Microbial transformation of furfural to furfuryl alcohol by saccharomyces cerevisiae

Villa, GA; Bartroli, R.; López, Rosa; Guerra, Marisa; Enrique, María; Peñas, M. Solchaga-Las; Rodriquez, Enrique; Redondo, D. Fernández; Jglesias, I.; Ríos, Manuel Díaz de los

doi:10.1002/abio.370120613

Cited by 84 publications

(36 citation statements)

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“…The in vitro activity for furfural reduction by a The conversion rates were calculated based on consumption of furfural or HMF during the feeding of hydrolysate. The in vitro furfural-and HMF-reducing activities were measured as described previously (23).…”

Section: Resultsmentioning

confidence: 99%

“…Strains of S. cerevisiae exhibit significant differences in fermentative capacity and tolerance to lignocellulose-derived inhibitors (11). The tolerance to aldehyde compounds is most likely due to the ability of microorganisms to convert these compounds to the corresponding less inhibitory alcohols (20,23). Dilute-acid hydrolysates are often strongly inhibiting and cannot be fermented in batch mode, but they may be fermented by S. cerevisiae without prior detoxification in fed-batch mode (13,14,21).…”

Section: -Hydroxymethyl Furfural (400 Mu/mg Protein During Fed-batch mentioning

confidence: 99%

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Cofactor Dependence in Furan Reduction by Saccharomyces cerevisiae in Fermentation of Acid-Hydrolyzed Lignocellulose

Nilsson

Gorwa-Grauslund

Hahn‐Hägerdal

et al. 2005

Appl Environ Microbiol

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A decreased fermentation rate due to inhibition is a significant problem for economic conversion of acid-pretreated lignocellulose hydrolysates to ethanol, since the inhibition gives rise to a requirement for separate detoxification steps. Together with acetic acid, the sugar degradation products furfural and 5-hydroxymethyl furfural are the inhibiting compounds found at the highest concentrations in hydrolysates. These aldehydes have been shown to affect both the specific growth rate and the rate of fermentation by yeast. Two strains of Saccharomyces cerevisiae with different abilities to ferment inhibiting hydrolysates were evaluated in fermentations of a dilute acid hydrolysate from spruce, and the reducing activities for furfural and 5-hydroxymethyl furfural were determined. Crude cell extracts of a hydrolysate-tolerant strain (TMB3000) converted both furfural and 5-hydroxymethyl furfural to the corresponding alcohol at a rate that was severalfold higher than the rate observed for cell extracts of a less tolerant strain (CBS 8066), thereby confirming that there is a correlation between the fermentation rate in a lignocellulosic hydrolysate and the bioconversion capacity of a strain. The in vitro NADH-dependent furfural reduction capacity of TMB3000 was three times higher than that of CBS 8066 (1,200 mU/mg protein and 370 mU/mg protein, respectively) in fed-batch experiments. Furthermore, the inhibitor-tolerant strain TMB3000 displayed a previously unknown NADH-dependent reducing activity for 5-hydroxymethyl furfural (400 mU/mg protein during fed-batch fermentation of hydrolysates). No corresponding activity was found in strain CBS 8066 (<2 mU/mg). The ability to reduce 5-hydroxymethyl furfural is an important characteristic for the development of yeast strains with increased tolerance to lignocellulosic hydrolysates.

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Section: Resultsmentioning

confidence: 99%

Section: -Hydroxymethyl Furfural (400 Mu/mg Protein During Fed-batch mentioning

confidence: 99%

Cofactor Dependence in Furan Reduction by Saccharomyces cerevisiae in Fermentation of Acid-Hydrolyzed Lignocellulose

Nilsson

Gorwa-Grauslund

Hahn‐Hägerdal

et al. 2005

Appl Environ Microbiol

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“…HMF and furfural could be consumed during yeast fermentation, while the rate of furfural consumption was a little faster. Furfural could be reduced by yeast cells to furfuryl alcohol during fermentation and cause a lag-phase in the ethanol formation but it does not affect the final ethanol yield [28]. The minimum concentration of furfural and HMF that could be tested was 0.2 g/L in the HPLC system used in this study.…”

Section: Fermentationmentioning

confidence: 99%

Ethanol production from corn stover pretreated by electrolyzed water and a two-step pretreatment method

Wang

Feng

2012

Chin. Sci. Bull.

