Microbial repellence properties of engineered spider silk coatings prevent biofilm formation of opportunistic bacterial strains

Sommer, Christoph; Bargel, Hendrik; Raßmann, Nadine; Scheibel, Thomas

doi:10.1557/s43579-021-00034-y

Cited by 7 publications

(3 citation statements)

References 51 publications

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“…It is a low-virulence opportunistic pathogen, which may cause a wide range of infections, often following surgery and in relation with the use of medical devices [ 88 ]. Cases of elbow joint and prosthetic joint infections, as well as post-operative discitis, have also been reported [ 89 , 90 , 91 , 92 ]. Corynebacterium vitaeruminis has already been proved to be safe and non-pathogenic; indeed, this strain was negative for 50 tested virulence and resistance genes based on performed PCR, according to Colombo et al [ 93 ].…”

Section: Resultsmentioning

confidence: 99%

Compositional Data Analysis of 16S rRNA Gene Sequencing Results from Hospital Airborne Microbiome Samples

Perrone

Romano

Maria

et al. 2022

IJERPH

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The compositional analysis of 16S rRNA gene sequencing datasets is applied to characterize the bacterial structure of airborne samples collected in different locations of a hospital infection disease department hosting COVID-19 patients, as well as to investigate the relationships among bacterial taxa at the genus and species level. The exploration of the centered log-ratio transformed data by the principal component analysis via the singular value decomposition has shown that the collected samples segregated with an observable separation depending on the monitoring location. More specifically, two main sample clusters were identified with regards to bacterial genera (species), consisting of samples mostly collected in rooms with and without COVID-19 patients, respectively. Human pathogenic genera (species) associated with nosocomial infections were mostly found in samples from areas hosting patients, while non-pathogenic genera (species) mainly isolated from soil were detected in the other samples. Propionibacterium acnes, Staphylococcus pettenkoferi, Corynebacterium tuberculostearicum, and jeikeium were the main pathogenic species detected in COVID-19 patients’ rooms. Samples from these locations were on average characterized by smaller richness/evenness and diversity than the other ones, both at the genus and species level. Finally, the ρ metrics revealed that pairwise positive associations occurred either between pathogenic or non-pathogenic taxa.

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Section: Resultsmentioning

confidence: 99%

Compositional Data Analysis of 16S rRNA Gene Sequencing Results from Hospital Airborne Microbiome Samples

Perrone

Romano

Maria

et al. 2022

IJERPH

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“…Furthermore, the recombinant spider silk protein coatings acted as a bioshield, improved the biocompatibility and significantly decreased the foreign body response to the implant [201,202]. Additionally, it was shown that coatings made of eADF4(C16) silk protein on silicone surfaces could also enhance microbe-repellent properties against four opportunistic infection-related strains, leading to reduced microbial adherence up to 99.7% in comparison to uncoated silicone surfaces [203]. Zhang et al reported a different repellent effect, due to triboelectric charging of a film made from recombinant spider silk proteins based on a MaSp1 sequence [204].…”

Section: Antiadhesive Propertiesmentioning

confidence: 99%

Bioselectivity of silk protein-based materials and their bio-inspired applications

Bargel¹,

Trossmann²,

Sommer³

et al. 2022

Beilstein J. Nanotechnol.

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Adhesion to material surfaces is crucial for almost all organisms regarding subsequent biological responses. Mammalian cell attachment to a surrounding biological matrix is essential for maintaining their survival and function concerning tissue formation. Conversely, the adhesion and presence of microbes interferes with important multicellular processes of tissue development. Therefore, tailoring bioselective, biologically active, and multifunctional materials for biomedical applications is a modern focus of biomaterial research. Engineering biomaterials that stimulate and interact with cell receptors to support binding and subsequent physiological responses of multicellular systems attracted much interest in the last years. Further to this, the increasing threat of multidrug resistance of pathogens against antibiotics to human health urgently requires new material concepts for preventing microbial infestation and biofilm formation. Thus, materials exhibiting microbial repellence or antimicrobial behaviour to reduce inflammation, while selectively enhancing regeneration in host tissues are of utmost interest. In this context, protein-based materials are interesting candidates due to their natural origin, biological activity, and structural properties. Silk materials, in particular those made of spider silk proteins and their recombinant counterparts, are characterized by extraordinary properties including excellent biocompatibility, slow biodegradation, low immunogenicity, and non-toxicity, making them ideally suited for tissue engineering and biomedical applications. Furthermore, recombinant production technologies allow for application-specific modification to develop adjustable, bioactive materials. The present review focusses on biological processes and surface interactions involved in the bioselective adhesion of mammalian cells and repellence of microbes on protein-based material surfaces. In addition, it highlights the importance of materials made of recombinant spider silk proteins, focussing on the progress regarding bioselectivity.

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“…With regard to environmental factors, the advantages of these materials are apparent, as cellulose is derived from regrowing plant material, and microorganisms can produce recombinant spider silk proteins in large-scale bioreactors [ 5 ]. The excellent biocompatibility of cellulose [ 6 , 7 , 8 ], combined with the biocompatibility and shielding properties of recombinant spider silk [ 9 , 10 , 11 ] make these novel optical fibers a promising alternative for medical applications.…”

Section: Introductionmentioning

confidence: 99%

Biocompatible Optical Fibers Made of Regenerated Cellulose and Recombinant Cellulose-Binding Spider Silk

et al. 2023

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The fabrication of green optical waveguides based on cellulose and spider silk might allow the processing of novel biocompatible materials. Regenerated cellulose fibers are used as the core and recombinantly produced spider silk proteins eADF4(C16) as the cladding material. A detected delamination between core and cladding could be circumvented by using a modified spider silk protein with a cellulose-binding domain-enduring permanent adhesion between the cellulose core and the spider silk cladding. The applied spider silk materials were characterized optically, and the theoretical maximum data rate was determined. The results show optical waveguide structures promising for medical applications, for example, in the future.

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Microbial repellence properties of engineered spider silk coatings prevent biofilm formation of opportunistic bacterial strains

Cited by 7 publications

References 51 publications

Compositional Data Analysis of 16S rRNA Gene Sequencing Results from Hospital Airborne Microbiome Samples

Compositional Data Analysis of 16S rRNA Gene Sequencing Results from Hospital Airborne Microbiome Samples

Bioselectivity of silk protein-based materials and their bio-inspired applications

Biocompatible Optical Fibers Made of Regenerated Cellulose and Recombinant Cellulose-Binding Spider Silk

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