The present investigation attempted to evaluate the existence and survival of spoilage
microorganisms in four common sea fishes (Poma, Rupchanda, Koral and Tuna) available
in Bangladesh and to determine the effects of cooking temperature to optimize the growth
of fish microflora. Moreover, the status of fish in frozen condition after cooking was also
studied. A total 4 categories of sea fishes were collected from the local shops in Dhaka
city. Raw, cooked and frozen fish samples were analyzed for the existence of pathogenic
bacteria through the conventional cultural techniques and the confirmative biochemical
identification procedures. Total viable bacteria were present in all four fish samples in
raw, cooked and frozen condition up to 6 log CFU/mL. Most of the raw fish samples were
found to harbor a huge population of microorganisms up to 5 log CFU/mL including the
fecal coliforms. Several specific bacterial species like E. coli, Klebsiella spp., Salmonella
spp., Shigella spp., Staphylococcus spp., Pseudomonas spp. and Vibrio spp. were present
in raw samples. However, the microbial load reduced from the fish after cooking and the
status was static in frozen condition. Thus, the incidence of fecal coliforms in raw fish
may be considered as a serious threat to the public health upon consumption of such
fishes.