Microbiological Evaluation of Glycerolized Cadaveric Donor Skin

Baare, J. Van; Ligtvoet, E. E. J.; Middelkoop, Esther

doi:10.1097/00007890-199804150-00017

Cited by 63 publications

(31 citation statements)

References 19 publications

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“…All cells are nonviable but the collagen and elastin fibers are well preserved using this method (Richters et al 1996). In addition, glycerol 85% is a slow but effective in-activator of microorganisms (van Baare et al 1994(van Baare et al , 1998a.…”

Section: Introductionmentioning

confidence: 99%

Development of a dermal matrix from glycerol preserved allogeneic skin

et al. 2008

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Dermal substitutes can be used to improve the wound healing of deep burns when placed underneath expanded, thin autologous skin grafts. Such dermal matrix material can be derived from xenogeneic or human tissue. Antigenic structures, such as cells and hairs must be removed to avoid adverse inflammatory response after implantation. In this study, a cost-effective method using low concentrations of NaOH for the de-cellularization of human donor skin preserved in 85% glycerol is described. The donor skin was incubated into NaOH for different time periods; 2, 4, 6 or 8 weeks. These dermal matrix prototypes were analyzed using standard histology techniques. Functional tests were performed in a rat subcutaneous implant model and in a porcine transplantation model; the prototypes were placed in full thickness excision wounds covered with autologous skin grafts.An incubation period of 6 weeks was most optimal, longer periods caused damage to the collagen fibers. Elastin fibers were well preserved. All prototypes showed intact biocompatibility in the rat model by the presence of ingrowing blood vessels and fibroblasts at 4 weeks after implantation. An inflammatory response was observed in the prototypes that were treated for only 2 or 4 weeks with NaOH. The prototypes treated with 6 or 8 weeks NaOH were capable to reduce wound contraction in the porcine model. In neo-dermis of these wounds, elastin fibers derived from the prototype could be observed at 8 weeks after operation, surrounded by more random orientated collagen fibers. Thus, using this effective low cost method, a dermal matrix can be obtained from human donor skin. Further clinical studies will be performed to test this material for dermal substitution in deep (burn) wounds.

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Section: Introductionmentioning

confidence: 99%

Development of a dermal matrix from glycerol preserved allogeneic skin

et al. 2008

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“…The results showed that fresh skin in general has an antimicrobial effect against E. coli, S. aureus, but it does not necessary means CPA and GPA also have an antimicrobial effect. According to one study, 85% GPA has a strong antimicrobial effect [21]. But the results of this study showed CPA has a mild antimicrobial effect against 6 types of bacterial species.…”

Section: Discussionmentioning

confidence: 60%

Antimicrobial Effect of Skin for Allograft and Management in Burn Wound

Lim¹,

Yoon²,

Lee³

2017

OJOTS

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One of the most important functions of skins is to protect our bodies from microbes or pollutant sources. Skins containing physical substances serve as a physical barrier which protects our bodies from pathogens. A healthy skin contains a variety of antibacterial substances such as defensin, cathelicidin and psoriasin. However deep and wide burns cause the skin to lose its original functions, so our skins are exposed to various danger factors. For the burn patients, human alloskin graft serves as a very important temporary biological wound dressing. It protects the wound before autograft procedure, forms revascularization and granulation tissues and protects the wound from an invasion of microbes. This study was conducted with the aim to analyze the antimicrobial effect of cryopreserved allograft (CPA) and glycerol-preserved allograft (GPA) which was a type of allograft widely used for burn patients, and measure the difference in comparison with the fresh skin before processing it.The most common contaminants found in burn patients such as S. aureus, P. aeruginosa, C. albicans and E. coli, were used for experiment. The antimicrobial effect against S. aureus and E. coli was observed in fresh skin and some CPA. In some clinical cases, infection is frequently observed in the wounds treated with allograft, indicating the allograft completely block every kind of microbes. To prevent the infection, it is required to use antibiotics and manage wounds thoroughly.

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“…We set out to develop a dermal substitute from glycerol preserved allografts more than a decade ago, which was intended to have the following key advantages: native collagen and elastin matrix, easy storage and handling, inactivation of virus and micro-organisms [45,46] and most importantly, a non-profit product that could be available to a larger number of patients. The extreme high cost of dermal substitutes today impedes their widespread application and benefit for those who need it the most.…”

Section: Discussionmentioning

confidence: 99%