Microbiological quality (pathogen E. coli bacteria) in the coastal environment of Dumai City, Riau Province

Abstract

Escherichia coli is a water indicator microorganism. E. coli is found in almost water bodies such as lakes, rivers and seas that come from the feces of human and warm-blooded animals and waters contaminated with organic wastes. Microbiological quality is based on the presence of certain groups of microbes such as microbial pathogens (stomach disease), pollutants (especially E. coli), toxin producers etc. This research was conducted in October 2018. The purpose of this study was to calculate the density of E. coli bacteria in the coastal city of Dumai. This study used the survey method, with 5 stations with 3 sampling points at each station. Calculation of the number of Escherichia coli colonies used the MPN (Most Probable Number) method. The density of E. coli bacteria ranged from 2.37 × 104 to 1.1 × 105 CFU / 100 ml. The highest number of Escherichia coli bacterial colonies was at stations 2 and 3, the lowest was at station 5. The results of the E. coli bacteria resistance test for seven isolates of E. coli and the results of Escherichia coli bacteria resistance to antibiotics showed that there were seven isolates of Chloramphenicol, which included resistance with inhibitory zones ranging from 2.17 to 10.70 mm, 9 bacterial isolates including intermediates with inhibitory zones ranging from 11.90 - 19.33 mm, and one isolate including sensitive with a zone of inhibition of 22.77 mm. In the Penicillin and Isoniazid antibiotics all resistant bacterial isolates with inhibition zones ranged from 0.80 to 4.70 mm and 1.50 - 4.67 mm respectively. The coastal waters of the city of Dumai have passed the threshold for cultivation.

Biodiversity of Escherichia coli bacterial resistance to multidrug isolated on the Dumai coast of Indonesia

“…However, the density of E. coli at stations 2 and 3 (1.1 x 10 3 CFU/100 mL), known as an area prone to anthropogenic pollution (Feliatra et al 2020;Feliatra et al 2021), showed a higher coliform count because of the location near the harbor and densely settlements. Conversely, station 4 (7.45 x 10 2 CFU/100 mL) features a mangrove habitat, while station 1 (4.23 x 10 2 CFU/100 mL) is an industrial area (Lukistyowati et al 2019). Indonesia's Ministry of Environment is in line with the international regulations issued by WHO, which stipulated a maximum limit of E. coliform (≤200 CFU/100 mL) for activities in the field of aquaculture (Santé et al 2004).…”