Feed quality can be influenced by both the microbial population levels and types of organisms that become associated with the feed matrices during processing and storage. Evaluation of microbial quality of animal feeds is difficult, because feeds generally contain a diverse bacterial population that can fluctuate widely depending on a variety of factors. Microbial diversity may be investigated in animal feed using the polymerase chain reaction (PCR) and 16S rDNA primers. In this study a bacterial isolation step involving centrifugation was combined with several DNA extraction techniques, and PCR amplicons were visualised on electrophoresis gels. Seven different animal feeds and two commonly used feed ingredients, either fresh or stored for approximately 14 months at À20°C, were chosen (18 feed sources in total) to represent a variety of different matrices, concentrations of macronutrients such as protein and fat, and particle sizes. DNA extraction involving polyethylene glycol 8000 appeared to be the most reliable protocol for the extraction of community DNA for PCR analysis of feeds. The majority of the feeds (14 out of 18 animal feeds and feed ingredients) examined in this study yielded at least one PCR-positive replicate (1.