Microbial producers of butanol

Березина, О. В.; Zakharova, Natalia V.; Yarotsky, C. V.; Zverlov, Vladimir V.

doi:10.1134/s0003683812070022

Cited by 34 publications

(29 citation statements)

References 93 publications

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“…98 Strain degeneration is a result of genetic changes and is different from loss of ability to produce solvents under unfavorable cultivation conditions. Degeneration is also different from the loss of the solvent production ability due to a mega-plasmid loss which is a common phenomenon for C. acetobutylicum.…”

Section: Strain Degeneration In Solventogenic Clostridiamentioning

confidence: 99%

Biobutanol: the outlook of an academic and industrialist

et al. 2013

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The gradual shift of transportation fuels from oil based fuels to the alternative fuel resources and worldwide demand for energy has been the impetus for research to produce alcohol biofuels from renewable resources. Current bioethanol and biodiesel can, however, not cover an increasing demand for biofuels. Hence, there is an extensive need for advanced biofuels with superior fuel properties. The present review is focused on the developments of biobutanol, which is regarded to be superior to bioethanol in terms of energy density and hygroscopicity. Although acetone-butanolethanol (ABE) fermentation is one of the oldest large-scale fermentation processes, butanol yield by anaerobic fermentation remains sub-optimal. For sustainable industrial scale butanol production, a number of obstacles need to be addressed including choice of feedstock, low product yield, product toxicity to production strain, multiple end-products and downstream processing of alcohol mixtures.Metabolic engineering provides a means for fermentation improvements. Different strategies are employed in the metabolic engineering of Clostridia that aim to enhance the solvent production, improve selectivity for butanol production, and increase the tolerance of Clostridia to solvents. The introduction and expression of a non-clostridial butanol producing pathway in E. coli is most promising strategy for butanol biosynthesis. Several rigorous kinetic and physiological models for fermentation have been formulated, which form useful tool for optimization of the process. Due to the lower butanol titers in the fermentation broth, simultaneous fermentation and product removal techniques have been developed to improve production economics. With the use of new strains, inexpensive substrates, and superior reactor designs, economic ABE fermentation may further attract an attention of researchers all over the world. The present review is attempting to provide an overall outlook on discoveries and strategies that are being developed for commercial n-butanol production.

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Section: Strain Degeneration In Solventogenic Clostridiamentioning

confidence: 99%

Biobutanol: the outlook of an academic and industrialist

et al. 2013

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“…48,54,125,[171][172][173] Currently, butanol is utilized for solvent extraction of fats, dye, nitro enamel, plastificator, butyl acetate, phenol formaldehyde resin, and oil-additive manufacturing. 174 With these benefits, a sequential coculture approach with C. acetobutylicum and C. thermocellum grown on solka floc or a combination of solka floc and aspen wood xylan was implemented. The results indicated an efficient utilization of all hydrolysis products derived, which produced a 1.7-to 2.6-fold increase in total fermentation products.…”

Section: Microbial Consortiamentioning

confidence: 99%

Consolidated bioprocessing for biofuel production: recent advances

Mbaneme-Smith¹,

Chinn²

2015

EECT

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Consolidated bioprocessing (CBP), the combination of saccharolytic enzyme production and secretion, hydrolysis of polysaccharides, and fermentation of available sugars within a single-unit operation, improves cellulose conversion efficiency and decreases lignocellulosic biomass processing costs for producing biofuels and value-added products. Clostridium thermocellum, an anaerobic, thermophilic bacterium is a significant biocatalyst aspirant for CBP, due to its native cellulolytic and ethanologenic capabilities. This review highlights strain development/modification, metabolic engineering, and process improvements associated with CBP in the context of using C. thermocellum as a model biocatalyst to reduce operating expenditures and inhibitory effects for enhanced biofuels production. In addition, opportunities in using a microbial consortia and biofilms are discussed. As an overview of recent advances in CBP technologies to convert lignocellulosic biomass to biofuels, this review gives researchers a platform for future development of efficient and sustainable CBP approaches.

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“…In light of inherent shortcomings associated with ethanol, however, which include its high water solubility and low energy density (attributes that collectively diminish its compatibility with conventional engines and fuel distribution infrastructure) [1], interest continues to shift to the alternative fermentative production of higher (i.e., >2-carbon) alcohol biofuels [2,3], including n-butanol [4][5][6]. With more similar physical and thermodynamic properties, n-butanol represents a potential 'drop in' compatible gasoline replacement [7]. Naturally synthesized by many Clostridium sp.…”

Section: Introductionmentioning

confidence: 99%

Improving n-butanol production in batch and semi-continuous processes through integrated product recovery

Staggs

Nielsen

2015

Process Biochemistry

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Although it represents a promising biofuel, n-butanol production by conventional batch fermentation is limited as a result of its cytotoxic effects. To address this limitation and facilitate semi-continuous fermentation, in situ n-butanol removal has proven to be an effective approach. Exploiting the phenomena of solvent extraction, adsorption, or vaporization, numerous integrated bioprocess configurations have been developed to facilitate selective n-butanol recovery. The objective of this review is to provide a broad overview of different technology options and process configurations to this end, highlighting notable achievements and recent developments. In each case, relevant design considerations critical for improving key production metrics will be discussed, with particular emphasis given to studies that, as a result of relieved product toxicity, have successfully demonstrated further enhanced n-butanol production through semi-continuous operation.

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Microbial producers of butanol

Cited by 34 publications

References 93 publications

Biobutanol: the outlook of an academic and industrialist

Biobutanol: the outlook of an academic and industrialist

Consolidated bioprocessing for biofuel production: recent advances

Improving n-butanol production in batch and semi-continuous processes through integrated product recovery

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