Microbial signature profiles of periodontally healthy and diseased patients

Lourenço, Talita Gomes Baêta; Heller, Débora; Silva-Boghossian, Carina Maciel; Cotton, Sean L.; Paster, Bruce J.; Colombo, Ana Paula Vieira

doi:10.1111/jcpe.12302

Cited by 159 publications

(173 citation statements)

References 40 publications

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“…OT930) were significantly more predominant in diseased individuals compared to PH. Many of these microorganisms are associated with caries, periodontal and endodontic infections [69–72], but they have also been isolated from extra-oral infections or systemic conditions [52–54,73–75]. In contrast, S. australis and G. adiacens have been related to periodontal health [69,70].…”

Section: Discussionmentioning

confidence: 99%

Defining the gut microbiota in individuals with periodontal diseases: an exploratory study

Lourenςo

Spencer

Alm

et al. 2018

Journal of Oral Microbiology

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122

109

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Background: This exploratory study aimed to characterize the gut microbiome of individuals with different periodontal conditions, and correlate it with periodontal inflammation and tissue destruction.Methods: Stool samples were obtained from individuals presenting periodontal health (PH = 7), gingivitis (G = 14) and chronic periodontitis (CP = 23). The intestinal microbiome composition was determined by Illumina MiSeq sequencing.Results: A lower alpha-diversity in the gut microbiome of individuals with CP was observed, although no significant difference among groups was found (p > 0.01). Firmicutes, Proteobacteria, Verrucomicrobia and Euryarchaeota were increased, whereas Bacteroidetes were decreased in abundance in patients with periodontitis compared to PH. Prevotella (genus), Comamonadaceae (family) and Lactobacillales (order) were detected in higher numbers in G, while Bacteroidales (order) was predominant in PH (p < 0.01). Significant correlations (rho = 0.337–0.468, p < 0.01) were found between OTUs representative of periodontal pathogens and attachment loss. Mogibacteriaceae, Ruminococcaceae and Prevotella were able to discriminate individuals with periodontal diseases from PH (overall accuracy = 84%). Oral taxa were detected in high numbers in all stool samples.Conclusions: Individuals with periodontal diseases present a less diverse gut microbiome consistent with other systemic inflammatory diseases. High numbers of oral taxa related to periodontal destruction and inflammation were detected in the gut microbiome of individuals regardless of periodontal status.

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Section: Discussionmentioning

confidence: 99%

Defining the gut microbiota in individuals with periodontal diseases: an exploratory study

Lourenςo

Spencer

Alm

et al. 2018

Journal of Oral Microbiology

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122

109

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“…It is well recognized that there are many qualities of oral biofilm that can lead to the development or suppression of biofilminduced pathogenicity (54)(55)(56). The presence of a few uncultivable species, such as those of the candidate bacterial division TM7, could be highly significant for the manifestation of oral diseases, particularly periodontal disease (57).…”

Section: Discussionmentioning

confidence: 99%

Microbial Diversity in the Early In Vivo -Formed Dental Biofilm

Heller

Helmerhorst

Gower

et al. 2016

Appl Environ Microbiol

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f Although the mature dental biofilm composition is well studied, there is very little information on the earliest phase of in vivo tooth colonization. Progress in dental biofilm collection methodologies and techniques of large-scale microbial identification have made new studies in this field of oral biology feasible. The aim of this study was to characterize the temporal changes and diversity of the cultivable and noncultivable microbes in the early dental biofilm. Samples of early dental biofilm were collected from 11 healthy subjects at 0, 2, 4, and 6 h after removal of plaque and pellicle from tooth surfaces. With the semiquantitative Human Oral Microbiome Identification Microarray (HOMIM) technique, which is based on 16S rRNA sequence hybridizations, plaque samples were analyzed with the currently available 407 HOMIM microbial probes. This led to the identification of at least 92 species, with streptococci being the most abundant bacteria across all time points in all subjects. High-frequency detection was also made with Haemophilus parainfluenzae, Gemella haemolysans, Slackia exigua, and Rothia species. Abundance changes over time were noted for Streptococcus anginosus and Streptococcus intermedius (P ‫؍‬ 0.02), Streptococcus mitis bv. 2 (P ‫؍‬ 0.0002), Streptococcus oralis (P ‫؍‬ 0.0002), Streptococcus cluster I (P ‫؍‬ 0.003), G. haemolysans (P ‫؍‬ 0.0005), and Stenotrophomonas maltophilia (P ‫؍‬ 0.02). Among the currently uncultivable microbiota, eight phylotypes were detected in the early stages of biofilm formation, one belonging to the candidate bacterial division TM7, which has attracted attention due to its potential association with periodontal disease.

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“…In chronic periodontitis, high proportions of the red and orange complexes are observed, while A. actinomycetemcomitans is a major pathogen associated with aggressive forms of periodontitis [83]. However, recent studies have shown that in addition to A. actinomycetemcomitans, aggressive periodontitis may be modulated by a consortia of pathogenic species [85][86][87][88]. Microorganisms associated with other infections in the human body, as well as novel potential pathogenic species, including not-yet-cultivated microorganisms, may also play a role in the initiation, progression, and/or severity of various forms of periodontal diseases [89][90][91][92][93][94][95].…”

Section: Periodontopathogenic Biofilmmentioning

confidence: 98%

Microbiology of Oral Biofilm-Dependent Diseases: Have We Made Significant Progress to Understand and Treat These Diseases?

Colombo

Souto

Silva-Boghossian

et al. 2014

Curr Oral Health Rep

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The oral microbiome plays a crucial role in the establishment and maintenance of oral health. Major disturbances in the interactions between this microbiome, the microenvironment, and the host may lead to the development of oral diseases. A large proportion of infectious diseases that affect humans are caused by organisms living in biofilms. This is also the case for oral infections such as dental caries, periodontal diseases, and endodontic and fungal infections. In this review, new concepts regarding the role of the oral biofilm in the etiopathogenesis of these diseases are presented, and a more ecological view of pathogenic communities as causative agents of oral diseases is discussed. We believe that a better understanding of oral biofilm physiology and ecology will provide insight for the development of new cost-effective diagnostic tools, as well as preventive and therapeutic strategies for the management of these infections.

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Microbial signature profiles of periodontally healthy and diseased patients

Cited by 159 publications

References 40 publications

Defining the gut microbiota in individuals with periodontal diseases: an exploratory study

Defining the gut microbiota in individuals with periodontal diseases: an exploratory study

Microbial Diversity in the Early In Vivo -Formed Dental Biofilm

Microbiology of Oral Biofilm-Dependent Diseases: Have We Made Significant Progress to Understand and Treat These Diseases?

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