2017
DOI: 10.4238/gmr16039743
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Research Article Inexpensive metagenomic DNA extraction protocol with high quality from marine sediments contaminated by petroleum hydrocarbons.

Abstract: ABSTRACT. Marine environments are a reservoir of relevant information on dangerous contaminants such as hydrocarbons, as well as microbial communities with probable degradation skills. However, to access microbial diversity, it is necessary to obtain high-quality DNA. An inexpensive, reliable, and effective metagenomic DNA (mgDNA) extraction protocol from marine sediments contaminated with petroleum hydrocarbons was established in this study from modifications to Zhou's protocol. The optimization included pret… Show more

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“…gDNA was extracted from 5 g of sediment following a standard extraction protocol ( Zhou, Bruns & Tiedje, 1996 ) modified by Osborn et al (2000) and García-Bautista et al (2017) . gDNA was precipitated with a solution of sodium acetate/isopropanol (0.3, 0.7 v/v) and washed with 70% ethanol.…”
Section: Methodsmentioning
confidence: 99%
“…gDNA was extracted from 5 g of sediment following a standard extraction protocol ( Zhou, Bruns & Tiedje, 1996 ) modified by Osborn et al (2000) and García-Bautista et al (2017) . gDNA was precipitated with a solution of sodium acetate/isopropanol (0.3, 0.7 v/v) and washed with 70% ethanol.…”
Section: Methodsmentioning
confidence: 99%