Microbial Transformations of Plant Secondary Metabolites

Mutafova, Blaga; Fernandes, Pedro; Mutafov, S.; Berkov, Strahil; Pavlov, Atanas

doi:10.1007/978-3-319-32004-5_15-1

Cited by 3 publications

(4 citation statements)

References 199 publications

(233 reference statements)

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“…Fungi can biotransform terpenoids in particular product(s) and at specific rate(s) through multiple reactions strongly influenced by the cultivation conditions (Frija et al, 2011; Ghasemi et al, 2014; Mutafova et al, 2016). However, production yields of each distinct product from a stereo-selective hydroxylation of the terpenoid are usually very low (2–20%).…”

Section: Discussionmentioning

confidence: 99%

“…In most studies reported so far, the terpenoid containing medium is inoculated with mycelia (previously grown in a standard growth medium) and agitated batch or fed-batch cultivation conditions are used (Frija et al, 2011; Schrader and Bohlmann, 2015; Mutafova et al, 2016). Terpenoids are known to play major roles in plant defense mechanisms (Gershenzon and Dudareva, 2007; Mutafova et al, 2016), probably explaining why the LA plant extract inhibited the germination of P. janczewskii conidia in the agitated cultures.…”

Section: Discussionmentioning

confidence: 99%

“…Terpenoids are known to play major roles in plant defense mechanisms (Gershenzon and Dudareva, 2007; Mutafova et al, 2016), probably explaining why the LA plant extract inhibited the germination of P. janczewskii conidia in the agitated cultures. In our previous study, even at static conditions, germination of conidia was only observed in three out of the eight fungal strains tested (Frija et al, 2011).…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, biotransformation constitutes a reference method for producing new terpenoid derivatives with improved properties (Silva et al, 2013; Schrader and Bohlmann, 2015). The capacity of fungi, as well as bacteria, to transform terpenoids yielding stereo- and regio-selective products and/or compounds functionalized at particular positions has been well documented (e.g., Frija et al, 2011; Mutafova et al, 2016). In a previous study, we reported that three Ascomycota fungal strains (out of the eight tested) were able to transform LA (Frija et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

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Proteomic Insights on the Metabolism of Penicillium janczewskii during the Biotransformation of the Plant Terpenoid Labdanolic Acid

Martins

Varela

Frija

et al. 2017

Front. Bioeng. Biotechnol.

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Plant terpenoids compose a natural source of chemodiversity of exceptional value. Many of these compounds own biological/pharmacological activity, others are regarded as unique chemical skeletons for the synthesis of derivatives with improved properties. Functional chemical modification of terpenoids through biotransformation frequently relies on the use of Ascomycota strains, but information on major cellular responses is still largely lacking. Penicillium janczewskii mediates a stereo-selective hydroxylation of labdanolic acid (LA)—terpenoid found abundantly in Cistus ladanifer—producing 3β-hydroxy-labdanolic acid with yields >90%. Herein, combined analyses of mycelial and extracellular differential proteomes demonstrated that the plant terpenoid increased stress responses, especially against oxidative stress (e.g., accumulation of superoxide dismutase) and apparently altered mitochondria functioning. One putative cytochrome P450 monooxygenase differentially accumulated in the secretome and the terpenoid bioconversion was inhibited in vivo in the presence of a P450 inhibitor. The stereo-selective hydroxylation of the plant terpenoid is likely mediated by P450 enzymes, yet its unequivocal identity remains unclear. To the best of our knowledge, this is the first time that proteomics was used to investigate how a plant terpenoid impacts the metabolism of a filamentous fungus during its efficiently biotransformation. Our findings may encourage the development of new strategies for the valorization of plant natural resources through biotechnology.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Proteomic Insights on the Metabolism of Penicillium janczewskii during the Biotransformation of the Plant Terpenoid Labdanolic Acid

Martins

Varela

Frija

et al. 2017

Front. Bioeng. Biotechnol.

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Food additives and bioactive substances from in vitro systems of edible plants from the Balkan peninsula

Vrancheva

Ivanov

Aneva

et al. 2018

Engineering in Life Sciences

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During the last few years there is an increasing demand to the natural biologically active compounds. According to the World Health Organization (WHO) about 11% of the conventional medicines are of plant origin. Nowadays, plant biotechnologies are modern and reliable tool for producing valuable bioactive compounds. Recently, the potential of plant cells as foods also was confirmed. The advantages of plant in vitro systems over the intact plants are well known: growing under controlled and optimized laboratory conditions; independence of climatic and soil differences; preservation of rare and endangered plant species; cultivation in diverse bioreactor systems for increasing production yields of target metabolites. There have been developed many in vitro systems for production of various plant bioactive compounds with potential application in food industries. But potential for industrial implementation of this technology depends on solving problems with the scale‐up of bioreactor cultivation, development of additional approaches for improving/modification of bioactivities of the target plant secondary metabolites, and to find way to exclude or replace in the culture media the carcinogenic plant growth regulator 2,4‐dichlorophenoxyacetic acid (2,4‐D) with its safety analogs, such as α‐naphtaleneacetic acid (NAA) and/or indole‐3‐butyric acid (IBA). The aim of the current mini review is to summarize information about different in vitro systems of edible plants from the Balkan Peninsula with potential for producing food additives and biologically active substances and to describe prospects for successful industrial implementation of this technology.

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Highly Efficient Biodegradation of Ecotoxic Dehydroabietic Acid by Resting Cells of Rhodococcus rhodochrous IEGM 107

2022

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Microbial Transformations of Plant Secondary Metabolites

Cited by 3 publications

References 199 publications

Proteomic Insights on the Metabolism of Penicillium janczewskii during the Biotransformation of the Plant Terpenoid Labdanolic Acid

Proteomic Insights on the Metabolism of Penicillium janczewskii during the Biotransformation of the Plant Terpenoid Labdanolic Acid

Food additives and bioactive substances from in vitro systems of edible plants from the Balkan peninsula

Highly Efficient Biodegradation of Ecotoxic Dehydroabietic Acid by Resting Cells of Rhodococcus rhodochrous IEGM 107

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