Microbiological evaluation of anatomical organs submitted to glycerinization and freeze-drying techniques

Justo, Marianna; Cury, Fabio Sergio; Godoy, Silvia Helena Seraphin de; Fernandes, Andrezza Maria; Martins, Daniele dos Santos; Ambrósio, Carlos Eduardo

doi:10.1016/j.tria.2016.06.001

Cited by 1 publication

(3 citation statements)

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“…In general, the tomographic images of the frozen specimens showed a lighter color when compared to the images produced in the glycerinated cadavers, which presented more opaque anatomical structures with attenuation of the bone structures, which were a darker and denser color [18,19]. Methods of preserving corpses can influence the quality of tomographic images [15]. Glycerination can worsen the tomographic image by causing hyperdensity of soft tissues, which is a limitation of the technique in these conditions.…”

Section: Discussionmentioning

confidence: 97%

“…However, in the specimens preserved via glycerination, this method created topographic images without definitions and quality delineations of soft tissue, such as those that occur in bony structures, highlighting only the topographic localization of the stomach and part of the esophagus in the thoracic region, as can be seen in Figure 5. The delineation of the visualized structures probably occurred by changing density according to the preservation method [15].…”

Section: Discussionmentioning

confidence: 99%

“…The puppies were preserved using the glycerination technique [15]. The process took place in four stages, with the materials being immersed in different types of solutions and packed in plastic boxes at room temperature.…”

Section: Methodsmentioning

confidence: 99%

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Computed Tomography Evaluation of Frozen or Glycerinated Bradypus variegatus Cadavers: A Comprehensive View with Emphasis on Anatomical Aspects

Cunha,

Albuquerque,

Campos

et al. 2024

Animals

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Bradypus variegatus has unique anatomical characteristics, and many of its vascular and digestive tract aspects have yet to be clearly understood. This lack of information makes clinical diagnoses and surgical procedures difficult. The aim of this study was to evaluate the anatomical aspects of frozen and glycerinated corpses of B. variegatus using computed tomography (CT), emphasizing vascular and digestive contrast studies. Nine corpses that died during routine hospital were examined via CT in the supine position with scanning in the craniocaudal direction. In frozen cadavers, the contrast was injected into a cephalic vein after thawing and, subsequently, was administered orally. In addition to bone structures, CT allowed the identification of organs, soft tissues, and vascular structures in specimens. Visualization of soft tissues was better after contrast been administered intravenously and orally, even without active vascularization. Furthermore, the surfaces of the organs were highlighted by the glycerination method. With this technique, it was possible to describe part of the vascularization of the brachial, cervical, thoracic, and abdominal regions, in addition to highlighting the esophagus and part of the stomach. CT can be another tool for the evaluation of B. variegatus cadavers by anatomists or pathologists, contributing to the identification of anatomical structures.

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Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%