Microbiological Studies on the Performance of a Laminar Airflow Biological Cabinet

McDade, Joseph J.; Sabel, Fred L.; Akers, Ronald L.; Walker, Robert

doi:10.1128/aem.16.7.1086-1092.1968

Cited by 21 publications

(9 citation statements)

References 4 publications

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“…Excessive vertical airflow resulted in air possibly laden with microorganisms escaping the confines of the cabinet. These data were corroborated by McDade et al (128) with microbiological testing. These investigators also confirmed that the modifications were effective because the cabinet provided personnel, product, and environmental protection.…”

Section: Class II Safety Cabinetssupporting

confidence: 71%

“…The ideal location for a safety cabinet is a remote area such as the back wall of the laboratory well away from personnel traffic, doors, and air currents produced by heating and air conditioning vents, with due consideration for safe evacuation in the event of a fire or explosion. Rapid movements of the body as well as rapid opening and closing of laboratory doors create air currents that disrupt critical airflows at the access opening of the safety cabinet and allow test microorganisms to escape from the safety cabinet into the laboratory (38,112,128). Rake 168demonstrated that cross drafts cause an outflow of organisms from the cabinet.…”

Section: Installation Of Safety Cabinetsmentioning

confidence: 99%

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Biological safety cabinetry.

Kruse¹,

Puckett²,

Richardson³

1991

Clinical Microbiology Reviews

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tively, not divine origin, induced disease. In 1546, Fracastorius described the agent of communicable disease as a living contagium vivum that spread by direct contact, by intermediary fomites, or through the air and postulated that these living seeds, passed from one infected animal, produced the same disease in an animal that received them. In 1676, Leeuwenhoek discovered and described bacteria, but bacteriology as a science dates from the middle of the 19th century, a result of the scientific studies of Louis Pasteur. Microbiologists are cognizant of his study disproving the theory of spontaneous generation and his work with cultures and the propagation of bacteria; however, because all of the media were fluid, these methods were not applicable for isolating a single microorganism from mixed cultures. Robert Koch's studies with nutrient gelatin and, later, solid 207

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Section: Class II Safety Cabinetssupporting

confidence: 71%

Section: Installation Of Safety Cabinetsmentioning

confidence: 99%

Biological safety cabinetry.

Kruse¹,

Puckett²,

Richardson³

1991

Clinical Microbiology Reviews

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“…Lami nar airflow, first applied in industry, is widely used in medicine and micro biology (131). Biological safety cabinets with vertical laminar flow of HEPA-fi ltered air protect both the product and the worker (24,39,99) and are recommended for work where absolute containment is not necessary (9,135,194). In situations where absolute containment is required, closed cabinets maintaining negative air pressure and providing for work to be performed through ports with attached rubber gloves are indicated (9,22).…”

Section: Protective Barriersmentioning

confidence: 99%

Laboratory-Associated Infections: Incidence, Fatalities, Causes, and Prevention

Pike¹

1979

Annu. Rev. Microbiol.

205

113

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“…Some currently marketed cabinets have been tested with various biological challenge aerosols under static conditions (1,5,10,12,13,15,20), but few have been tested quantitatively under dynamic or adverse conditions. Because the function of the cabinet depends upon the critical control of airflows, it would be expected that anything that blocked or disrupted these airflows would have a direct effect upon performance.…”

mentioning

confidence: 99%

“…Other factors affecting safety cabinet perfornance are the airflow pattern and the degree of air turbulence within the room. McDade et al (13) reported poorer results from the product protection testing of their commercial cabinet when the room airflow system was turned on. They attributed this to the "induction-type" diffuser for the room air supply, which created a turbulent airflow of velocities greater than any used by the cabinet.…”

mentioning

confidence: 99%

Influence of crossdrafts on the performance of a biological safety cabinet

Rake¹

1978

Appl Environ Microbiol

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A biological safety cabinet was tested to determine the effect of crossdrafts (such as those created by normal laboratory activity or ventilation) upon the ability of the cabinet to protect both experiments and investigators. A simple crossdraft, controllable from 50 to 200 feet per min (fpm; 15.24 to 60.96 m/min), was created across the face of the unit. Modifications of standardized procedures involving controlled bacterial aerosol challenges provided stringent test conditions. Results indicated that, as the crossflow velocities exceeded 100 fpm, the ability of the cabinet to protect either experiments or investigators decreased logarithmically with increasing crossdraft speed. Because 100 fpm is an airspeed easily achieved by some air conditioning and heating vents (open windows and doorways may create velocities far in excess of 200 fpm), the proper placement of a biological safety cabinet within the laboratory--away from such disruptive air currents--is essential to satisfactory cabinet performance.

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Microbiological Studies on the Performance of a Laminar Airflow Biological Cabinet

Cited by 21 publications

References 4 publications

Biological safety cabinetry.

Biological safety cabinetry.

Laboratory-Associated Infections: Incidence, Fatalities, Causes, and Prevention

Influence of crossdrafts on the performance of a biological safety cabinet

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