Microbiological analysis of seawater and sediments in urban shorelines: Occurrence of heavy metals resistance bacteria on Chennai beaches, Bay of Bengal

Santhiya, G.; Lakshumanan, C.; Selvin, Joseph; Asha, D.

doi:10.1016/j.microc.2011.05.004

Cited by 25 publications

(10 citation statements)

References 21 publications

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“…The patterns of metal resistance were varying depending on their five different (5, 10, 50, 100 and 250 mM) concentrations. Average concentrations of heavy metals in seawater and sediments were about 50 mM [23]. In the present study, the tube dilution (or) minimal inhibitory concentration (MIC) method confirmed that only few of the 120 isolates could survive at higher (250 mM) concentrations of copper among the remaining isolates.…”

Section: Resultssupporting

confidence: 72%

“…growth against high copper concentrations (250 mM). Heavy metals generally inhibit microbial growth by blocking essential functional groups, displacing essential metal ions, or modifying the active conformation of biological molecules [23]. The Pseudomonas isolates from fishing harbor areas were much more resistant than in other areas which received different waste materials from shipping activities, domestic waste from slums, paintings, cargo/ ballast water discharges.…”

Section: Discussionmentioning

confidence: 99%

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Biomonitoring along the Tropical Southern Indian Coast with Multiple Biomarkers

et al. 2016

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We assessed the spatial and temporal variations of pollution indicators and geochemical and trace metal parameters (23 in total) from water and sediment (144 samples) of three different eco-niches (beach, fishing harbor, and estuary) in larger coastal cities of southern India (Cuddalore and Pondicherry) for one year. A total of 120 marine Pseudomonas isolates were challenged against different concentrations of copper solutions and 10 different antibiotics in heavy metal and antibiotic resistance approaches, respectively. The study shows that 4.16% of the isolates could survive in 250 mM of copper; 70% were resistant to minimum concentrations. Strains were resistant (98.4%) to at least one antibiotic in Cuddalore compared to the Pondicherry (78.4%) region. Pollution index (PI) (0–14.55) and antibiotic resistance index (ARI) (0.05–0.10) ratio indicated that high bacterial and antibiotic loads were released into the coastal environment. The degree of trace metal contamination in sediments were calculated by enrichment factor (EF), contamination factor (CF), pollution load index (PLI), and geo-accumulation index (Igeo). Statistical parameters like two-way analysis of variance (ANOVA), correlation, factor analysis and scatter matrix tools were employed between the 23 parameters in order to find sources, pathways, disparities and interactions of environmental pollutants. It indicates that geochemical and biological parameters were not strongly associated with each other (except a few) and were affected by different sources. Factor analysis elucidated, ‘microbe–metal’ interaction (Factor 1–48.86%), ‘anthropogenic’ factor (Factor 2–13.23%) and ‘Pseudomonas–Cadmium’ factor (Factor 3–11.74%), respectively.

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Section: Resultssupporting

confidence: 72%

Section: Discussionmentioning

confidence: 99%

Biomonitoring along the Tropical Southern Indian Coast with Multiple Biomarkers

et al. 2016

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“…aureus, E. coli, V. cholerae, Y. enterocolitica and C. jejuni (US EPA 1988;WHO 1993;Santhiya et al 2011). Discharge of polluted effluent frequently contaminated sea life, particularly fish, cockles and prawns; therefore, people who eat these contaminated seafood could become seriously ill (WHO 2001).…”

Section: Disposal and Reuse Of Treated Sewage And Biosolidsmentioning

confidence: 99%

“…Discharge of polluted effluent frequently contaminated sea life, particularly fish, cockles and prawns; therefore, people who eat these contaminated seafood could become seriously ill (WHO 2001). Santhiya et al (2011) revealed that the seawater and sediments polluted with discharged treated sewage in Morocco were heavily contaminated with Vibrio sp., Pseudomonas sp., Salmonella spp., Shigella sp. and coliforms group.…”

Section: Disposal and Reuse Of Treated Sewage And Biosolidsmentioning

confidence: 99%

Removal of pathogenic bacteria from sewage-treated effluent and biosolids for agricultural purposes

et al. 2018

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The reuse of treated sewage for irrigation is considered as an important alternative water source in the new water management strategy of the countries that face a severe deficiency of water resources such as the Middle East countries. The organic material and fertilizing elements contained in biosolids are essential for maintaining soil fertility. However, both treated sewage and biosolids contain a large diversity of pathogens that would be transmitted to the environment and infect human directly or indirectly. Therefore, those pathogens should be reduced from the treated sewage and biosolids before the reuse in the agriculture. This paper reviews the considerations for reuse of treated sewage and biosolids in agriculture and further treatments used for reduction of pathogenic bacteria. The treatment methods used for the reduction of pathogens in these wastes have reviewed. It appeared that the main concern associated with the reduction of pathogenic bacteria lies in their ability to regrow in the treated sewage and biosolids. Therefore, the effective treatment method is that it has the potential to destruct pathogens cells and remove the nutrients to prevent the regrowth or recontamination from the surrounded environment. The removal of nutrients might be applicable in the sewage but not in the biosolids due to high nutrient contents. However, the reduction of health risk in the biosolids might be carried out by regulating the biosolid utilization and selecting the plant species grown in the fertilized soil with biosolids.

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“…Marine environments are a reservoir of relevant information on dangerous contaminants such as hydrocarbons, as well as microbial communities with probable degradation skills. Moreover, the information generated can be used as a biological indicator for the characterization of aqueous pollution (Santhiya et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Research Article Inexpensive metagenomic DNA extraction protocol with high quality from marine sediments contaminated by petroleum hydrocarbons.

et al. 2017

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ABSTRACT. Marine environments are a reservoir of relevant information on dangerous contaminants such as hydrocarbons, as well as microbial communities with probable degradation skills. However, to access microbial diversity, it is necessary to obtain high-quality DNA. An inexpensive, reliable, and effective metagenomic DNA (mgDNA) extraction protocol from marine sediments contaminated with petroleum hydrocarbons was established in this study from modifications to Zhou's protocol. The optimization included pretreatment of sediment with saline solutions for the removal of contaminants, a second precipitation and enzymatic degradation of RNA, followed by purification of mgDNA extracted by electroelution. The results obtained indicated that the modifications applied to 12 sediments with total petroleum hydrocarbon (TPH) concentrations from 22.6-174.3 (µg/g dry sediment) yielded 20.3-321.3 ng/µL mgDNA with A 260 /A 280 and A 260 /A 230 ratios of 1.75 ± 0.08 and 1.19 ± 0.22, respectively. The 16S rRNA amplification confirmed the purity of the mgDNA. The suitability of this mgDNA extraction protocol lies in the fact that all chemical solutions utilized are common in all molecular biology laboratories, and the use of dialysis membrane does not require any sophisticated or expensive equipment, only an electrophoretic chamber.

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Microbiological analysis of seawater and sediments in urban shorelines: Occurrence of heavy metals resistance bacteria on Chennai beaches, Bay of Bengal

Cited by 25 publications

References 21 publications

Biomonitoring along the Tropical Southern Indian Coast with Multiple Biomarkers

Biomonitoring along the Tropical Southern Indian Coast with Multiple Biomarkers

Removal of pathogenic bacteria from sewage-treated effluent and biosolids for agricultural purposes

Research Article Inexpensive metagenomic DNA extraction protocol with high quality from marine sediments contaminated by petroleum hydrocarbons.

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