Microbiological findings of infected root canals and adjacent periodontal pockets in teeth with advanced periodontitis

Kipioti, A; Nakou, Mela; Legakis, N. J.; Mitsis, F

doi:10.1016/0030-4220(84)90139-7

Cited by 72 publications

(87 citation statements)

References 39 publications

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“…[96][97][98][99][100][101] Bacteria may enter the tooth via caries, 102-105 dental anomalies (e.g., dens invaginatus, deep lingual and palatal grooves), exposed lateral canals or damaged cementum as a result of periodontal diseases, 106,107 tooth cracks or fractures, 108,109 and marginal breakdown at the restoration-tooth interface. [110][111][112][113] Bacterial infections of the pulp space consist of mixed microbial and predominantly anaerobic flora.…”

Section: Bacteriamentioning

confidence: 99%

An overview of the dental pulp: its functions and responses to injury

Yu¹,

Abbott²

2007

Australian Dental Journal

238

158

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The dental pulp is a unique tissue and its importance in the long-term prognosis of the tooth is often ignored by clinicians. It is unique in that it resides in a rigid chamber which provides strong mechanical support and protection from the microbial rich oral environment. If this rigid shell loses its structural integrity, the pulp is under the threat of the adverse stimuli from the mouth, such as caries, cracks, fractures and open restoration margins, all of which provide pathways for micro-organisms and their toxins to enter the pulp. The pulp initially responds to irritation by becoming inflamed and, if left untreated, this will progress to pulp necrosis and infection. The inflammation will also spread to the surrounding alveolar bone and cause periapical pathosis. The magnitude of pulp-related problems should not be underestimated since their most serious consequence is oral sepsis, which can be life threatening, and hence correct diagnosis and management are essential. Clinicians must have a thorough understanding of the physiological and pathological features of the dental pulp as well as the biological consequences of treatment interventions.

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Section: Bacteriamentioning

confidence: 99%

An overview of the dental pulp: its functions and responses to injury

Yu¹,

Abbott²

2007

Australian Dental Journal

238

158

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“…However, the primary cause of both diseases is the presence of bacterial infection [1][2][3][4][5]. Studies suggest that infected root canal systems and periodontal pockets have similar microbiological flora [2,[4][5][6][7][8] and it is widely accepted that untreated infections of one of these tissues can lead to signs or symptoms of disease initiation and progression within the other compartment [6,7,9,10]. This can occur in either direction, for example from the root canal to the periodontium and vice versa.…”

Section: Introductionmentioning

confidence: 99%

Endodontic drug delivery for root surface disinfection: a laboratory feasibility evaluation

et al. 2015

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OBJECTIVES This study aims to assess the potential of a mixture of three antibiotics (TreVitaMix, TVM) as an intracanal dressing to disinfect the outer root surface by applying a new in vitro model. MATERIALS AND METHODS Fifty freshly extracted bovine roots were endodontically treated. Forty samples were then thoroughly scaled, mounted to petri dishes, gas sterilized, and randomly allocated to four groups (n = 10/group) according to their intracanal medication: sterile saline (NaCl; control, A); the TVM carrier material alone, i.e., propylene glycol (PG; B); TVM (C); and calcium hydroxide (D). In an additional group (E), the cementum was not removed and TVM was placed. Petri dishes were filled with Fastidious Anaerobe Agar, inoculated with Fusobacterium nucleatum suspension and then anaerobically incubated during 48-h intervals at 37°C up to 192 h. Inhibition zones around the roots were then measured after each incubation period (mm(2)). RESULTS Only teeth inoculated with the TVM dressing showed inhibition at all time points, whereas the other treatments showed no peri-radicular growing inhibition. Presence of cementum had no negative effect on disinfection (p = 0.9320). CONCLUSION TVM was able to penetrate through the dentine and inhibit the bacterial growth of F. nucleatum up to 192 h. CLINICAL RELEVANCE TVM might have the potential to sustainably disinfect the outer root surface in perio-endo lesions and serve as an adjunctive antimicrobial agent. Declaration of interests:The authors declare they have no conflicts of interest. AbstractObjectives To assess the potential of a mixture of three antibiotics (TreVitaMix, TVM) as an intracanal dressing to disinfect the outer root surface applying a new in vitro model. Materials and MethodsFifty freshly extracted bovine roots were endodontically treated. Forty samples were then thoroughly scaled, mounted to petri dishes, gas sterilized and randomly allocated to four groups (n=10/group) according their intracanal medication: Sterile saline NaCl (control, A), the TVM carrier material alone, i.e. propylene glycol (PG; B), TVM (C) and calcium hydroxide (D). In an additional group (E), the cementum was not removed and TVM was placed. Petri dishes were filled with Fastidiuos Anaerobe Agar, inoculated with F. nucleatum suspension and then anaerobically incubated during 48 h intervals at 37°C up to 192 h. Inhibition zones around the roots were then measured after each incubation period (mm 2 ).Results Only teeth inoculated with the TVM dressing showed inhibition at all time points, whereas the other treatments showed no peri-radicular growing inhibition. Presence of cementum had no negative effect on the disinfection (p = 0.9320).Conclusion TVM was able to penetrate through dentine and inhibit the bacterial growth of F. nucleatum up to 192 h.Clinical relevance TVM might have the potential to sustainably disinfect the outer root surface in perio-endo lesions and serve as an adjunctive antimicrobial agent.

