PURPOSE: Evaluating the effect of a proximal margin elevation technique on marginal adaptation of ceramic inlays. METHODS: Class II MOD-cavities were prepared in 40 human molars and randomly distributed to four groups (n = 10). In group EN (positive control) proximal margins were located in enamel, 1 mm above the cementoenamel junction, while 2 mm below in groups DE-1In, DE-2In and DE. The groups DE-1In, DE-2In and DE simulated subgingival location of the cervical margin. In group DE-1In one 3 mm and in group DE-2In two 1.5 mm composite layers (Tetric) were placed for margin elevation of the proximal cavities using Syntac classic as an adhesive. The proximal cavities of group DE remained untreated and served as a negative control. In all groups, ceramic inlays (Cerec 3D) were adhesively inserted. Replicas were taken before and after thermomechanical loading (1.200.000 cycles, 50/5°C, max. load 49 N). Marginal integrity (tooth-composite, composite-inlay) was evaluated with scanning electron microscopy (200×). Percentage of continuous margin (% of total proximal margin length) was compared between groups before and after cycling using ANOVA and Scheffé post-hoc test. RESULTS: After thermomechanical loading, no significant differences were observed between the different groups with respect to the interface composite-inlay and tooth-composite with margins in dentin. The interface tooth-composite in enamel of group EN was significantly better compared to group DE-2In, which was not different to the negative control group DE and DE-1In. CONCLUSION: Margin elevation technique by placement of a composite filling in the proximal box before insertion of a ceramic inlay results in marginal integrities not different from margins of ceramic inlays placed in dentin. Influence of a proximal margin elevation technique on marginal adaptation of ceramic inlaysZaruba, M; Göhring, T N; Wegehaupt, F J; Attin, T Abstract: PURPOSE: Evaluating the effect of a proximal margin elevation technique on marginal adaptation of ceramic inlays. METHODS: Class II MOD-cavities were prepared in 40 human molars and randomly distributed to four groups (n = 10). In group EN (positive control) proximal margins were located in enamel, 1 mm above the cementoenamel junction, while 2 mm below in groups DE-1In, DE-2In and DE. The groups DE-1In, DE-2In and DE simulated subgingival location of the cervical margin. In group DE-1In one 3 mm and in group DE-2In two 1.5 mm composite layers (Tetric) were placed for margin elevation of the proximal cavities using Syntac classic as an adhesive. The proximal cavities of group DE remained untreated and served as a negative control. In all groups, ceramic inlays (Cerec 3D) were adhesively inserted. Replicas were taken before and after thermomechanical loading (1.200.000 cycles, 50/5°C, max. load 49 N). Marginal integrity (tooth-composite, composite-inlay) was evaluated with scanning electron microscopy (200×). Percentage of continuous margin (% of total proximal margin length) was compared between groups be...

Matrix metalloproteinase (MMP) inhibitors prevented bond strength loss of an etch-andrinse adhesive over time. Bond strength of a self-etching adhesive was not significantly reduced with 9 months of aging; thus, MMP inhibitors did not show a significant effect. SUMMARY Aim:This study aimed to analyze the effect of different matrix metalloproteinase (MMP) inhibitors on the microtensile bond strength (microTBS) of an etch-and-rinse and a selfetching adhesive after 9 months of aging.Methods and Materials: Flat human dentin surfaces were bonded either with an etch-andrinse adhesive (Optibond FL) or a self-etching adhesive (Clearfil SE Bond). Dentin surfaces were left untreated or were pretreated with MMP inhibitors (2% chlorhexidine digluconate [CHX], 0.05% green tea extract, 1 mM ferrous sulfate, or 0.2 mM galardin) prior to application of the adhesive. Composite buildups were made incrementally. Pretreated groups were tested after 9 months of storage in artificial saliva (378C) and compared with untreated groups, which were tested immediately (initial microTBS) and upon aging (9-month microTBS). Data were analyzed by linear mixedmodel regression. Failure mode analysis was performed microscopically and statistically analyzed by repeated-measures analysis of variance (p,0.05).

The fracture behavior of CAD/CAM fabricated crowns was investigated as a function of material thickness for six silicate ceramic materials: Mark II, e.max CAD, Celtra Duo milled, Celtra Duo fired, Suprinity, Enamic. Crowns with thicknesses 0.5/1.0/1.5 mm were fabricated with CEREC and adhesively seated to dies fabricated with stereolithography technology (n=144). Thermomechanical loading and fractural loading was performed. Statistical analysis was done with one-way ANOVA and post-hoc Scheffé test. For 1.5 mm, all crowns survived fatigue testing, for 1.0 mm, survival was 100% only for materials e.max CAD and Suprinity. For 0.5 mm, best survival rate was 37.5% for Suprinity. Maximum fractural loading significantly varied among the groups. For 0.5 mm, highest value was found for Suprinity (660.1 N). Mark II showed lowest values for 1.0 mm (482.0 N), and 1.5 mm groups (634.8 N). e.max CAD showed highest values for 1.0 mm (774.2 N), and 1.5 mm groups (1,240.8 N).Keywords: Feldspathic ceramic VITA Mark II, Lithium disilicate ceramic e.max CAD, Zirconia-reinforced lithium silicate ceramic Celtra Duo and VITA Suprinity, Hybrid ceramic VITA Enamic, CERECColor figures can be viewed in the online issue, which is available at J-STAGE.

