Microbiology of the pericoronal pouch in mandibular third molar pericoronitis

Abstract

The microorganisms associated with mandibular third molar pericoronitis were investigated using direct microscopy and anaerobic culture method. The pericoronal pouch was sampled with paper points in A) 8 patients without mandibular third molar pericoronitis and B) 6 patients with mandibular third molar pericoronitis. Under the microscope, the microflora was found to be a complex mixture comprising gram-positive and gram-negative cocci, rods and filaments (including fusiform and curved rods), motile rods and spirochetes. Significantly higher proportions of motile, gram-negative rods were found in group B than in group A. The predominant cultivable microflora of 9 samples: A (4) and B (5) comprised several species of facultative and obligate anaerobic bacteria, namely Peptostreptococcus, Streptococcus, Actinomyces, Eubacterium, Propionibacterium, Veillonella, Porphyromonas, Prevotella, Bacteriodes, Fusobacterium, Campylobacter, Staphylococcus, Stomatococcus, Lactobacillus, Neisseria, Capnocytophaga, Haemophilus, Selenomonas and Centipeda species. The microflora in pericoronitis appeared similar to that of diseased periodontal pockets.

Evaluationof the Mandibular Third Molar Pericoronitis Flora and ItsSusceptibility to Different Antibiotics PrescribedinFrance

“…The microbial flora that develops in the distally located pseudopocket is the major cause. This flora is predominantly anaerobic (3,14,17,19,20,23,26). Therapeutic management usually involves a local surgical procedure and the prescription of antibiotics, often of the ␤-lactam family (7).…”

Flore associée aux péricoronarites des troisièmes molaires mandibulaires. Composition et sensibilité aux antibiotiques

“…Data adapted from those previously published [3] [34] . Ces résultats corroborent ceux parus dans la littérature à l'aide de sondes ADN [6] , de milieux non sélectifs [7,8] ou sélectifs [9][10][11] .…”

“…Le taux de détection de ces bactéries varie selon les études [6][7][8][9][10]12] . Des variations dans les populations et les méthodes utilisées peuvent expliquer ces différences.…”

Periodontal bacteria in different sampling sites of pericoronitis patients

“…Porphyromonas gingivalis (P.g.) has been detected in 100% of the samples of Leung et al [3], but only in 9.1% of the samples of Rajasuo et al [4] and in 20% of healthy third-molar pericoronal pockets by Mombelli et al [5]. Aggregatibacter actinomycetemcomitans (A.a.) has been detected in 9.1% [4], in 17% -40% [5] and in 10.8% [6] of the samples, respectively.…”

“…[1,2]. The microbiota of pericoronitis resembles that of gingivitis and periodontitis [3]. However, principal periodontal pathogens have been rarely isolated from pericoronitis samples by culturing.…”