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“…Since the first report by Simring and Goldberg 9) in 1964, several studies have investigated the relationship between the microflora in endodontic-periodontal lesions and primary periodontal disease [3][4][5][6]8) . Kobayashi et al 5) reported that microorganisms common to root canals and periodontal pockets were detected in caries-free teeth with advanced periodontitis.…”

Section: Fusobacterium Nucleatum Parvimonas Micra (Peptostreptococcusmentioning

confidence: 99%

“…Endodontic-periodontal lesions are clinically classified into three categories based on their site of origin 8) : (a) primary endodontic lesions; (b) primary peri-odontal lesions; and (c) "true" combined lesions. Disease transmission between these first two types of lesion has been demonstrated in several microbiological studies, which revealed similarities in the bacterial profiles of infected root canals and advanced periodontitis [3][4][5]11) . However, the microbiota that actually causes such lesions has not been fully investigated.…”

Section: Introductionmentioning

confidence: 99%

An Endodontic-periodontal Lesion with Primary Periodontal Disease: A Case Report on Its Bacterial Profile

Fujii

Muramatsu

Yamaguchi

et al. 2014

Bull. Tokyo Dent. Coll.

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The microflora in the periodontal pockets can affect the dental pulp and cause endodontic-periodontal lesions or retrograde pulpitis. Here we report an endodonticperiodontal lesion together with its bacterial profile. The lesion occurred in the maxillary right first molar of a 40-year-old woman who presented at our hospital complaining of a violent toothache since the previous night. Clinically, the tooth was caries-free and an electric pulp test showed it to be vital. The tooth showed signs of advanced periodontitis and the periodontal pocket was deep, reaching the apex of the palatal root. The clinical diagnosis was an endodontic-periodontal lesion with primary periodontal disease. Subsequent endodontic treatment comprised pulp extirpation and root canal filling, followed by periodontal treatment consisting of scaling and root planing. The tooth was finally restored with a full metal crown. No further signs of periodontal disease or periapical lesions have been observed to date. Bacteria were sampled from the root canal and periodontal pocket for a microbiological assessment using 16S rRNA gene-based PCR. Microbiologically, the profile of the bacterial species from the palatal root canal was similar to that from the periodontal pocket of the palatal root. Porphyromonas gingivalis, Fusobacterium nucleatum and Eikenella corrodens were detected in both samples. The occurrence of bacteria common to both sites in this patient further supports the proposition that periodontal disease is the definitive source of root canal infections. The present results suggest that a bacterial examination would be helpful in confirming and supporting the clinical diagnosis in such lesions.

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Microbiological findings of infected root canals and adjacent periodontal pockets in teeth with advanced periodontitis

Cited by 72 publications

References 39 publications

An overview of the dental pulp: its functions and responses to injury

An overview of the dental pulp: its functions and responses to injury

Endodontic drug delivery for root surface disinfection: a laboratory feasibility evaluation

An Endodontic-periodontal Lesion with Primary Periodontal Disease: A Case Report on Its Bacterial Profile

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