Abstract. Feliatra F, Mardalisa M, Efendi I, Adelina A, Feliatra VA. 2021. Biodiversity of Escherichia coli bacterial resistance to multidrug isolated on the Dumai coast of Indonesia. Biodiversitas 23: 10-16. Anthropogenic pollution around the Dumai coast of Indonesia disturbs marine microorganisms, including commensal bacteria such as fecal Escherichia coli. Escherichia coli have often been used as a sensitive indicator for the spread of Antibiotic Resistance Genes (ARGs) among pathogens. This study aimed to analyze the development of E. coli resistance, originating from the Dumai sea waters using six antibiotics against seven E. coli isolates (E1, E4, E6, E8, E11, E13, and E15) at the five stations. Furthermore, a sensitivity test was performed on Mueller Hinton Agar (MHA) solid media, based on the Kirby-Bauer disk diffusion method. The results showed 100% E. coli isolates resistant to narrow-spectrum antibiotics (Penicillin, Isoniazid, Streptomycin, and Erythromycin) at the five stations. Meanwhile, high resistance of E. coli against the broad-spectrum antibiotics was observed in station 4 (mangrove habitat) existed in Chloramphenicol, whilst no resistance was found in Ciprofloxacin. Escherichia coli isolates showed Multidrug Resistant (MDR) index value higher than 0.2 which suggests there is a high-risk of antibiotics pollution in Dumai seawater. Bioinformatics analysis of 16S rRNA gene sequences represents the identity of E. coli isolates at the species level (97.84% - 99.74%). This study revealed the presence of antibiotic pollution in Dumai seawater which may impact public health. Hence, the local community must be discipline about the use of antibiotics freely.

No abstract

One of the interesting and environmentally friendly microbiology strategies and approaches to control the impact of microplastics is to approach bioremediation technology by harnessing the potential of microbes or indigenous bacteria (local bacteria).  Dumai sea waters currently show a high enough of microplastic pollution which allows the potential of indigenous bacteria to adapt to a plastic environment. The purpose of this study is to isolate and identify indigenous bacteria to degrade plastics from the sea waters of Dumai and to know whether or not there is a difference in the number of bacteria found between stations in this study. This research was conducted in October-December 2020 with experimental methods at the Marine Microbiology Laboratory Faculty of Fisheries and Marine Science Universitas Riau. Based on the results of the study, 12 bacterial isolates were isolated from the research stations.  Isolates of these bacteria have diamaters ranging from 0.2-1.1 cm. Microplastic degradation test results by bacteria found that ISL 10 is an isolate that shows the highest PET degradation activity, which is 17.27% and the diameter of biofilm formation 0.8 cm. Based on biochemical and morphological tests, similar results were obtained that ISL 10 bacteria are a bacterium of the genus Bacillus. The most bacterial colonies were seen in statiun IV (TPI) with an average number of bacteria of 214.9 x 104 CFU/ml.

Desa Teluk Rhu merupakan desa yang memiliki potensi dalam hal perikanan dan wisata bahari. Sebagian masyarakat di desa ini berprofesi sebagai nelayan, dan selebihnya berprofesi sebagai pembudidaya ikan. Minimnya pengetahuan dan kompetensi yang dimiliki nelayan di Desa Teluk Rhu menyebabkan tingkat kesejahteraan nelayan relatif rendah. Kegiatan pengabdian kepada masyarakat dilakukan sebagai upaya peningkatan pendapatan nelayan di Desa Teluk Rhu Kabupaten Bengkalis melalui pemanfaatan teknologi diversifikasi pangan yang berbasis pada hasil perikanan Ada empat metode yang digunakan dalam kegiatan ini, yaitu observasi, penyampaian materi, praktik pembuatan produk, dan evaluasi program. Dua puluh nelayan menjadi peserta dan mengikuti seluruh kegiatan dengan sangat antusias dan aktif. Ada dua materi yang disampaikan guna meningkatkan pengetahuan nelayan, antara lain literasi digital dan produk inovasi pangan. Pada tahap akhir, seluruh peserta dilatih untuk membuat beberapa produk diversifikasi pangan berbasis hasil perikanan seperti bakso ikan, nugget, dan stik ikan. Data kuesioner menunjukkan bahwa program pengabdian masyarakat yang telah dilaksanakan memberi banyak bagi sebagian besar nelayan di Desa Teluk Rhu. Dari kegiatan ini diharapkan, pengetahuan baru yang didapat para nelayan dapat membuat mereka lebih semangat dan termotivasi untuk memulai usaha baru guna meningkatkan pendapatan keluarga.