Microbiological profile of diabetic foot ulcers in Kuwait

Alhubail, Asma; Sewify, May; Messenger, Grace; Masoetsa, Richard; Hussain, Imtiaz; Nair, Shinu; Tiss, Ali

doi:10.1371/journal.pone.0244306

Cited by 25 publications

(27 citation statements)

References 38 publications

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“…In the EURODIALE study, male sex was found to be an independent predictor of non-healing DFU [ 38 ]. Similar findings have been reported in other populations [ 39 , 40 ]. Therefore, this could be partially explained by the hypothesis that prolonged non-healing DFU, which is more common in male patients, is likely to be the point of pathogen entry and consequently cause infections [ 28 , 41 ].…”

Section: Discussionsupporting

confidence: 92%

Classification Model for Diabetic Foot, Necrotizing Fasciitis, and Osteomyelitis

Kim

Yoo

Lee

et al. 2022

Biology

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Diabetic foot ulcers (DFUs) and their life-threatening complications, such as necrotizing fasciitis (NF) and osteomyelitis (OM), increase the healthcare cost, morbidity and mortality in patients with diabetes mellitus. While the early recognition of these complications could improve the clinical outcome of diabetic patients, it is not straightforward to achieve in the usual clinical settings. In this study, we proposed a classification model for diabetic foot, NF and OM. To select features for the classification model, multidisciplinary teams were organized and data were collected based on a literature search and automatic platform. A dataset of 1581 patients (728 diabetic foot, 76 NF, and 777 OM) was divided into training and validation datasets at a ratio of 7:3 to be analyzed. The final prediction models based on training dataset exhibited areas under the receiver operating curve (AUC) of the 0.80 and 0.73 for NF model and OM model, respectively, in validation sets. In conclusion, our classification models for NF and OM showed remarkable discriminatory power and easy applicability in patients with DFU.

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Section: Discussionsupporting

confidence: 92%

Classification Model for Diabetic Foot, Necrotizing Fasciitis, and Osteomyelitis

Kim

Yoo

Lee

et al. 2022

Biology

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“…In October 2021, we performed skin microbiota 16S rRNA gene high-throughput sequencing to determine bacterial infection after probiotic treatment. We used the Levine cotton swab method ( 54 ) to collect skin microbiota samples from crocodile lizards from each group. For sequencing, we constructed a 20-μl reaction system using TransGen AP221-02: TranStart Fastpfu Polymerase (TransGen Biotech, Beijing, China), including 5 × FastPfu Buffer (4 μl), 2.5 mM dNTPs (2 μl), forward primer (5 μM, 0.8 μl), reverse primer (5 μM, 0.8 μl), FastPfu Polymerase (0.4 μl), bovine serum albumin (BSA, 0.2 μl), template DNA (10 ng), and distilled water (to volume).…”

Section: Methodsmentioning

confidence: 99%

Identification of Pseudomonas aeruginosa From the Skin Ulcer Disease of Crocodile Lizards (Shinisaurus crocodilurus) and Probiotics as the Control Measure

Xiong

Qin

et al. 2022

Front. Vet. Sci.

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The crocodile lizard (Shinisaurus crocodilurus) is an endangered ancient reptile species. Captive breeding is an important conservation measure for the potential restoration and recovery of their wild populations. However, a skin ulcer disease caused by an unknown pathogen has become a serious threat to captive breeding individuals. In the current study, based on microbial isolation, we identified Pseudomonas aeruginosa as the dominant pathogen in skin ulcer disease. Chinese skinks (Plestiodon chinensis) were used to verify the pathogenicity of P. aeruginosa in skin ulcer disease in vivo. As expected, subcutaneous inoculation of P. aeruginosa induced skin disease in healthy skinks and P. aeruginosa was re-isolated from the induced skin ulcers. Therefore, P. aeruginosa, an opportunistic and ubiquitous pathogen that causes a wide range of infections, appears to be the main pathogen of the skin disease affecting crocodile lizards. In the aquaculture industry, probiotics are widely used in the prevention and control of animal diseases caused by such pathogens. Here, we administered probiotics to the breeding crocodile lizards for 6 months. The three experiment groups treated with different kinds of probiotics showed significance at controlling case incidence. Three of the four groups treated with probiotics showed significant disease prevention (Effective Microorganisms mixed probiotics P = 0.0374; Double-dose Effective Microorganisms, P = 0.0299; Bacillus subtilis, P = 0.0140, T-test), and CFUs in the water of the breeding enclosures were also inhibited after probiotics usage (P < 0.001, T-test). Our study demonstrated the role of Pseudomonas aeruginosa in development of skin ulcer disease of crocodile lizards in a local zoo and offered the probiotic-based method for control measurements, which would be of benefit for the conservation of endangered reptiles.

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“…Gram-positive bacteria predominate in ulcers free of ischemia, while Gram-negative bacteria predominate in ulcers affected by ischemia. Pseudomonas aeruginosa was more frequent in ulcers with infection and ischemia than Staphylococcus aureus was in ulcers without ischemia [ 116 ]. The microbiological profile of DFU in Lebanon is similar to that of other nations in the Middle East and North Africa (MENA) area, with significant variances when compared with that of the Western world.…”

Section: Recent Upgrade In the Field Of Dfusmentioning

confidence: 99%

An Overview of Diabetic Foot Ulcers and Associated Problems with Special Emphasis on Treatments with Antimicrobials

Baig¹,

Banu

Zehravi

et al. 2022

Life

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One of the most significant challenges of diabetes health care is diabetic foot ulcers (DFU). DFUs are more challenging to cure, and this is particularly true for people who already have a compromised immune system. Pathogenic bacteria and fungi are becoming more resistant to antibiotics, so they may be unable to fight microbial infections at the wound site with the antibiotics we have now. This article discusses the dressings, topical antibacterial treatment, medications and debridement techniques used for DFU and provides a deep discussion of DFU and its associated problems. English-language publications on DFU were gathered from many different databases, such as Scopus, Web of Science, Science Direct, Springer Nature, and Google Scholar. For the treatment of DFU, a multidisciplinary approach involving the use of diagnostic equipment, skills, and experience is required. Preventing amputations starts with patient education and the implementation of new categorization systems. The microbiota involved in DFU can be better understood using novel diagnostic techniques, such as the 16S-ribosomal DNA sequence in bacteria. This could be achieved by using new biological and molecular treatments that have been shown to help prevent infections, to control local inflammation, and to improve the healing process.

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Microbiological profile of diabetic foot ulcers in Kuwait

Cited by 25 publications

References 38 publications

Classification Model for Diabetic Foot, Necrotizing Fasciitis, and Osteomyelitis

Classification Model for Diabetic Foot, Necrotizing Fasciitis, and Osteomyelitis

Identification of Pseudomonas aeruginosa From the Skin Ulcer Disease of Crocodile Lizards (Shinisaurus crocodilurus) and Probiotics as the Control Measure

An Overview of Diabetic Foot Ulcers and Associated Problems with Special Emphasis on Treatments with Antimicrobials

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