Microbiological Assay Methods for Vitamins

Baker, Herman; Sobotka, Harry

doi:10.1016/s0065-2423(08)60075-x

Cited by 35 publications

(7 citation statements)

References 174 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Baker and Sobotka (1962) mentioned 'low circulating levels of vitamin B6' in this condition (non-tropical sprue). The tryptophan loading test may also be abnormal in this condition (Kowlessar, Haeffner, and Benson, 1964), and evidence of malabsorption of B6 has been reported by Brain and Booth (1964) using tritiated pyridoxine.…”

Section: Control Subjectsmentioning

confidence: 93%

The measurement of serum pyridoxal by a microbiological assay usingLactobacillus casei

1970

View full text Add to dashboard Cite

SYNOPSIS A new method has been developed for the assay of serum pyridoxal using L. casei. Bound pyridoxal phosphate in serum is converted by acid hydrolysis to pyridoxal for which this organism is specific. This method proved to be considerably more sensitive than other methods so far reported in the literature.Serum pyridoxal concentrations were measured in 151 control subjects aged 17 to 80 years. The range of concentrations found was 1l5 to 13'5 ng/ml which compared well with values obtained by most workers measuring pyridoxal phosphate by enzymatic methods. A marked fall with age was confirmed, and levels in women of childbearing age were lower than in men of comparable age. Subnormal serum pyridoxal concentrations were found in 62 % of patients with sideroblastic anaemia and in the majority of patients with rheumatoid arthritis, Crohn's disease, coeliac disease, and in pregnant women at term.

show abstract

Section: Control Subjectsmentioning

confidence: 93%

The measurement of serum pyridoxal by a microbiological assay usingLactobacillus casei

1970

View full text Add to dashboard Cite

show abstract

“…The results in idiopathic steatorrhoea demonstrate the variability of absorption of different substances in different patients and provide a rational explanation for the very variable nutritional deficiences which may occur in this condition. Malabsorption of pyridoxine in these patients also explains why abnormalities of tryptophan metabolism (Kowlessar et al, 1961;Sigler et al, 1962) and subnormal serum pyridoxine levels (Baker and Sobotka, 1962) may occur in idiopathic steatorrhoea.…”

Section: -0mentioning

confidence: 99%

“…is rarely apparent and Girdwood (1956) was unable to demonstrate malabsorption of aneurine, riboflavine, or pyridoxine in 10 patients with impaired absorption of folic acid. Recently subclinical deficiency of pyridoxine in patients with tropical or non-tropical sprue has been suggested by reports that there is an increase in the urinary excretion of xanthurenic acid and other metabolites of 1-tryptophan after an oral dose of this amino-acid (Kowlessar, Haeffner, Benson, and Sleisenger, 1961;Sigler, Sheehy, Santini, and Rubini, 1962) and Baker and Sobotka (1962) have now shown that patients with nontropical sprue may have subnormal levels of pyridoxine in their serum. These observations suggest that there may be a failure of absorption of pyridoxine in idiopathic steatorrhoea.…”

mentioning

confidence: 99%

The absorption of tritium-labelled pyridoxine HCl in control subjects and in patients with intestinal malabsorption

Brain¹,

Booth²

1964

Gut

View full text Add to dashboard Cite

“…My colleague Dr. Herman Baker says it isn't alcohol as such, rather the "empty calories" represented by sucrose and ethanol that may make liver cirrhosis as often strike soft-drink addicts as alcoholics. Assessment of vitamin status and associated liver function will increasingly depend on protozoan assays for such indices as thiamine, nicotinic acid, vitamins B6 and BIZ, and biotin (7). Folic acid and riboflavin may be added when Tetrahymena assays are improved, likewise choline when the choline-requiring tetrahymenas discovered by Elliott et a1.…”

Section: Polycyclic Hydrocarbon Carcinogensmentioning

confidence: 99%

Protozoa as Toxicological Tools*

Hutner

1964

The Journal of Protozoology

View full text Add to dashboard Cite

At the age of 50 a Puka-Pukan retires from manual labor and becomes one of the members of The Company, spending his evenings shooting popguns or playing checkers, and his nights in ordering the young fry about. He never again has to go fishing, to gather coconuts, or to do any other work.When the malos [young men] bring in their fish they are given to the fathers, who divide them after picking out the choice bits for themselves. . . To be sure, many of the fathers spend a day or two at fishing now and then, but this is not required of them and they do it only to pass the time. . . ( 2 2 ) .HE experiments sketched here-many by my T younger colleagues-mold shotgun shells from the pharmacopoeia and pop them at protozoa in the hope that hits will reveal something about protozoan metabolism. The purpose is really phylogenetic: to trace forerunners of the coordination devices that made possible metazoa. Which of those devices originated in the protozoa? One assumes that every device for intercellular coordination began intracellularly. A chemotherapeutic agent that injures protozoa and metazoa but not bacteria hints at a protozoan origin for the metabolic device in question; likewise an antibiotic such as penicillin which kills bacteria but not protozoa or metazoa points to devices confined to bacteria-in the case of penicillin, a kind of cell wall seen only in bacteria and blue-green algae ( 1 1 ) .

show abstract

Microbiological Assay Methods for Vitamins

Cited by 35 publications

References 174 publications

The measurement of serum pyridoxal by a microbiological assay usingLactobacillus casei

The measurement of serum pyridoxal by a microbiological assay usingLactobacillus casei

The absorption of tritium-labelled pyridoxine HCl in control subjects and in patients with intestinal malabsorption

Protozoa as Toxicological Tools*

Contact Info

Product

Resources

About