Microbiological Contamination of Laboratory Mice and Rats in Korea from 1999 to 2003

Won, Young‐Suk; Jeong, Eui‐Suk; Park, Hyun Ji; Lee, Chul‐Ho; Nam, Ki-Hoan; Kim, Hyoung-Chin; Hyun, Byung-Hwa; Lee, Sang-Koo; Choi, Yang Kyu

doi:10.1538/expanim.55.11

Cited by 26 publications

(41 citation statements)

References 11 publications

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“…Our data showed that 23.2% of tested facilities had MHV seropositive mouse, and MHV-infected mouse facilities with barrier systems account for 8.8% of the tested facilities. Compared to the monitoring data of 1999-2003 in Korea [19], which showed that 85% of conventional mouse facilities and 13.2% of barrier facilities were MHV positive, a decrease in MHV infection rates at both kinds of facility was observed in this study. MHV, classified as a category B pathogen, can be fatal to animals and is widespread in laboratory and wild mice [17].…”

Section: Discussioncontrasting

confidence: 62%

“…The most prevalent pathogen was MHV (Fig. 1), a very stubborn laboratory mouse pathogen in Korea which has been present for a long time [16,19]. Our data showed that 23.2% of tested facilities had MHV seropositive mouse, and MHV-infected mouse facilities with barrier systems account for 8.8% of the tested facilities.…”

Section: Discussionmentioning

confidence: 70%

“…Additional professional guidance is necessary to provide surveillance, to meet specific institutional needs, and to maximize the informational yield from the financial resources available for this purpose [18]. While a decrease in the infection rates of conventional animal facilities were observed in this study compared to the data from 1999 to 2003 [19], overall improvements in biosecurity including microbiological quality control and management are still needed. Although recommendations for health surveillance programs to protect and monitor the possible entry of pathogens are well documented, the actions to be taken when positive findings occur have yet to be established in institutional research centers in Korea.…”

Section: Discussionmentioning

confidence: 77%

“…Ten ICR mice purchased from each company were euthanized by carbon dioxide overexposure after blood collection under balanced anesthesia using ketamine and xylazine. Samples of blood, liver, kidney, spleen, lung, and colon feces were (Table 3) [5,11,19]. All animal experiments were performed under protocols approved by the Institutional Animal Care and Use Committee of Seoul National University.…”

Section: Animals and Specimen Collectionmentioning

confidence: 99%

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Microbiological Quality Assessment of Laboratory Mice in Korea and Recommendations for Quality Improvement

Seok

Lee

et al. 2010

Exp. Anim.

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Regular monitoring of commercial laboratory rodents and institutional research animal residents is essential for microbiological quality control programs. The objective of our study was to investigate the recent prevalence of infectious pathogens in laboratory mice from eight experimental animal vendors and 56 institutional animal facilities in Korea. Our investigation was conducted in 2006-2007. Specific Pathogen Free (SPF) mice from four commercial breeders were clean according to serological, bacteriological, parasitological, and histopathological examination results. However, mice from one intermediate vendor that distributed SPF animals from main commercial vendors to local districts had Syphacia obvelata and Mycoptes musculinus infections. Additionally, mice from conventional animal breeders were highly contaminated. Among the 56 institutional animal facilities, mouse hepatitis virus (MHV), Sendai virus and Mycoplasma pulmonis positive results were obtained in 23.2, 8.9, and 1.8% of animals tested, respectively. These results indicate that quarantine and eradication efforts of infectious pathogens in these facilities are sub-optimal and need to be improved. The use of commercial conventional mice for research should be eliminated and appropriate vendor selection as well as thorough quarantine before releasing animals into a facility are needed. Finally we suggest qualified veterinary experts are needed at each animal facility to ensure an adequate health surveillance program.

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Section: Discussioncontrasting

confidence: 62%

Section: Discussionmentioning

confidence: 70%

Section: Discussionmentioning

confidence: 77%

Section: Animals and Specimen Collectionmentioning

confidence: 99%

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Microbiological Quality Assessment of Laboratory Mice in Korea and Recommendations for Quality Improvement

Seok

Lee

et al. 2010

Exp. Anim.

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“…In conventional laboratory mouse and rat facilities in Japan, Staphylococcus aureus, intestinal protozoa, Pseudomonas aeruginosa, and M. pulmonis have been observed, while the most common pathogens in Japan are MHV and Pasteurella pneumotropica [15]. MHV and M. pulmonis are enzootic to Korea [26]. When selecting pathogens for the PEP, the current microbiological status in each country should be considered, and the common pathogens in each country should be included in test samples.…”

mentioning

confidence: 99%

First Trial in the Developmental Phase of the "Performance Evaluation Program" Based on the ICLAS Animal Quality Network Program: Self-Assessment of Microbiological Monitoring Methods Using Test Samples Supplied by ICLAS

et al. 2009

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Abstract:The first trial in the developmental phase of the "Performance Evaluation Program" based on the new programs of the International Council for Laboratory Animal Science (ICLAS) was carried out. ICLAS supplied test samples to each diagnostic laboratory for self-assessment of microbiological monitoring methods. We found that 1 mouse serum sample was positive for mouse minute virus and another was positive for Mycoplasma pulmonis antibodies, and 1 rat serum sample was positive for Sendai virus antibody. Mouse parvovirus was detected from mouse spleen and mesenteric lymph node homogenate, and Helicobacter spp. were detected from mouse feces. Corynebacterium bovis was isolated from a mouse skin sample. These results were in agreement with those notified by the ICLAS after the trial. After this trial, the program will eventually be made available to all diagnostic laboratories willing to participate in it.

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Health Monitoring Programmes and International Standards

Nicklas,

Buchheister,

Bleich

2024

Laboratory Animal Science and Medicine

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Microbiological Contamination of Laboratory Mice and Rats in Korea from 1999 to 2003

Cited by 26 publications

References 11 publications

Microbiological Quality Assessment of Laboratory Mice in Korea and Recommendations for Quality Improvement

Microbiological Quality Assessment of Laboratory Mice in Korea and Recommendations for Quality Improvement

First Trial in the Developmental Phase of the "Performance Evaluation Program" Based on the ICLAS Animal Quality Network Program: Self-Assessment of Microbiological Monitoring Methods Using Test Samples Supplied by ICLAS

Health Monitoring Programmes and International Standards

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