Microbiology and biofilm of corneal sutures

Adler, Erik; Miller, Darlene; Rock, Oded; Spierer, Oriel; Forster, Richard K.

doi:10.1136/bjophthalmol-2018-312133

Cited by 7 publications

(6 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In suture-related PKIK cases, infected corneal sutures should also be sent for microbiological culture as they may provide additional information. Adler et al ( 107 ) evaluated the presence of microbial growth and biofilm formation amongst corneal sutures removed following astigmatic correction (quiescent), loosening or breakage (exposed), or infection. Biofilms are composed of extracellular matrix secreted by microorganisms and are usually resistant to conventional antimicrobial treatment ( 108 , 109 ).…”

Section: Diagnosismentioning

confidence: 99%

“…Biofilms are composed of extracellular matrix secreted by microorganisms and are usually resistant to conventional antimicrobial treatment ( 108 , 109 ). They have been shown to form on biotic and abiotic surfaces, including sutures ( 107 , 110 ). In their study, the infection group demonstrated a culture yield of 60% based on corneal sutures, underlining corneal suture material (when infected) as a useful source for obtaining microbiological diagnosis.…”

Section: Diagnosismentioning

confidence: 99%

See 1 more Smart Citation

Post-keratoplasty Infectious Keratitis: Epidemiology, Risk Factors, Management, and Outcomes

et al. 2021

View full text Add to dashboard Cite

Post-keratoplasty infectious keratitis (PKIK) represents a unique clinical entity that often poses significant diagnostic and therapeutic challenges. It carries a high risk of serious complications such as graft rejection and failure, and less commonly endophthalmitis. Topical corticosteroids are often required to reduce the risk of graft rejection but their use in PKIK may act as a double-edged sword, particularly in fungal infection. The increased uptake in lamellar keratoplasty in the recent years has also led to complications such as graft-host interface infectious keratitis (IIK), which is particularly difficult to manage. The reported incidence of PKIK differs considerably across different countries, with a higher incidence observed in developing countries (9.2–11.9%) than developed countries (0.02–7.9%). Common risk factors for PKIK include the use of topical corticosteroids, suture-related problems, ocular surface diseases and previous corneal infection. PKIK after penetrating keratoplasty or (deep) anterior lamellar keratoplasty is most commonly caused by ocular surface commensals, particularly Gramme-positive bacteria, whereas PKIK after endothelial keratoplasty is usually caused by Candida spp. Empirical broad-spectrum antimicrobial treatment is the mainstay of treatment for both PKIK, though surgical interventions are required in medically refractory cases (during the acute phase) and those affected by visually significant scarring (during the late phase). In this paper, we aim to provide a comprehensive overview on PKIK, encompassing the epidemiology, risk factors, causes, management and outcomes, and to propose a treatment algorithm for systematically managing this challenging condition.

show abstract

Section: Diagnosismentioning

confidence: 99%

Section: Diagnosismentioning

confidence: 99%

Post-keratoplasty Infectious Keratitis: Epidemiology, Risk Factors, Management, and Outcomes

et al. 2021

View full text Add to dashboard Cite

show abstract

“…The AM graft could be secured with sutures or fibrin glue. However, sutures may cause stitch reaction and elevate the risk of stitch abscess, granulation formation and infection [17]. Sutureless AM graft with fibrin glue has the potential to drop due to gravity or during blinking.…”

Section: Discussionmentioning

confidence: 99%

Self-Retained, Sutureless Amniotic Membrane Transplantation for the Management of Ocular Surface Diseases

Chiu,

Tsai

2023

JCM

View full text Add to dashboard Cite

Amniotic membrane (AM) has anti-inflammation, anti-fibrotic, and regenerative effects. Sutureless cryopreserved AM transplantation, ProKera® (Bio-Tissue, Inc., Miami, FL, USA), is easily applied by ophthalmologists in the treatment of ocular surface diseases. This retrospective study included patients with ocular surface diseases who received ProKera® between January 2022 and May 2023. Six patients (9 eyes) with a mean age of 56.8 ± 20.8 years old (range 25–74) and a mean follow-up period of 7.8 ± 4.1 months (range 1–12) were included, including 2 of recurrent conjunctival tumors with limbal and corneal involvement (cases 1–2), 1 of pterygium with marked astigmatism (case 3) and 3 of Stevens–Johnson syndrome (SJS, cases 4–6). ProKera® was inserted after the lesion excision and deep keratectomy in cases 1–3, and no recurrence or corneal complication was noted. Cases 4–5 were discharged from the intensive care unit and presented with severe chronic SJS. Most ocular manifestations improved significantly after symblepharon release and ProKera® insertion, except for corneal conjunctivalization in 1 eye (case 5). Case 6 involved early ProKera® use at the bedside during acute SJS, resulting in complete resolution. We concluded that the adjunctive application of ProKera® can be effective for ocular surface reconstruction and provides options to intervene earlier for outpatients or patients unstable for invasive surgical intervention.

show abstract

“…Current AMT techniques involve suturing the graft or patch of AM over the ocular surface. Nevertheless, the corneal sutures can not only cause postoperative pain and discomfort to patients [25] but also lead to complications such as additional trauma, suture abscesses [26,27], infections [28,29], granuloma formation [30], and tissue necrosis [31]. Furthermore, the inflammatory effect of corneal suture [32] can lead to an unsuccessful repairing attempt, especially if the corneal damage is due to an inflammatory disease.…”

Section: Discussionmentioning

confidence: 99%

Sutureless Amniotic Membrane Transplantation in Inflammatory Corneal Perforations

et al. 2022

View full text Add to dashboard Cite

Introduction: The aim of this study was to evaluate the efficacy of sutureless amniotic membrane transplantation (SAMT) in patients with corneal perforation secondary to ocular surface inflammatory diseases. Methods: Twelve eyes of eleven patients with corneal perforation associated with Sjögren’s syndrome and ocular cicatricial pemphigoid were included. Surgical procedure consisted in the application of two layers of amniotic membrane covering the corneal perforation and a therapeutic contact lens placed above the two layers, followed by sutureless tarsorrhaphy with Steri-strip applied for 15 days to immobilize the eyelids. All patients underwent slit-lamp examination, fluorescein corneal staining, and anterior segment optical coherence tomography (AS-OCT), preoperatively and post-surgery, weekly for one month, and then every two weeks for three months. Results: All 12 eyes had complete resolution of corneal perforation. Pachymetry measurements improved in all eyes, and complete healing of the corneal ulcer was obtained following treatment and maintained up to 3 months follow-up in all patients. Conclusions: SAMT was proven to be a safe and effective option for the management of inflammatory corneal perforation. The procedure is non-traumatic and easy to perform since the surgical technique is sutureless and does not require topical therapy because it takes advantage of the intrinsic properties of the membrane itself.

show abstract

Microbiology and biofilm of corneal sutures

Cited by 7 publications

References 14 publications

Post-keratoplasty Infectious Keratitis: Epidemiology, Risk Factors, Management, and Outcomes

Post-keratoplasty Infectious Keratitis: Epidemiology, Risk Factors, Management, and Outcomes

Self-Retained, Sutureless Amniotic Membrane Transplantation for the Management of Ocular Surface Diseases

Sutureless Amniotic Membrane Transplantation in Inflammatory Corneal Perforations

Contact Info

Product

Resources

About