Microbial occurrence and antibiotic resistance in ready-to-go food items

Abstract

Foodborne pathogens, such as and, are commonly prevalent in contaminated food products seen through annual food recalls. Excessive use of antibiotics through the past few decades has led to a multitude of antibiotic resistant bacteria, including foodborne pathogens. We investigated microbial occurrence and their antibiotics resistances in ready-to-go food items, i.e. canned food, bagged food, and baby food. A total of 112 isolates were isolated from varying food items, and 21 of these isolates were identified through 16S rRNA sequencing revealing sp., sp. and sp. Bagged food items showed the most microbial diversity as well as the largest colony forming unit (log 20-25 CFU/g). Isolates showed antibiotic resistance to ampicillin, streptomycin, chloramphenicol, and kanamycin at concentrations of 100, 500, and 1000 µg/mL. 57% isolates were ampicillin resistance followed by kanamycin (26%). A variety of microorganisms present in ready-to-go food items may not be pathogenic, however their occurrence and multiple antibiotic resistance (MAR) poses risk of transferring their genes to foodborne pathogens.

Probiotics: If It Does Not Help It Does Not Do Any Harm. Really?

“…It is very logical that one of the concerns of their massive usage is the lateral exchange of hostile genes, in-between them, or to the physiological microbiome, to the dysbiota, or even to human cells, as summarized here [8]. Gene acquisition/loss within or between various microbes and probiotic strains were widely described, all across the food chain, be it dairy, meat, or vegetable products and even in the ready-to-go food items [121,130,131,132,133,134]. HGT of antibiotic resistance is wide and was reported for a wide range of probiotics, including Lactobacillus rhamnosus , Lactobacillus gasseri , Lactobacillus paracasei , Lactobacillus reuteri , Lactobacillus plantarum, and many others [8].…”

How Has the Hazard to Humans of Microorganisms Found in Atmospheric Aerosol in the South of Western Siberia Changed over 10 Years?

“…In the collections of bacteria isolates collected over a long period of time, the latest trend can be traced reliably [97,98]. Moreover, more and more isolates with multiple antibiotic resistances are detected in nature [99][100][101][102].…”

Extended‐spectrum β‐lactamase‐producing Enterobacteriaceae from ready‐to‐eat foods: Genetic diversity and antibiotic susceptibility

“…As these are consumed without further processing, maintaining hygiene during all food‐processing operations is essential to ensure public health. Nevertheless, microbiological hazards pose challenges to the RTE food industry (Cole & Singh, 2018 ; Iseppi et al, 2018 ; Thirupathi & Krishnan, 2020 ), and hence, it is necessary to study antibiotic‐resistant bacterial strains present in RTE foods.…”

“…to the RTE food industry (Cole & Singh, 2018;Iseppi et al, 2018;Thirupathi & Krishnan, 2020), and hence, it is necessary to study antibiotic-resistant bacterial strains present in RTE foods.…”