Microbiological assessment of cadaver skin grafts received in a Skin Bank

Mathur, M; De, Anuradha; Gore, Madhuri

doi:10.1016/j.burns.2008.04.001

Cited by 28 publications

(21 citation statements)

References 5 publications

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“…Studies were all single-centered laboratory reports. Six studies were prospective (Baldeschi et al 1998; Kairiyama et al 2009; Lomas et al 2003; Rooney et al 2008; van Baare et al 1998; White et al 1991) and six were retrospective in design (Ireland and Spelman 2005; Lindford et al 2010; Mathur et al 2009; Neely et al 2008; Pianigiani et al 2010; Pirnay et al 2012). …”

Section: Resultsmentioning

confidence: 99%

“…The presence of microorganisms was confirmed by culturing of bacterial or fungal species (Online Resource 5). Ten studies reported that 75.0 % (1931/2575) of allograft samples were eligible for release and/or were culture negative for microbial contamination prior to additional disinfection (Baldeschi et al 1998; Ireland and Spelman 2005; Lindford et al 2010; Mathur et al 2009; Neely et al 2008; Pianigiani et al 2006; Pirnay et al 2012; Rooney et al 2008; van Baare et al 1998; White et al 1991). …”

Section: Resultsmentioning

confidence: 99%

“…In three studies, the tissue discard rate was reduced to an average of 19.1 % when penicillin and streptomycin were used in conjunction with the antifungal agent, amphotericin B or nystatin (Pianigiani et al 2010; Pirnay et al 2012; White et al 1991). The presence of the fungus, Candida, was observed in the initial bioburden following tissue recovery in five studies, suggesting their relatively consistent prevalence in skin allografts and the potential need to address these contaminating fungi when choosing a disinfection procedure (Baldeschi et al 1998; Ireland and Spelman 2005; Mathur et al 2009; Pianigiani et al 2010; Pirnay et al 2012). The clinical use of antibiotics and antifungals clinically to treat infections attests to their relative safety when being applied to tissue in relation to cellular integrity.…”

Section: Discussionmentioning

confidence: 99%

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Disinfection of human skin allografts in tissue banking: a systematic review report

Johnston¹,

Callum²,

Mohr

et al. 2016

Cell Tissue Bank

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The use of skin allografts to temporarily replace lost or damaged skin is practiced worldwide. Naturally occurring contamination can be present on skin or can be introduced at recovery or during processing. This contamination can pose a threat to allograft recipients. Bacterial culture and disinfection of allografts are mandated, but the specific practices and methodologies are not dictated by standards. A systematic review of literature from three databases found 12 research articles that evaluated bioburden reduction processes of skin grafts. The use of broad spectrum antibiotics and antifungal agents was the most frequently identified disinfection method reported demonstrating reductions in contamination rates. It was determined that the greatest reduction in the skin allograft contamination rates utilized 0.1 % peracetic acid or 25 kGy of gamma irradiation at lower temperatures.Electronic supplementary materialThe online version of this article (doi:10.1007/s10561-016-9569-2) contains supplementary material, which is available to authorized users.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Disinfection of human skin allografts in tissue banking: a systematic review report

Johnston¹,

Callum²,

Mohr

et al. 2016

Cell Tissue Bank

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“…The potential safety of xenogeneic tissue and products has been a subject of considerable debate, but evidence suggests that swine and humans share only a limited number of pathogens, reducing the risk of disease transmission [23,24]. Furthermore, swine for production of xenogeneic tissue can be maintained in climate controlled, pathogen-free environment, further reducing the risk of contaminated grafts in a manner not possible with allograft.…”

Section: Discussionmentioning

confidence: 99%

Skin grafts from genetically modified α-1,3-galactosyltransferase knockout miniature swine: A functional equivalent to allografts

Leonard

Mallard

Albritton

et al. 2017

Burns

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Burn is associated with a considerable burden of morbidity worldwide. Early excision of burned tissue and skin grafting of the resultant wound has been established as a mainstay of modern burn therapy. However, in large burns, donor sites for autologous skin may be limited. Numerous alternatives, from cadaver skin to synthetic substitutes have been described, each with varying benefits and limitations. We previously proposed the use of genetically modified (alpha-1,3-galactosyl transferase knockout, GalT-KO) porcine skin as a viable skin alternative. In contrast to wild type porcine skin, which has been used as a biologic dressing following glutaraldehyde fixation, GalT-KO porcine skin is a viable graft, which is not susceptible to loss by hyperacute rejection, and undergoes graft take and healing, prior to eventual rejection, comparable to cadaver allogeneic skin. In the current study we aimed to perform a detailed functional analysis of GalT-KO skin grafts in comparison to allogeneic grafts for temporary closure of full thickness wounds using our baboon dorsum wound model. Grafts were assessed by measurement of fluid loss, wound infection rate, and take, and healed appearance, of secondary autologous grafts following xenograft rejection. Comparison was also made between fresh and cryopreserved grafts. No statistically significant difference was identified between GalT-KO and allogeneic skin grafts in any of the assessed parameters, and graft take and function was not adversely effected by the freeze–thaw process. These data demonstrate that GalT-KO porcine grafts are functionally comparable to allogeneic skin grafts for temporary closure of full thickness wounds, and support their consideration as an alternative to cadaver allogeneic skin in the emergency management of large burns.

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“…As a biologic dressing CPA provides an adherent barrier which serves to reduce insensible fluid and heat losses, preventing wound bed desiccation, improving pain control, and providing a substrate for revascularization [4,[16][17][18]. Opponents to CPA use assert that cryopreservation, while preserving the biologic activity of skin, may also preserve bacterial [19] and viral [20] pathogens. However, several experimental studies have failed to demonstrate disease transmission [21,22].…”

Section: Introductionmentioning

confidence: 99%

Inability to determine tissue health is main indication of allograft use in intermediate extent burns

Fletcher¹,

Cancio

Sinha

et al. 2015

Burns

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Microbiological assessment of cadaver skin grafts received in a Skin Bank

Cited by 28 publications

References 5 publications

Disinfection of human skin allografts in tissue banking: a systematic review report

Disinfection of human skin allografts in tissue banking: a systematic review report

Skin grafts from genetically modified α-1,3-galactosyltransferase knockout miniature swine: A functional equivalent to allografts

Inability to determine tissue health is main indication of allograft use in intermediate extent burns

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