Microbial Safety of Foods in the Supply Chain and Food Security

El-Khishin, Mohamed; Gooneratne, Ravi; Hussain, Malik Altaf

doi:10.17140/aftnsoj-3-141

Cited by 14 publications

(10 citation statements)

References 60 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Furthermore, microbial food contamination in the food supply chain cause food losses and foodborne illnesses that result in heavy economic losses (Elkhishin et al, 2017). In Ethiopia, a loss equivalent to 28.45 USD was estimated to arise from every infected slaughtered cattle (Fromsa and Jobre, 2012) while in Egypt, direct economic loss was valued at 28544.3 USD and said to arise from condemnation of meat and liver (Elmonir and Ramadan, 2016).…”

Section: Introductionmentioning

confidence: 99%

Causes of losses and the economic loss estimates at post-harvest handling points along the beef value chain in Uganda

Kyayesimira¹,

Wangalwa²,

Rugunda³

et al. 2019

J. Agric. Ext. Rural Dev

View full text Add to dashboard Cite

Uganda's beef industry has been growing slowly and requires sustained monitoring of actors at postharvest handling points in order to decrease public health risks and losses. This study documented causes of losses and estimated economic values at post-harvest handling points along the beef value chain. It was carried out at slaughter houses, transporters and butcher shops in the districts of Western, Central and Eastern Uganda. A cross sectional study was conducted among meat handlers who were interviewed to find out the losses incurred in the value chain. Microbial load from carcass swabs were collected and evaluated using standard microbiological methods to determine microbial contamination of beef. The causes of losses varied at different handling points. The actors at slaughter houses indicated the major losses were due to low beef demand (15.3%), insecurity (13.4%) and poor weight estimation methods (11.03%). Losses at the butchery included, beef waste (22.4%), drip loss (19.7%) and beef spoilage (18.4%). Microbial analysis showed the highest microbial prevalence at the butchery (70-100%) followed by slaughter (50-80%) and lastly transport (30-50%). Microbial contamination on carcass leads to spoilage and hence market loss because exportation does not admit contaminated foods. Actors reported beef waste and drip loss as the major causes of losses at the butchery. To reduce losses, public health care education for meat handlers and adherence to strict standard operating procedures (SOPs) are a key.

show abstract

Section: Introductionmentioning

confidence: 99%

Causes of losses and the economic loss estimates at post-harvest handling points along the beef value chain in Uganda

Kyayesimira¹,

Wangalwa²,

Rugunda³

et al. 2019

J. Agric. Ext. Rural Dev

View full text Add to dashboard Cite

show abstract

“…Therefore, products are released into the supply chain even before obtaining test results. Such practice is very costly, resulting in more than hundreds of recalls each year and the loss of millions of pounds of food (Buzby et al, 2014;Elkhishin et al, 2017). Therefore, rapid, accurate, and user-friendly viable pathogen detection tools are in high demand to lower recalls, reduce food waste and financial loss, and prevent foodborne outbreaks.…”

Section: Discussionmentioning

confidence: 99%

Mammalian Cell-Based Immunoassay for Detection of Viable Bacterial Pathogens

Bai

Tenguria

et al. 2020

Front. Microbiol.

View full text Add to dashboard Cite

Rapid detection of live pathogens is of paramount importance to ensure food safety. At present, nucleic acid-based polymerase chain reaction and antibody-based lateral flow assays are the primary methods of choice for rapid detection, but these are prone to interference from inhibitors, and resident microbes. Moreover, the positive results may neither assure virulence potential nor viability of the analyte. In contrast, the mammalian cell-based assay detects pathogen interaction with the host cells and is responsive to only live pathogens, but the short shelf-life of the mammalian cells is the major impediment for its widespread application. An innovative approach to prolong the shelf-life of mammalian cells by using formalin was undertaken. Formalin (4% formaldehyde)-fixed human ileocecal adenocarcinoma cell line, HCT-8 on 24-well tissue culture plates was used for the capture of viable pathogens while an antibody was used for specific detection. The specificity of the Mammalian Cell-based ImmunoAssay (MaCIA) was validated with Salmonella enterica serovar Enteritidis and Typhimurium as model pathogens and further confirmed against a panel of 15 S. Enteritidis strains, 8 S. Typhimurium, 11 other Salmonella serovars, and 14 non-Salmonella spp. The total detection time (sample-to-result) of MaCIA with artificially inoculated ground chicken, eggs, milk, and cake mix at 1–10 CFU/25 g was 16–21 h using a traditional enrichment set up but the detection time was shortened to 10–12 h using direct on-cell (MaCIA) enrichment. Formalin-fixed stable cell monolayers in MaCIA provide longer shelf-life (at least 14 weeks) for possible point-of-need deployment and multi-sample testing on a single plate.

show abstract

“…Microbial contamination may take place at prefarming, farming or post-farming stages of the food supply chain. Campylobacter, Salmonella, Listeria monocytogenes, Escherichia coli O157:H7 and non-O157:H7 STEC E. coli are the most common pathogenic bacteria associated with food safety issues in the food supply chain [44]. Efficient process controls and effective food safety management systems are vital elements to reduce microbial contamination and improve food security.…”

Section: Australia and New Zealandmentioning

confidence: 99%