Microbiological, ultrastructural and spectroscopic analyses of the anti-tooth-plaque properties of fluoride compounds in vitro

Tinanoff, Norman; Camosci, David

doi:10.1016/0003-9969(80)90065-5

Cited by 22 publications

(9 citation statements)

References 25 publications

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“…The results in these series of experiments confirm our earlier findings that SnF2 has anti-bacterial properties against S. mutans, and this effect appears related to the up- take and retention of tin by this organism (Tinanoff and Camosci, 1980). However, the present studies show that compounds similar to SnF2 in pH, ions, or valence have little or no effect on S. mutans growth or colonization.…”

Section: Discussionsupporting

confidence: 93%

“…Metal salts, especially those with high atomic weights, are generally regarded as having anti-bacterial properties (Isenberg, 1971). SnCl2, however, has been previously noted to have little anti-microbial activity (Tinanoff and Camosci, 1980). This compound is unstable in aqueous solutions (Myers, 1968), which probably reduces the tin available to the bacteria.…”

Section: Discussionmentioning

confidence: 98%

“…(2) the oxidization of thiol groups of bacterial enzymes by tin ; (3) the alteration of bacterial metabolism due to uptake of tin by bacteria (Attramadal and Svatun, 1980;Tinanoff and Camosci, 1980); or (4) the naturally low pH of SnF2 causing HF formation, which is reportedly more anti-bacterial than F (Whitford etal., 1977).…”

Section: Introductionmentioning

confidence: 98%

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Anti-bacterial Determinants of Stannous Fluoride

Camosci

Tinanoff

1984

J Dent Res

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The growth of bacteria on stainless steel wires was used as a model to investigate which properties of SnF2 produced anti-bacterial effects against S. mutans. Wire-adherent bacteria were exposed for one min, twice a day, to various fluoride or control compounds having similar ions, pH, valence, or atomic weights. After two days, the thickness of adherent bacteria was scored visually, and the decrease in pH of the growth medium was determined. Bacteria from each wire were then dried, weighed, and analyzed for metal content. Electron microscopy and electron microprobe were used to identify the location of heavy metal deposits in the bacteria. Only SnF2 dramatically altered S. mutans growth and metabolism, and this anti-bacterial effect was associated with a large uptake of tin into the bacterial cells. The fluoride salts of sodium, lead, zinc, and copper had little influence on S. mutans growth in this test system. The pH of the various fluoride salts or controls generally had no effect on the activity of the test compounds, except for the noted inactivation of SnF2 at elevated pH's. Since SnF4 did not alter the growth or metabolism of S. mutans, a unique property of SnF2--possibly the reactivity in an aqueous environment--may be responsible for its anti-bacterial properties.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 98%

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Anti-bacterial Determinants of Stannous Fluoride

Camosci

Tinanoff

1984

J Dent Res

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“…This product will, however, probably not form after short-term applications of low concentrations of stannous fluoride in vivo (18,19), and is thus probably not present on our samples. Stannous fluoride has a marked inhibiting effect both on formation of dental plaque and on the metabolism of oral bacteria as demonstrated in vivo (20)(21)(22)(23)(24)(25)(26)(27) and in vitro (28,29). This aspect of the effect of stannous fluoride probably gives an additional benefit in caries inhibition.…”

Section: Discussionmentioning

confidence: 94%

Scanning electron microscope and electron microprobe study of reactions of stannous fluoride and stannous chloride with dental enamel

Ellingsen

1986

European J Oral Sciences

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Ellingsen JE: Scanning electron microseope and electron microprobe study of reactions of stannous fluoride and stannous chloride with denial enamel. Scand J Dent Res 1986; 94: 299-305.Abstract -Stannous fluoride but not stannous chloride has shown a caries inhibiting effect in animal studies. The eJOfecJ of aqtieous solutions of stannous fluoride and stannous chloride on dental enamel surfaces was investigated by scanning electron microscope and analytical electron microprobe. Some large globules and a condnuous layer of small globules were observed after stannous fluoride treatment. The large globules were soluble in both water and alkali, whereas tbe small ones were only soluble in alkali. Both fluoride and tin were found to be present on the enamel surfaces by the electron microprobe after stannous fltioride treatment. The fluoride could not be detected after immersion in distilled water whereas alkali treatment removed the tin. The large fluoride-containing globules may consist of calcium fluoride as this substance is known to be slightly soluble in water and soluble in alkali. Stannous chloride treatment gave a layer of small tin-containing globules with properties similar to the layer formed on enamel treated with stannous fluoride. It is therefore suggested that the dn-eontaining layer is not associated with caries protection whereas calcium fluoride-like deposidon on the enamel probably is a factor in the caries inhibition caused by stannous fltioride.Stannous fluoride has been successfully used one possible aspect of die caries inhibition,, in caries prevention for a long time (1-5). and evidence for the presence of SnaFsPOj The mechanism of the caries inhibiting effect crystals on the enamel surface has been preof this compound is not completely undersented in several publications (6-9). It is stood. Reaction products between stannous knovifn that stannous fluoride, but not stanfluoride and enamel have been discussed as nous chloride, inhibits caries in rats (10, 11).

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“…Besides the poor potential of other antiplaque agents on rodent sulcal lesions [McDonald and Stookey, 1978], the concentration of the SnF2 could have possibly been too low to have an antimicrobial effect. Antiplaque properties of SnF2 at mouthrinse concen trations (100-1,000 ppm F") are known to improve as the concentration of SnF2 in creases [Tinanoff and Camosci, 1980;Svatun et al, 1977]. Furthermore, very dilute solutions of SnF2, as in the present experi ment, may quickly lose soluble stannous ions [Heferren, 1963].…”

mentioning

confidence: 85%

Effect of NaF and SnF₂in Drinking Water on Hamster Dental Caries (Short Communication)

Tinanoff¹,

Camosci²

1984

Caries Res

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Microbiological, ultrastructural and spectroscopic analyses of the anti-tooth-plaque properties of fluoride compounds in vitro

Cited by 22 publications

References 25 publications

Anti-bacterial Determinants of Stannous Fluoride

Anti-bacterial Determinants of Stannous Fluoride

Scanning electron microscope and electron microprobe study of reactions of stannous fluoride and stannous chloride with dental enamel

Effect of NaF and SnF₂in Drinking Water on Hamster Dental Caries (Short Communication)

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Microbiological, ultrastructural and spectroscopic analyses of the anti-tooth-plaque properties of fluoride compounds in vitro

Cited by 22 publications

References 25 publications

Anti-bacterial Determinants of Stannous Fluoride

Anti-bacterial Determinants of Stannous Fluoride

Scanning electron microscope and electron microprobe study of reactions of stannous fluoride and stannous chloride with dental enamel

Effect of NaF and SnF2in Drinking Water on Hamster Dental Caries (Short Communication)

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Effect of NaF and SnF₂in Drinking Water on Hamster Dental Caries (Short Communication)