Microbial oxidation of alpha-pinene by Serratia marcescens

Wright, S. J. L.; Caunt, P.; Carter, David; Baker, Peter B.

doi:10.1007/bf00261919

Cited by 37 publications

(28 citation statements)

References 7 publications

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“…Actinobacteria occur in associations with both pine phloem (Adams et al, 2008;Hulcr et al, 2011) and bark beetles (Cardoza et al, 2006Scott et al, 2008), so some tolerance to monoterpenes would seem necessary for their survival. Some bacteria in the Bacillales are capable of degrading various monoterpenes (Chang and Oriel, 1994;Savithiry et al, 1998) and similar hydrocarbons synthesized by plants (Wright et al, 1986;Jiménez et al, 2010). Unlike the isolates from D. ponderosae, three of the isolates from D. valens were γ-Proteobacteria.…”

Section: Discussionmentioning

confidence: 95%

Responses of Bark Beetle-Associated Bacteria to Host Monoterpenes and Their Relationship to Insect Life Histories

et al. 2011

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Bark beetles that colonize living conifers and their microbial associates encounter constitutive and induced chemical defenses of their host. Monoterpene hydrocarbons comprise a major component of these allelochemicals, and many are antibiotic to insects, fungi, and bacteria. Some bark beetle species exhaust these defenses by killing their host through mass attacks mediated by aggregation pheromones. Others lack adult aggregation pheromones and do not engage in pheromone-mediated mass attacks, but rather have the ability to complete development within live hosts. In the former species, the larvae develop in tissue largely depleted of host terpenes, whereas in the latter exposure to these compounds persists throughout development. A substantial literature exists on how monoterpenes affect bark beetles and their associated fungi, but little is known of how they affect bacteria, which in turn can influence beetle performance in various manners. We tested several bacteria from two bark beetle species for their ability to grow in the presence of a diversity of host monoterpenes. Bacteria were isolated from the mountain pine beetle, Dendroctonus ponderosae Hopkins, which typically kills trees during colonization, and the red turpentine beetle, Dendroctonus valens LeConte, which often lives in their host without causing mortality. Bacteria from D. ponderosae were gram-positive Actinobacteria and Bacilli; one yeast also was tested. Bacteria from D. valens were Actinobacteria, Bacilli, and γ-Proteobacteria. Bacteria from D. valens were more tolerant of monoterpenes than were those from D. ponderosae. Bacteria from D. ponderosae did not grow in the presence of α-pinene and 3-carene, and grew in, but were inhibited by, β-pinene and β-phellandrene. Limonene and myrcene had little inhibitory effect on bacteria from either beetle species. Tolerance to these antibiotic compounds appears to have resulted from adaptation to living in a terpene-rich environment.

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Section: Discussionmentioning

confidence: 95%

Responses of Bark Beetle-Associated Bacteria to Host Monoterpenes and Their Relationship to Insect Life Histories

et al. 2011

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“…Transformation of -pinene to verbenols by microorganisms (fungi or bacteria) has been reported in the literature, [5][6][7][8][9][10][11] but not many examples exist of the use of plant cells for the conversion of -pinene. 3,12,13) The main transformation products have proved similar regardless of the plant cell or microorganism used for the different studies.…”

Section: Resultsmentioning

confidence: 99%

Formation oftrans-Verbenol and Verbenone from α-Pinene Catalysed by ImmobilisedPicea abiesCells

Vaněk

Halík

Vankova

et al. 2005

Bioscience, Biotechnology, and Biochemistry

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Both enantiomers and the racemate of -pinene were transformed by Picea abies cells immobilised on alginate. The main products were cis-and trans-verbenol, the later being further transformed to verbenone. The enantiomeric purity of each product more or less corresponded to that of the substrate. Transformation by free cells was faster than that by the immobilised cells. The ratio of products differed to some extent between the transformation by free and immobilised cells.

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“…Busmann and Berger [30] described verbenone and trans-verbenol, together with myrtenol and transpinocarveol, as major biotransformation products of α-pinene formed by basidiomycetes fungi. Wright et al [31] used a strain of the bacterium Serratia marcescens, isolated from sewage sludge, which can oxidize the terpene hydrocarbon α-pinene to produce trans-verbenol as the major product, with verbenone and trans-sobrerol as minor products.…”

Section: Pre-screening Experimentsmentioning

confidence: 99%

“…The literature points out that a common problem in the monoterpenes biotransformation is the toxicity of these compounds for the microorganisms [18][19][20][21][22][23][24][25][26][27][28][29][30][31][32][33]. To reduce this effect, the method of cell induction by the substrate has been employed, allowing the microorganism adaptation with lower amounts of substrate [33,34].…”

Section: Kinetic Studymentioning

confidence: 99%

Microbial Oxidation of (-)-α-pinene to Verbenol Production by Newly Isolated Strains

Rottava¹,

Cortina

Zanella

et al. 2010

Appl Biochem Biotechnol

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Verbenol is a bicyclicbicycle secondary allylic alcohol, with pronounced camphor and mint flavor notes, mainly used as food flavoring. This compound is also used to control harmful insects, and hence has potential for using in agriculture, and is an intermediate in the synthesis of valuable perfume and medicinal substances. This work is focused on the microbial oxidation of (-)-α-pinene to verbenol production. To carry out the present study, 405 microorganisms were tested for their ability to bioconvert the substrate. From the isolated microorganisms, 193 were selected in the pre-screening using mineral medium for limonene degradation. At the screening step, 31 strains were able to convert (-)-α-pinene in verbenol. The highest concentration in verbenol from (-)-α-pinene was about 125.6 mg/L for yeast isolated from orange juice industrial residue.

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Microbial oxidation of alpha-pinene by Serratia marcescens

Cited by 37 publications

References 7 publications

Responses of Bark Beetle-Associated Bacteria to Host Monoterpenes and Their Relationship to Insect Life Histories

Responses of Bark Beetle-Associated Bacteria to Host Monoterpenes and Their Relationship to Insect Life Histories

Formation oftrans-Verbenol and Verbenone from α-Pinene Catalysed by ImmobilisedPicea abiesCells

Microbial Oxidation of (-)-α-pinene to Verbenol Production by Newly Isolated Strains

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