Microbiological quality, chemical profile as well as antioxidant and antidiabetic activities of Schinus terebinthifolius Raddi

Abstract

Schinus terebinthifolius Raddi, commonly known as Brazilian peppertree, is a plant species widely used in Brazilian traditional medicine for various purposes. The objective of this study was to assess the microbiological quality, safety, chemical profile as well as antioxidant and antidiabetic potentials of different parts of S. terebinthifolius. Microbiological analysis of the methanolic extracts of the roots (MESR), stem bark (MESB) and leaves (MESL) of S. terebinthifolius showed no microbial growth. The concentrations of phenolic compounds, phenolic acids and flavonoids were determined by spectrophotometry. The phenolic compounds of the MESL were identified by liquid chromatography coupled to a diode array detector and mass spectrometer (LC-DAD-MS). The antioxidant activities of the extracts were analyzed by 2,2-diphenyl-1-(2,4,6-trinitrophenyl)hydrazyl radical (DPPH%), 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) radical (ABTS% +), fluorescence recovery after photobleaching (FRAP), reducing power, β-carotene bleaching and malondialdehyde (MDA) assays in human erythrocytes. The antidiabetic properties of the extracts were demonstrated in vitro by their inhibition of the α-glucosidase enzyme and their anti-glycation activity via fructose and glyoxal. After showing no acute toxicity in vivo, MESL was able to lower postprandial glycemia after glucose overload in normoglycemic mice as well as the water and feed intake, liver weight, glycemia and serum levels of glycated hemoglobin, aspartate transaminase (AST) and alanine transaminase (ALT) in diabetic mice. Overall, S. terebinthifolius extracts showed microbiological safety along with antioxidant and antidiabetic activities, likely mediated by its chemical constituents, such as gallic acid, gallotannins and glycosylated flavonols.

Acrocomia aculeata(Jacq.) Lodd. ex Mart. Leaves Increase SIRT1 Levels and Improve Stress Resistance

“…In this way, the protection against oxidation is considered beneficial for the maintenance of health and prevention against the development of several diseases [53]. Similar results were also obtained in Cerrado plants [36,63]. The prooxidant effect showed by EE-Aa and EM-Aa in high concentrations is probably caused by the generation of free radicals, same as reported with AA [64].…”

Schinus terebinthifoliusEssential Oil Attenuates Scopolamine-Induced Memory Deficits via Cholinergic Modulation and Antioxidant Properties in a Zebrafish Model

“…Supporting evidence suggested various biological effects of Schinus terebinthifolius Raddi. dos Santos da Rocha et al [24] reported that Schinus terebinthifolius extract exhibited antioxidant and antidiabetic profile in mice mediated by its compounds such as gallic acid, gallotannins, and glycosylated flavonols. Miller et al [25] demonstrated the vasodilatory activity of Schinus terebinthifolius extract in rats and mice and also antioxidant activities, especially in the CNS.…”

Caatinga: barn of antioxidant and nutraceutical bio-actives

“…The methanolic extract of the leaves of S. terebinthifolius obtained high concentrations of antioxidant compounds, evaluated by DPPH assay, in an animal model; results point to reducing oxidative stress and inhibiting cardiotoxicity induced by the drug doxorubicin (P. D. S. da Rocha et al, 2020b). In another study, the leaves methanolic extracts inhibited the action of α-glucosidase, an enzyme responsible for the rapid breakdown of polysaccharides in the intestinal wall and consequently its rapid ingestion (dos Santos da Rocha et al, 2019a). The extract also had greater functionality with the reference drug for the treatment of diabetes, metformin, at the concentrations tested in the work; with reduced postprandial glycemia after glucose overload in diabetic mice, decreased liver weight, blood glucose, and reduced serum glycated hemoglobin, aspartate transaminase, and alanine transaminase levels.…”