Microbiological diversity in the Unfinished Sacristy building of the Convent of Christ, Tomar, and evaluation of its biocide-based control

Abstract

Deterioração da pedra Alteração biológica Bactérias Fungos Biocidas Resumo Com o objetivo de caracterizar os microrganismos que colonizam as paredes calcárias da Sacristia Incompleta do Convento de Cristo, em Tomar, e estudar em laboratório o seu controlo por agentes biocidas, recolheram-se por swabbing amostras de seis locais e caracterizou-se a flora microbiana presente cultivável em laboratório, recorrendo a técnicas de microbiologia clássica. Verificou-se a presença de fungos, de bactérias heterotróficas e de microrganismos autotróficos em todas as amostras. Avaliou-se a eficácia relativa de diferentes biocidas através dos halos de inibição que provocam em placas de crescimento dos isolados bacterianos e fúngicos e testou-se o Biotin T, o cloreto de benzalcónio e, no caso dos fungos, também um aditivo para tintas. O cloreto de benzalcónio mostrou-se mais eficaz com os isolados bacterianos e o Biotin T com os isolados fúngicos.

Evaluation of the antifungal efficiency of biocides currently applied in the Coimbra UNESCO area limestone monuments

“…Moreover, Sanmartín and Carballeira [4], while working to evaluate the effects of similar biocidal treatments in heterotrophic microbial communities in the Monastery of San Martiño Pinario (Santiago de Compostela, Spain), also verified that Biotin T was the most effective treatment against fungal species. Additionally, Mateus et al [17] also observed a good Biotin T efficiency against fungi retrieved from limestone walls of the unfinished Sacristy of the Convent of Christ (Tomar, Portugal). Nonetheless, it is also noteworthy to mention that while working on churches in Segovia (Spain), de los Ríos et al [18] noticed that Biotin T application resulted in a cleaner stone surface, although unfortunately fungal hyphae were detected after two months of treatment application.…”

Chemical Characterization of Essential Oils With a Biocide Base for Conservation and Restoration

“…For instance, quaternary ammonium compounds, phenols, aldehydes, and alcohols are a clear example of disinfectants, with temporary results and long-term damage to the preserved artifacts [2,7,8]. Environmental awareness, side effects of disinfectants and inhibitors led to the need for new materials with biocidal effects.…”