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Pretreatment is one of the most important unit operations for ethanol production from biomass feedstocks. In this study, corn stover was used as a feedstock to examine the effectiveness of two pretreatments: electrolyzed water pretreatment and a two-step pretreatment. Electrolyzed water was employed as a catalyst to conduct one-step pretreatment of corn stover at three temperatures (165, 180 and 195°C) and three treatment times (10, 20 and 30 min). During the two-step pretreatment process, an organic alkaline solution of 1% (w/w) NaOH in 70% (w/w) ethanol was used for lignin removal in the first step, followed by a second step using hot water. No furfural or 5-hydroxymethyl furfural was detected in the hydrolysates from both pretreatment methods when the detection limit of the HPLC was 0.2 g/L. The highest glucan conversion yields were 83% obtained at 195°C for 30 min with acidic electrolyzed water and 83% by the two-step process, where the second step of the pretreatment was at 135°C for 30 min. The hydrolyzates from the two pretreatment methods showed good performance in Saccharomyces cerevisiae fermentation tests. The two new methods may provide promising alternatives for the pretreatment of biomass for ethanol production. ethanol, biomass, pretreatment, inhibitor Citation:Wang X J, Feng H, Li Z Y. Ethanol production from corn stover pretreated by electrolyzed water and a two-step pretreatment method.

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“…Previous studies have shown that in most cases, furfural can be converted by yeast to furfural alcohol (12,30). Sometimes furoic acid (64), furoin and furil (47), and acyloin products (28,61) can also be detected in the medium under different sets of cultivation conditions. The genetic mechanisms involved in furfural tolerance have been investigated by screening an S. cerevisiae disruption library to find potential relative genes (18).…”

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confidence: 99%

Comparative Proteomic Analysis of Tolerance and Adaptation of EthanologenicSaccharomyces cerevisiaeto Furfural, a Lignocellulosic Inhibitory Compound

Lin

Qiao

Yang

2009

Appl Environ Microbiol

100

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The molecular mechanism involved in tolerance and adaptation of ethanologenic Saccharomyces cerevisiae to inhibitors (such as furfural, acetic acid, and phenol) represented in lignocellulosic hydrolysate is still unclear. Here, 18 O-labeling-aided shotgun comparative proteome analysis was applied to study the global protein expression profiles of S. cerevisiae under conditions of treatment of furfural compared with furfural-free fermentation profiles. Proteins involved in glucose fermentation and/or the tricarboxylic acid cycle were upregulated in cells treated with furfural compared with the control cells, while proteins involved in glycerol biosynthesis were downregulated. Differential levels of expression of alcohol dehydrogenases were observed. On the other hand, the levels of NADH, NAD ؉ , and NADH/NAD ؉ were reduced whereas the levels of ATP and ADP were increased. These observations indicate that central carbon metabolism, levels of alcohol dehydrogenases, and the redox balance may be related to tolerance of ethanologenic yeast for and adaptation to furfural. Furthermore, proteins involved in stress response, including the unfolded protein response, oxidative stress, osmotic and salt stress, DNA damage and nutrient starvation, were differentially expressed, a finding that was validated by quantitative real-time reverse transcription-PCR to further confirm that the general stress responses are essential for cellular defense against furfural. These insights into the response of yeast to the presence of furfural will benefit the design and development of inhibitor-tolerant ethanologenic yeast by metabolic engineering or synthetic biology.

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Microbial transformation of furfural to furfuryl alcohol by saccharomyces cerevisiae

Cited by 84 publications

References 4 publications

Cofactor Dependence in Furan Reduction by Saccharomyces cerevisiae in Fermentation of Acid-Hydrolyzed Lignocellulose

Cofactor Dependence in Furan Reduction by Saccharomyces cerevisiae in Fermentation of Acid-Hydrolyzed Lignocellulose

Ethanol production from corn stover pretreated by electrolyzed water and a two-step pretreatment method

Comparative Proteomic Analysis of Tolerance and Adaptation of EthanologenicSaccharomyces cerevisiaeto Furfural, a Lignocellulosic Inhibitory Compound

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