OBJECTIVES: This study aims to evaluate the effect of a minimally invasive mesial-occlusaldistal (mod) preparation on the marginal adaptation of ceramic and composite inlays with the aim of saving sound dental substance. MATERIALS AND METHODS: Class II mod cavities were prepared in 50 extracted human molars and randomly allocated to five groups (n = 10). In all groups, the mesialproximal box margins were located in the dentin, 1 mm below the cementoenamel junction (CEJ), while the distal box margins were 1 mm above the CEJ. In groups A and B, conventional standard preparations with a divergent angle of = 6°were prepared. In groups C, D, and E, minimally invasive standard preparations with a convergent angle of = 10°were prepared. In groups A and D, composite inlays and, in groups B and C, ceramic inlays were fabricated (chairside economical restoration of esthetic ceramics (CEREC)) and adhesively inserted. In group E, a direct composite filling using the incremental technique was placed. Replicas were taken before and after thermomechanical loading (1,200,000 cycles, 50/5°C, max. load 49 N). Marginal integrity (tooth-luting composite, luting composite-inlay) was evaluated by scanning electron microscopy (×200). The percentage of continuous margins in the different locations was compared between and within groups before and after cycling, using ANOVA and Scheffé post hoc test. RESULTS: After the thermomechanical loading, no significant differences were observed between the different groups with respect to the interface of luting composite-inlay. At the interface of toothluting composite for preparations involving the dentin, groups A and B behaved significantly better compared to the control group E, which in turn were not different to groups C and D. CONCLUSION: Composite and ceramic inlays inserted in minimally invasive prepared mod cavities result in margins not different from those of inlays placed in conventional mod preparations. Direct composite filling margins, however, were inferior to those attained by conventional indirect restorations. CLINICAL RELEVANCE: Minimally invasive preparations for mod inlays with undercuts show marginal adaptation equal to that of conventional inlay preparation design. Keywords: Composite inlay, ceramic inlay; minimally-invasive; marginal adaption Declaration of interests:The authors declare they have no conflicts of interest. AbstractObjectives To evaluate the effect of a minimally-invasive mod-preparation on the marginal adaptation of ceramic and composite inlays with the aim of saving sound dental substance. Materials and MethodsClass II mod-cavities were prepared in 50 extracted human molars and randomly allocated to five groups (n=10). In all groups, the mesial-proximal-box-margins were located in dentin, 1 mm below the cementoenamel junction (CEJ), while the distal-boxmargins were 1 mm above the CEJ. In group A and B, conventional standard preparations with a divergent angle of α = 6° were prepared. In Group C, D and E minimally invasive standard preparation...

This study aimed to analyse the erosion-inhibiting potential of a single application of stannous chloride-containing fluoride solution on pellicle-covered enamel and dentine under constant acid flow conditions in vitro. DESIGN: Bovine enamel (n=60) and dentine (n=60) samples were exposed 1h to the oral cavity of 4 healthy volunteers to allow for in situ pellicle formation. Pellicle-covered samples were randomly assigned to three groups (each n=20 enamel and n=20 dentine samples; 5 enamel and 5 dentine samples/volunteer) and treated once with a SnCl2/AmF/NaF (800 ppm Sn(II), 500 ppm F, pH 4.5) or a NaF solution (500 ppm F, pH 4.5) for 2 min or remained untreated (controls). Samples were eroded with hydrochloric acid (pH 2.6) in a small erosion chamber at 60 microl/min for 25 min. Calcium release into the acid was monitored in consecutive 30s intervals for 5 min, then at 1 min intervals up to a total erosion time of 25 min using the Arsenazo III procedure. Data were statistically analysed by random-effects linear models (p<0.05). RESULTS: The stannous chloride-containing fluoride solution reduced calcium loss of enamel and dentine to up to 6 min and 3.5 min, respectively. Calcium loss (% of control) amounted from 24+/-7 (30s) up to 93+/-14 (6 min) in enamel and from 38+/-13 (30s) to 87+/-15 (3.5 min) in dentine. The sodium fluoride solution was unable to reduce enamel and dentine erosion at any time point. CONCLUSION: A single application of a stannous chloride-containing fluoride solution reduced enamel and dentine erosion up to 6 min and 3.5 min of constant acid flow, respectively. Design: Bovine enamel (n = 60) and dentine (n = 60) samples were exposed 1 h to the oral cavity of four healthy volunteers to allow for in situ pellicle formation. Pellicle-covered samples were randomly assigned to three groups (each n = 20 enamel and n = 20 dentine samples; 5 enamel and 5 dentine samples/ volunteer) and treated once with a SnCl 2 /AmF/NaF (800 ppm Sn(II), 500 ppm F, pH 4.5) or a NaF solution (500 ppm F, pH 4.5)for 2 min or remained untreated (controls). Samples were eroded with hydrochloric acid (pH 2.6) in a small erosion chamber at 60 µl/min for 25 min. Calcium release into the acid was monitored in consecutive 30 s intervals for 5 min, then at 1 min intervals up to a total erosion time of 25 min using the Arsenazo III procedure. Data were statistically analysed by randomeffects linear models (p < 0.05). Results:The stannous chloride-containing fluoride solution reduced calcium loss of enamel and dentine to up to 6 min and 3.5 min, respectively. Calcium loss (% of control) amounted from 24±7 (30 s) up to 93±14 (6 min) in enamel and from 38±13 (30 s) to 87±15 (3.5 min) in dentine. The sodium fluoride solution was unable to reduce enamel and dentine erosion at any time point. Conclusion:A single application of a stannous chloride-containing fluoride solution reduced enamel and dentine erosion up to 6 min and 3.5 min of constant acid flow, respectively.3