Microbial production of fructosyltransferases for synthesis of pre-biotics

Maiorano, Alfredo Eduardo; Piccoli, Rosane A. M.; Silva, Elda Sabino da; Rodrigues, Maria Filomena de Andrade

doi:10.1007/s10529-008-9793-3

Cited by 126 publications

(78 citation statements)

References 61 publications

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“…Fungal FTases have molecular masses ranging between 180,000 and 600,000 and are homopolymers with two to six monomer units. Some studies stated the optimum temperature and pH for these enzyme activities between 50 and 60°C and from 4.5 to 6.5, respectively (Madlová et al 2000;Maiorano et al 2008).…”

Section: Fos Microbial Production-transfructosylation Enzymesmentioning

confidence: 99%

An Overview of the Recent Developments on Fructooligosaccharide Production and Applications

Dominguez

Rodrigues

Lima

et al. 2013

Food Bioprocess Technol

140

108

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Over the past years, many researchers have suggested that deficiencies in the diet can lead to disease states and that some diseases can be avoided through an adequate intake of relevant dietary components. Recently, a great interest in dietary modulation of the human gut has been registered. Prebiotics, such as fructooligosaccharides (FOS), play a key role in the improvement of gut microbiota balance and in individual health. FOS are generally used as components of functional foods, are generally regarded as safe (generally recognized as safe status-from the Food and Drug Administration, USA), and worth about 150€ per kilogram. Due to their nutrition-and health-relevant properties, such as moderate sweetness, low carcinogenicity, low calorimetric value, and low glycemic index, FOS have been increasingly used by the food industry. Conventionally, FOS are produced through a two-stage process that requires an enzyme production and purification step in order to proceed with the chemical reaction itself. Several studies have been conducted on the production of FOS, aiming its optimization toward the development of more efficient production processes and their potential as food ingredients. The improvement of FOS yield and productivity can be achieved by the use of different fermentative methods and different microbial sources of FOSproducing enzymes and the optimization of nutritional and culture parameter; therefore, this review focuses on the latest progresses in FOS research such as its production, functional properties, and market data.

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Section: Fos Microbial Production-transfructosylation Enzymesmentioning

confidence: 99%

An Overview of the Recent Developments on Fructooligosaccharide Production and Applications

Dominguez

Rodrigues

Lima

et al. 2013

Food Bioprocess Technol

140

108

View full text Add to dashboard Cite

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“…2.4.1.9), or β-fructofuranosidases (FFase, E.C. 3.2.1.26) (Maiorano et al, 2008). FTase catalyzes the transfer of a fructosyl group to a molecule of sucrose or a fructooligosaccharide when a FOS chain which has one fructosyl unit more, is formed.…”

Section: Introductionmentioning

confidence: 99%

“…A number of fructosyltransferic microorganisms have been reported, including fungi belonging to genera Aspergillus (Aspergillus niger, Aspergillus oryzae, Aspergillus foetidus, Aspergillus phoenicis); Fusarium (Fusarium oxysporum); Penicillium (Penicillium citrinum, Penicillium rugulosum, Penicillium purpurogenum) and Aureobasidium (Aureobasidium pullulans) (Sangeetha et al, 2004;Maiorano et al, 2008;Ottoni et al, 2012). Investigations of the effect of the cultivation media composition and cultivation conditions on the FTase production by different strains have been performed by several authors (Vandáková et al, 2004;Lim et al, 2006;Wang and Zhou, 2006).…”

Section: Introductionmentioning

confidence: 99%

Optimization of Medium Composition and Cultivation Parameters for Fructosyltransferase Production by Penicillium aurantiogriseum AUMC 5605

Farid

Kamel

Elsayed

et al. 2015

JABC

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Fructooligosaccharides have been mainly produced by microbial fructosyltransferases (FTase) enzymes. The present work focuses on the optimization of medium composition and cultivation parameters affecting FTase produced by Penicillium aurantiogriseum AUMC 5605 in shake flask cultivation. FTase production was optimized in two steps using DeMeo's fractional factorial design. A 1.46-fold increase in FTase production (105.4 U/mL) was achieved using the optimized culture medium consisting of (g/L): sucrose, 600; yeast extract, 10; K 2 HPO 4 , 5; MgSO 4 ·7H 2 O, 0.5; (NH 4 ) 2 SO 4 , 1.0 and KCl, 0.5. The obtained results showed that the maximum FTase enzyme activity was produced at initial cultivation pH values ranging from 6.0-6.5, at agitation speed of 200 rpm and using vegetative fungal cells as inoculum. Moreover, results showed that optimization of medium composition and some cultivation parameters resulted in an increase of about 93.7% in the enzyme activity than the nonoptimized cultivation conditions after 96 h of cultivation. Additionally, maximum production and specific production rates recorded 2340 U/L/h and 102 U/L/h/g cells, respectively.

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“…FOS also uses as a prebiotic compound, they stimulate the growth of bifidobacteria and have also been used in the prevention of colon cancer [1][2][3]. Therapeutically FOS also uses to reduce cholesterol, phospholipid and triglyceride levels in serum [4,5]. These are also used as a protecting agents in swine from Escherichia coli infections and controlling swine odor.…”

Section: Introductionmentioning

confidence: 99%

“…FOS are found in small amounts in vegetables such as onion, garlic, Jerusalem artichokes, asparagus, bananas, rye, wheat and tomatoes. However, they are produced commercially through the enzymatic synthesis from sucrose by using microbial enzymes having FTase activity [1,2,4,5,7].…”

Section: Introductionmentioning

confidence: 99%

Intensification of Fructosyltransferases and Fructo-Oligosaccharides Production in Solid State Fermentation by Aspergillus awamori GHRTS

Sathish

Prakasham

2013

Indian J Microbiol

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The present work was aimed to investigate the impact of the solid substrates mixture on Fructosyltransferases (FTase) and Fructo-oligosaccharides (FOS) production. An augmented simplex lattice design was used to optimize a three component mixture for FTase production. Among selected substrates corn cobs has highest impact on FTase production followed by wheat bran and rice bran. All two substrates and three substrate combinations showed the highest enzyme production than their individual levels. Among the tested various models quadratic model was found to be the best suitable model to explain mixture design. Corncobs, wheat bran and rice bran in a ratio of approximately 45:29:26 is best suitable for the FTase production by isolated Aspergillus awamori GHRTS. This study signifies mixture design could be effective utilize for selection of best combination of multi substrate for improved production of high value products under solid state fermentation.

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Microbial production of fructosyltransferases for synthesis of pre-biotics

Cited by 126 publications

References 61 publications

An Overview of the Recent Developments on Fructooligosaccharide Production and Applications

An Overview of the Recent Developments on Fructooligosaccharide Production and Applications

Optimization of Medium Composition and Cultivation Parameters for Fructosyltransferase Production by Penicillium aurantiogriseum AUMC 5605

Intensification of Fructosyltransferases and Fructo-Oligosaccharides Production in Solid State Fermentation by Aspergillus awamori GHRTS

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