Microbial spectrum, antibiotic susceptibility profile, and biofilm formation of diabetic foot infections (2014–18): a retrospective multicenter analysis

Al-Joufi, Fakhria A.; Aljarallah, Khalid M.; Hagras, Soheir A. A.; Hosiny, Ibrahim M. Al; Salem‐Bekhit, Mounir M.; Youssof, Abdullah M. E.; Shakeel, Faiyaz

doi:10.1007/s13205-020-02318-x

Cited by 14 publications

(14 citation statements)

References 27 publications

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“…Indeed, biofilms exhibit enhanced tolerance to antibiotics compared to free-living bacteria, which makes treatment of wound infections challenging [32]. A retrospective study has demonstrated that Gram-negative from DFI were found to be biofilm producers [38]. The results of the present study demonstrated that all P. aeruginosa and Enterobacteriaceae strains produced biofilms.…”

Section: Discussionmentioning

confidence: 51%

Study of Biofilm Formation and Antibiotic Resistance Pattern of Bacteria Isolated from Diabetic Foot Ulcers in Hôpital de Référence Saint Joseph, Kinshasa, Democratic Republic of Congo

Iyamba¹,

Bassom²,

Lukukula³

et al. 2021

AiM

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Foot infections resulting from biofilm producers and multi-drug resistant organisms is one of the most important complications of diabetes mellitus, as it can impede the wound healing process. This study was carried out in order to determine the antibiotic resistance pattern and the biofilm production in diabetic foot ulcers isolates. Clinical samples were collected from patients suffering from diabetic foot ulcers by using sterile swabs. Antibiotic susceptibility test was done using disk diffusion method on Mueller Hinton Agar.Biofilm formation was assessed by Crystal Violet Staining Method. Staphylococcus aureus isolates were resistant to ofloxacin (83.3%), ciprofloxacin (75.0%), trimethoprim-sulphamethoxazole (75.0%), and gentamicin (58.8%) but very sensitive to oxacillin (100.0%) and vancomycin (91.7%). Pseudomonas aeruginosa isolates showed resistance to the commonly used antibiotics such as ofloxacin, cefotaxime, ampicillin (81.8%), ceftazidime and imipenem (72.7%). The majority of bacteria studied were biofilm producers. This study showed that bacteria isolated from diabetic foot ulcers were biofilm producers and presented resistance to commonly used antibiotics. Knowledge on antibiotic sensitivity pattern and biofilm phenotype of the isolates will be helpful in de-How to cite this paper: Liesse Iyamba,

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Section: Discussionmentioning

confidence: 51%

Study of Biofilm Formation and Antibiotic Resistance Pattern of Bacteria Isolated from Diabetic Foot Ulcers in Hôpital de Référence Saint Joseph, Kinshasa, Democratic Republic of Congo

Iyamba¹,

Bassom²,

Lukukula³

et al. 2021

AiM

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“…More recent studies demonstrated higher rates of ESBL, such as the study by Jouhar et al which found an ESBL prevalence of 25% among 314 enterobacteriaceae isolates (Jouhar et al, 2020). Some studies focusing specifically on E. coli found even higher rates of ESBL, ranging from 43.7% to 77.8% (Al-Joufi et al, 2020;Li et al, 2018;Najari et al, 2019).…”

Section: Discussionmentioning

confidence: 98%

“…The proportion of patients infected with MRSA in our study was 9.2%, which is similar to the proportion reported in a small study conducted in our country in the early 2000s (Lysyy et al, 2008). Global prevalence of MRSA infection in DFU patients reported ranges from 7.7%-43.2% in different regions, though rates probably depend on the sample size and patient characteristics, as well as geographical variations in prevalence (Al-Joufi et al, 2020;Son et al, 2017;Zubair et al, 2011).…”

Section: Discussionmentioning

confidence: 99%

Predictors and outcomes of diabetic foot ulcer infection with ESBL-producing bacteria in a large tertiary center

Leibovitch

Cahn

Gellman

et al. 2021

International Journal of Infectious Diseases

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This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

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“…DFIs are usually polymicrobial infections involving aerobic gram-positive cocci, gram-negative bacteria, and anaerobes 58 . A recent multicenter study by Fakhria et al revealed that polymicrobial infection was identified in 48.5% of patients and that the isolated gram-positive pathogen was 46.7%, gram-negative was 38.6%, and anaerobes were 7.9% in 792 diabetic foot patients 60 . The study also showed that the predominant pathogens were S. aureus (22.2%), methicillin-resistant Staphylococcus aureus (7.7%), Enterococcus spp.…”

