Microbial stress response to heavy metals in the environment

Prabhakaran, Pranesha; Ashraf, Muhammad Aqeel; Aqma, Wan Syaidatul

doi:10.1039/c6ra10966g

Cited by 166 publications

(85 citation statements)

References 75 publications

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“…Further transcriptional tests were carried out using additional metal compounds (CuSO 4 , MnCl 2 , NiSO 4 , and FeSO 4 ), as these metals persist in the aquatic system and can trigger various biological responses in the exposed organisms . Similar to CuCl 2 , CuSO 4 treatment also increased the CeCSD1 and CeCSD2 gene expression, resulting in 3.2‐ and 2.6‐fold increases at 0.2 mg/L CuSO 4 , respectively; following which, the expressions decreased (Figure A).…”

Section: Resultsmentioning

confidence: 99%

Molecular characterization and expression analysis of copper‐zinc superoxide dismutases from the freshwater alga Closterium ehrenbergii under metal stress

Wang

2019

Environmental Toxicology

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Superoxide dismutase (SOD) acts as the first line of defense against reactive oxygen species (ROS) within cells. In the present study, we determined two novel CuZnSOD genes (designated as CeCSD1 and CeCSD2) from the toxicity-testing freshwater algae Closterium ehrenbergii and examined their structural features, phylogenetic relationships, and gene expression under exposure to different metals. Putative CeCSD1 (204 aa, 20.6 kDa) and CeCSD2 (155 aa, 15.3 kDa) proteins had conserved CuZnSOD family motifs and metal (Cu, Zn) binding sites, but different N-terminus structures, that is, CeCSD1 has a signal peptide to chloroplasts. Phylogenetic analysis of each protein revealed that C. ehrenbergii was well clustered with other green algae and plants. Real-time PCR results showed that the gene expression obviously increased with heavy metal exposure. In addition, excess copper considerably increased the SOD activity and ROS generation but decreased the photosynthetic efficiency in treated cells. These results suggest that CeCSDs are involved in the antioxidant defense system and can be regarded as potential biomarkers for monitoring metal contaminants in aquatic environments. K E Y W O R D SCeCSD, Closterium ehrenbergii, gene expression, heavy metals, ROS

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Section: Resultsmentioning

confidence: 99%

Molecular characterization and expression analysis of copper‐zinc superoxide dismutases from the freshwater alga Closterium ehrenbergii under metal stress

Wang

2019

Environmental Toxicology

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“…According to Gupta and Diwan (2017), the synthesis of EPS is one of the reactions of microorganism cells to the presence of heavy metals [36]. This was also confirmed by numerous studies by other researchers [37,38]. Wang et al (2010) explains the phenomenon of sorption by the interaction between positively charged metal ions and negatively charged EPS [39].…”

Section: Discussionmentioning

confidence: 80%

Heavy Metals as a Factor Increasing the Functional Genetic Potential of Bacterial Community for Polycyclic Aromatic Hydrocarbon Biodegradation

Staninska-Pięta

Czarny²,

Piotrowska-Cyplik

et al. 2020

Molecules

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The bioremediation of areas contaminated with hydrocarbon compounds and heavy metals is challenging due to the synergistic toxic effects of these contaminants. On the other hand, the phenomenon of the induction of microbial secretion of exopolysaccharides (EPS) under the influence of heavy metals may contribute to affect the interaction between hydrophobic hydrocarbons and microbial cells, thus increasing the bioavailability of hydrophobic organic pollutants. The purpose of this study was to analyze the impact of heavy metals on the changes in the metapopulation structure of an environmental consortium, with particular emphasis on the number of copies of orthologous genes involved in exopolysaccharide synthesis pathways and the biodegradation of hydrocarbons. The results of the experiment confirmed that the presence of heavy metals at concentrations of 50 mg·L−1 and 150 mg·L−1 resulted in a decrease in the metabolic activity of the microbial consortium and its biodiversity. Despite this, an increase in the biological degradation rate of polycyclic aromatic hydrocarbons was noted of 17.9% and 16.9%, respectively. An assessment of the estimated number of genes crucial for EPS synthesis and biodegradation of polycyclic aromatic hydrocarbons confirmed the relationship between the activation of EPS synthesis pathways and polyaromatic hydrocarbon biodegradation pathways. It was established that microorganisms that belong to the Burkholderiales order are characterized by a high representation of the analyzed orthologs and high application potential in areas contaminated with heavy metals and hydrocarbons.

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“…and Penicillium sp. make it exists in the surrounding longer than the heavier and bigger spores (Vonberg & Gastmeier, 2006), and this type of spores can cause the asthma to human (Pastuszka et al, 2000;Prabhakaran et al 2016). Health problem that cause by fungus is usually due to is mycotoxin.…”

Section: Discussionmentioning

confidence: 99%

Assessment of Indoor Microbiological Air Contamination in Research Facility at University of Malaysia

Mazlan

Hamzah

Aqma

et al. 2020

Preprint

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Indoor air quality is a concept that applies to the nature of the environment in and around buildings and facilities in which it contributes to the safety and security of those in the house. The aim of this study was to identify bacterial and fungal species present in the room, determine indoor air quality and investigate consumer views on indoor air quality in the Biology Building, Faculty of Sciences and Technology, National University of Malaysia. This study was conducted at the Biology Building at 8 selected sampling sites. Microbes were sampled using nutrient agar (bacteria) and potato dextrose agar (fungus). All samples of microbes were classified using two approaches; i) morphological examination and ii) biochemical reaction experiments. CO2, temperature and relative humidity are registered using the Direct Sense Monitoring Kit. A survey on customer satisfaction with indoor air quality in the building was carried out and evaluated in order to collect empirical details. This study found that the presence of Bacillus cereus, Bacillus laterosporus, Bacillus sphaericus, Micrococcus luteus, Staphylococcus aureus, Staphylococcus epidermis, Enterobacter cloacae, Pseudomonas fluarescens, Pseudomonas stuzeri and Aeromonas hydrophila bacterial. The fungi species are Aspergillus niger, Aspergillus nidulans, Penicillium digitatum and Fusarium dimerum. The result also shows that the carbon dioxide, temperature and relative humidity concentrations for most sampling stations comply with the DOSH standards. Moreover, almost all participants reported that their level of health and comfort while in the building is good, while the ventilation system of the building is at a comfortable level. Whereas, the degree of knowledge for most respondents on the value of indoor air quality is high. Two of the recommendations included in this study to enhance indoor air quality are to insure that the air conditioning device is correctly controlled and to raise understanding of the value of indoor air quality among staff and students in the Biology Building.

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Microbial stress response to heavy metals in the environment

Abstract: Heavy metal contamination is a global environmental issue as it poses a significant threat to public health, and exposure to metals above a certain threshold level can cause deleterious effects in all living organisms including microbes.

Cited by 166 publications

References 75 publications

Molecular characterization and expression analysis of copper‐zinc superoxide dismutases from the freshwater alga Closterium ehrenbergii under metal stress

Molecular characterization and expression analysis of copper‐zinc superoxide dismutases from the freshwater alga Closterium ehrenbergii under metal stress

Heavy Metals as a Factor Increasing the Functional Genetic Potential of Bacterial Community for Polycyclic Aromatic Hydrocarbon Biodegradation

Assessment of Indoor Microbiological Air Contamination in Research Facility at University of Malaysia

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