Betaproteobacteria, such as Candidatus Methylopumilus, Candidatus Nitrotoga, and Rhodoferax, as well as Verrucomicrobia and Parcubacteria. The presence of these taxa in the winter suggests active carbon, iron, and nitrogen cycling under ice, whereas summer lineages are dormant or in a phase of reduced activity. These results increase our understanding of bacterial dynamic and potential metabolic processes occurring in seasonally ice‐covered inland waters, providing evidence that winter can be an especially important period for freshwater ecological processes.

show abstract

Section: Resultssupporting

confidence: 92%

Annual bacterial community cycle in a seasonally ice‐covered river reflects environmental and climatic conditions

Cruaud

Vigneron

Fradette

et al. 2019

Limnology & Oceanography

View full text Add to dashboard Cite

show abstract

“…MOB constitute the main microbial processors of CH 4 at oxic/anoxic interfaces in stream sediments (Trimmer et al, 2010(Trimmer et al, , 2015Buriánková et al, 2012;Shelley et al, 2014Shelley et al, , 2015 where MOB can account for up to 30% of prokaryotic cell numbers (Buriánková et al, 2012). CH 4 oxidation (MOX) can be an important ecosystem process, equivalent to up to 46% of net photosynthetic production in chalk streams (Trimmer et al, 2010;Shelley et al, 2014) and reducing CH 4 evasion fluxes by 70% (Zaiss et al, 1981;de Angelis and Scranton, 1993). Among MOB, low-affinity CH 4 oxidizers with substrate affinity constants K S [i.e., the substrate concentration for which the MOX rate is half the maximum reaction velocity (V max )] in the micromolar range are adapted to elevated CH 4 concentrations typical for hot spots of methanogenesis.…”

Section: Introductionmentioning

confidence: 99%

Microbial Ecology of Methanotrophy in Streams Along a Gradient of CH4 Availability

et al. 2020

View full text Add to dashboard Cite

Despite the recognition of streams and rivers as sources of methane (CH 4) to the atmosphere, the role of CH 4 oxidation (MOX) in these ecosystems remains poorly understood to date. Here, we measured the kinetics of MOX in stream sediments of 14 sites to resolve the ecophysiology of CH 4 oxidizing bacteria (MOB) communities. The streams cover a gradient of land cover and associated physicochemical parameter and differed in stream-and porewater CH 4 concentrations. Michealis-Menten kinetic parameter of MOX, maximum reaction velocity (V max), and CH 4 concentration at half V max (K S) increased with CH 4 supply. K S values in the micromolar range matched the CH 4 concentrations measured in shallow stream sediments and indicate that MOX is mostly driven by low-affinity MOB. 16S rRNA gene sequencing identified MOB classified as Methylococcaceae and particularly Crenothrix. Their relative abundance correlated with pmoA gene counts and MOX rates, underscoring their pivotal role as CH 4 oxidizers in stream sediments. Building on the concept of enterotypes, we identify two distinct groups of co-occurring MOB. While there was no taxonomic difference among the members of each cluster, one cluster contained abundant and common MOB, whereas the other cluster contained rare operational taxonomic units (OTUs) specific to a subset of streams. These integrated analyses of changes in MOB community structure, gene abundance, and the corresponding ecosystem process contribute to a better understanding of the distal controls on MOX in streams.

show abstract

Verbreitung methanotropher Bakterien

Heyer

Malashenko²,

Berger

et al. 1984

J of Basic Microbiology

View full text Add to dashboard Cite

JIolekularbiologie und Dledixiii. %ent.ralinstitut fur Mikrobiologie und expcrimc!nt,elle Therapie, Jena; 21nst.itut fur Mikrobiologie und Virologie der Akttdemie der Wissenachaftcn der TJSSR, Kiew) Verbreitung rnethanotropher Bakterien tJ. HI<; Y ER1, Y U . MArJASIl13:NKo2, u. BERG1(:lrl wid E. BUUKoVA' (Einycganye'rL u r a 85. d . 1984)This paper reports on blie investig:ht,ions of the distribut,ion of the methnnot,rophic bact,eri;i in various environmentjs and geographic zones. In t,he most of ilbout 1000 samples investigatod by IIS ohligately methanotrophic bacteria were found. Morc: t h i n 300 pure cultures wero isolated, ;~mong t,ticm strains of iIII five groups of methanotrophs dcscvribed by WHITT1':NBIJILY et d. (1970). Thcrc were great differcnccs in their distribution p5tterns i u severa.1 habit,iits. Thc occurrence of met,lia.riolmphic bacteria even in acidophilic peat bog It~kes and in salt-lakes shows t,he great cdogical amplitude of this group of organisms.Experiences with the use and the interpretation of t,he MPN-method for the enumeration of methttnutrophic bacteria are presented. The results of tho quantitative investigat,ions cnltzrgc t,he knowlcdge about the distribution of these organisms in vnrious nltturd and artific;i,l habitats ;~n d confirm t,heir high frequency in the nature.The greatest densities of cells are found in sewage, but only small numbers of cells in tlic opcvi water itrid sandy sediments of Raltic Sea and in salt-lakes. Generally the frequency of the inethanotrophic bacteria wiis closely related to the concentration of mrt,hanc, respectively, to the rate of rnet.ha.nc release in t.he habitat. The high numbers of cells i n the aerobic layers of sediments ikrc ;in indication of the gretbt act'ivity of the aerobic methane OxidiLtion in these zones.

show abstract

Microbial methane oxidation in the River Saar

Cited by 7 publications

References 11 publications

Annual bacterial community cycle in a seasonally ice‐covered river reflects environmental and climatic conditions

Annual bacterial community cycle in a seasonally ice‐covered river reflects environmental and climatic conditions

Microbial Ecology of Methanotrophy in Streams Along a Gradient of CH4 Availability

Verbreitung methanotropher Bakterien

Contact Info

Product

Resources

About