Section: Biofilms and Dfusmentioning

confidence: 99%

“…(12.8%), Pseudomonas aeruginosa (9.4%), E. coli (7.9%), Klebsiella spp. (7.5%), Proteus mirabilis (8.9%), coagulase-negative staphylococci (CoNS) (6.6%), anaerobic organisms (5.9%), and fungi (2.3%) 60 .…”

Section: Biofilms and Dfusmentioning

confidence: 99%

Neuropeptides, Inflammation, Biofilms, and diabetic Foot Ulcers

Yang

Han

et al. 2021

Exp Clin Endocrinol Diabetes

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A diabetic foot ulcer (DFU) is a serious complication in patients with diabetes mellitus (DM). A DFU is the most common cause of non-traumatic limb amputation, and patients with DFUs have increased mortality rates within 5 years after amputation. DFUs also increase the risk of cardiovascular and cerebrovascular diseases; therefore, with the increasing incidence and prevalence of diabetic foot wounds, DFUs are gradually becoming a major public health problem. The pathophysiology of DFUs is complicated and remains unclear. In recent years, many studies have demonstrated that the pathophysiology of DFUs is especially associated with neuropeptides, inflammation, and biofilms. Neuropeptides, especially substance P (SP) and calcitonin gene-related peptide (CGRP), play an important role in wound healing. SP and CGRP accelerate the healing of cutaneous wounds by promoting neovascularization, inhibiting the release of certain proinflammatory chemokines, regulating macrophage polarization, and so on. However, the expression of SP and CGRP was downregulated in DM and DFUs. DFUs are characterized by a sustained inflammatory phase. Immune cells such as neutrophils and macrophages are involved in the sustained inflammatory phase in DFUs by extracellular traps (NETs) and dysregulated macrophage polarization, which delays wound healing. Furthermore, DFUs are at increased risk of biofilm formation. Biofilms disturb wound healing by inducing a chronic inflammatory response, inhibiting macrophage phagocytosis and keratinocyte proliferation migration, and transferring antimicrobial resistance genes. To understand the relationships among neuropeptides, inflammation, biofilms, and DFUs, this review highlights the recent scientific advances that provide possible pathophysiological insights into the delayed healing of DFUs.

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Microbial spectrum, antibiotic susceptibility profile, and biofilm formation of diabetic foot infections (2014–18): a retrospective multicenter analysis

Cited by 14 publications

References 27 publications

Study of Biofilm Formation and Antibiotic Resistance Pattern of Bacteria Isolated from Diabetic Foot Ulcers in Hôpital de Référence Saint Joseph, Kinshasa, Democratic Republic of Congo

Study of Biofilm Formation and Antibiotic Resistance Pattern of Bacteria Isolated from Diabetic Foot Ulcers in Hôpital de Référence Saint Joseph, Kinshasa, Democratic Republic of Congo

Predictors and outcomes of diabetic foot ulcer infection with ESBL-producing bacteria in a large tertiary center

Neuropeptides, Inflammation, Biofilms, and diabetic Foot Ulcers

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Microbial spectrum, antibiotic susceptibility profile, and biofilm formation of diabetic foot infections (2014–18): a retrospective multicenter analysis

Cited by 14 publications

References 27 publications

Study of Biofilm Formation and Antibiotic Resistance Pattern of Bacteria Isolated from Diabetic Foot Ulcers in H&#244;pital de R&#233;f&#233;rence Saint Joseph, Kinshasa, Democratic Republic of Congo

Study of Biofilm Formation and Antibiotic Resistance Pattern of Bacteria Isolated from Diabetic Foot Ulcers in H&#244;pital de R&#233;f&#233;rence Saint Joseph, Kinshasa, Democratic Republic of Congo

Predictors and outcomes of diabetic foot ulcer infection with ESBL-producing bacteria in a large tertiary center

Neuropeptides, Inflammation, Biofilms, and diabetic Foot Ulcers

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Study of Biofilm Formation and Antibiotic Resistance Pattern of Bacteria Isolated from Diabetic Foot Ulcers in Hôpital de Référence Saint Joseph, Kinshasa, Democratic Republic of Congo

Study of Biofilm Formation and Antibiotic Resistance Pattern of Bacteria Isolated from Diabetic Foot Ulcers in Hôpital de Référence Saint Joseph, Kinshasa, Democratic Republic of Congo