Microbial tannase: Current perspectives and biotechnological advances

Govindarajan, Rasiravathanahalli Kaveriyappan; Revathi, S.; Rameshkumar, Neelamegam; Krishnan, Muthukalingan; Kayalvizhi, Nagarajan

doi:10.1016/j.bcab.2016.03.011

Cited by 59 publications

(40 citation statements)

References 74 publications

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“…Tannase has received a great deal of attention from the discovery, and it is widely used in food, animal feed, pharmaceutical, beverage, brewing, tannery and chemical industry (Govindarajan et al, 2016). As well as gallic acid, the major hydrolytic product of tannic acid is used in food, cosmetics and adhesive in the synthesis of a potent antioxidant and propylgallate (Aithal & Belur, 2013) .…”

Section: Introductionmentioning

confidence: 99%

Implementation of Different Fermentation Techniques for Induction of Tannase and Gallic Acid Using Agro-residues Substrates

Ahmed

Abou-Taleb

2019

Egypt. J. Microbiol.

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T ANNASE is an inducible enzyme which hydrolysate tannin to gallic acid and used to in many industries such as food and pharmaceutical. This enzyme has scooped more attention in recent times. A maximum production of tannase (ranged from 0.30 to 0.93 enzyme index and from1.05 to 1.87U/mg specific tannase activity (STA)) and gallic acid (from 0.07 to 0.76mg/ml gallic acid concentration (GAC)) was achieved by 8 fungal strains out of 24 fungal and yeast strains belonged to genera; Aspergillus, Rhizopus, Trichoderma, Fusarium, Penicillium, Candida and Saccharomyces. The selected fungi were grown on tannin-rich substrates (eucalyptus leaves, pomegranate peel, banana peel, guava leaves and wheat bran) and by-products (corn steep liquor (CSL) and soybean extract) as sole carbon and nitrogen sources for tannase and GA production at 28°C for 6 days under liquid-surface (LSF), submerged (SmF) and solid-state (SSF) fermentation. Results indicated that A. niger A8 and T. viride with 10% (v/v) of inoculum size gave a high STA (from 8.08 to 10.95U/mg) and GAC (from 2.62 to 4.00mg/ml) on pomegranate and banana peels supplemented with CSL after 4 days incubation at 28°C under SSF compared to SmF and LSF. STA (ranged from 10.68 to 12.93U/mg) and GAC (ranged from 3.56 to 4.16mg/ml) were increased when inoculated the medium with both A. niger A8+T. viride (with 5:5% v/v of inoculum size) more than when inoculated with each of them separately.

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Section: Introductionmentioning

confidence: 99%

Implementation of Different Fermentation Techniques for Induction of Tannase and Gallic Acid Using Agro-residues Substrates

Ahmed

Abou-Taleb

2019

Egypt. J. Microbiol.

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“…Tannase (or tannin acyl hydrolase, EC 3.1.1.20) has mostly been characterised by its hydrolysing activity on the ester bond (galloyl ester of an alcohol moiety) and the depside bond (galloyl ester of gallic acid) of substrates such as tannic acid, EGCG, ECG and chlorogenic acid (Schons et al, 2012;Govindarajan et al, 2016). It has many potential applications, and the most important one is catalysing the biotransformation of tea catechins, resulting in increased antioxidant activities (Baik et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Polyphenolic composition and in vitro antioxidant activities of native‐ and tannase‐treated green tea extracts

Ong

Annuar

2017

Int J of Food Sci Tech

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Summary The antioxidant activities of native‐ and tannase‐treated green tea extracts along with their major polyphenol components were investigated. The polyphenolic content and composition of the tea before and after tannase treatment were determined by liquid chromatography coupled with mass spectrometry (LC‐MS). Approximately 99% of the (−)‐epigallocatechin gallate (EGCG) and (−)‐epicatechin gallate (ECG) in green tea extract were converted by tannase to (−)‐epigallocatechin (EGC) and (−)‐epicatechin (EC), respectively, after 30 min. Biotransformed green tea exhibited a significantly higher DPPH˙ radical scavenging activities than native green tea (EC50 value of 0.024 ± 0.001 and 0.044 ± 0.001 mg mL−1, respectively). Kinetic parameters such as scavenging rate and stoichiometry were calculated. The rate of DPPH˙ radical scavenging activities for tannase‐treated green tea extract was shown to be higher than native green tea extract.

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“…However, a major problem in usage of fungal strain at industrial level is its relatively slow growth rate and genetic complexity. However, bacterial genera such as Bacillus, Streptococcus gallolyticus, Klebsiella, Lactobacillus and Pantonea have also the ability to degrade and hydrolyze natural tannins and tannic acid very efficiently [3,4]. On the other hand, the growth rate of bacteria is very high and they can be easily manipulated at the genetic level.…”

Section: Introductionmentioning

confidence: 99%

Digestion of Tannin by Bacteria Enterobacter cloacae from the Gut of Indian Mole Cricket (Gryllotalpa krishnani)

RK¹,

Revathi²,

Rameshkumar³

et al. 2017

J Bioprocess Biotech

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Insects are the most successful animal on earth; the gut microbes present might play an important role in food digestion as well as the interaction with hosts. In this study insect Gryllotalpa krishnani has been used to isolate the symbiotic associated organisms which play role in host metabolism, promote efficient digestion and to protect the host from the harmful microbes. In the present study, microorganisms were isolated from the gut of G. krishnani and characterized for tannase enzyme activity. It was observed that 5 isolates (TAH 6, TAH 13, TAH 36, TAH 38 and TAH 41) out of total 120 tested were able to show significant growth on tannic acid plate. Among these five potential isolates, strain TAH 41 exhibited maximum tannase activity in tannase plate assay and was selected for the further study. The bacterial strain TAH41 was analyzed for biochemical analysis, 16S rDNA sequencing and the result confirmed the strain as Enterobacter cloacae. HPLC analysis showed the formation two peaks representing gallic acid and glucose as a by-product FT-IR analysis also confirmed the same. The polyacrylamide gel electrophoresis (SDS-PAGE) and zymogram analysis showed the molecular mass of tannase enzyme in cell free extract was ~45 kDa, the analysis suggested this tannase enzyme to be one of the smallest of the bacterial source, which could be attributed to the formation of di or tri-galloyl glucose. The present study was the first report E. cloacae with tannase activity from G. krishnani gut. E. cloacae which may endow the insect with some ecological advantages by enabling them to overcome the anti-nutritional effects of plant tannins.Citation: Rasiravuthanahalli KG, Revathi S, Rameshkumar N, Krishnan M, Kayalvizhi N (2017) Digestion of Tannin by Bacteria Enterobacter cloacae from the Gut of Indian Mole Cricket (Gryllotalpa krishnani).

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Microbial tannase: Current perspectives and biotechnological advances

Cited by 59 publications

References 74 publications

Implementation of Different Fermentation Techniques for Induction of Tannase and Gallic Acid Using Agro-residues Substrates

Implementation of Different Fermentation Techniques for Induction of Tannase and Gallic Acid Using Agro-residues Substrates

Polyphenolic composition and in vitro antioxidant activities of native‐ and tannase‐treated green tea extracts

Digestion of Tannin by Bacteria Enterobacter cloacae from the Gut of Indian Mole Cricket (Gryllotalpa krishnani)

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