Microbiome Diversity and Cellulose Decomposition Processes by Microorganisms on the Ancient Wooden Seawall of Qiantang River of Hangzhou, China

Wang, Bowen; Qi, Miaoyi; Ma, Yushu; Zhang, Bingjian; Hu, Yulan

doi:10.1007/s00248-023-02221-x

Cited by 5 publications

(3 citation statements)

References 41 publications

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“…Consistent results, with high levels of cellulase production by P. solitum and moderate levels by P. flavigenum and P. griseofulvum, were reported by Yoon et al [42]. In a study by Zyani et al [41], high levels of cellulase production were documented for three Penicillium species (P. chrysogenum, P. commune, and P. granulatum), while weak production was observed for P. crustosum, and P. expansum. These species were isolated from 450-year-old wooden constructions at the Medina of Fez (Morocco) and screened on CMC agar plates.…”

Section: Discussionsupporting

confidence: 73%

“…), are primary agents responsible for breaking down the main components of wood into simple molecules, used as nutrient sources ([bio]chemical assimilatory biodeterioration). Many soft-rot fungi, such as representatives of Trichoderma, Fusarium, and Penicillium genera, possess a complete set of enzymes, capable of the efficient degradation of cellulose [41]. Isolates of all three genera were screened for cellulase production; however, only four Penicillium species (P. flavigenum, P. griseofulvum, P. solitum, and P. speluncae) demonstrated various levels of cellulolytic production, while cultures of T. zeloharzianum and F. langsethiae lacked observable transparent zones around colonies grown on CMC agar plates.…”

Section: Discussionmentioning

confidence: 99%

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Characterization of Culturable Mycobiome of Newly Excavated Ancient Wooden Vessels from the Archeological Site of Viminacium, Serbia

Djokić,

Knežević,

Savković

et al. 2024

JoF

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Two ancient wooden vessels, specifically a monoxyle (1st century BCE to 1st century CE) and shipwreck (15th to 17th century CE), were excavated in a well-preserved state east of the confluence of the old Mlava and the Danube rivers (Serbia). The vessels were found in the ground that used to be river sediment and were temporarily stored within the semi-underground exhibition space of Mammoth Park. As part of the pre-conservation investigations, the primary aim of the research presented was to characterize the culturable mycobiomes of two excavated wooden artifacts so that appropriate conservation procedures for alleviating post-excavation fungal infestation could be formulated. Utilizing culture-based methods, a total of 32 fungi from 15 genera were identified, mainly Ascomycota and to a lesser extent Mucoromycota sensu stricto. Soft-rot Ascomycota of genus Penicillium, followed by Aspergillus and Cephalotrichum species, were the most diverse of the isolated fungi. Out of a total of 38 isolates, screened on 7 biodegradation plate assays, 32 (84.21%) demonstrated at least one degradative property. Penicillium solitum had the highest deterioration potential, with a positive reaction in 5 separate plate assays. The obtained results further broaden the limited knowledge on the peculiarities of post-excavation soft-rot decay of archaeological wood and indicate the biochemical mechanisms at the root of post-excavation fungal deterioration.

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Section: Discussionsupporting

confidence: 73%

Section: Discussionmentioning

confidence: 99%

Characterization of Culturable Mycobiome of Newly Excavated Ancient Wooden Vessels from the Archeological Site of Viminacium, Serbia

Djokić,

Knežević,

Savković

et al. 2024

JoF

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“…Ortiz et al collected wood samples from eight historic churches in Chiloé , identifying fungi species such as Coniophora, Laetiporus, and Postia (causing brown rot), as well as Trichoderma, Alternaria, and Verticillium (associated with wood decay) at various stages of decay (Ortiz et al, 2014). Wang et al utilized high-throughput sequencing to identify Bacteroidetes, Proteobacteria, Firmicutes, and Actinobacteria as the primary sources of bacterial cellulose-decomposing enzymes in an ancient wooden seawall in the Qiantang River of Hangzhou and they also isolated 11 cellulose-decomposing strains (Wang et al, 2023). Ancestral halls, traditional Chinese wooden architecture prevalent in southern China, date back to the Ming (AD1368 to AD1644) and Qing (AD1636 to AD1912) Dynasties.…”

Section: Introductionmentioning

confidence: 99%

Insights into the Microbial Weathering of Chinese Wooden Ancestral Halls in Guangdong Province

Liu

Wen

et al. 2023

Preprint

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Wooden buildings are facing biodeterioration because of natural weathering. Ancestral halls are traditional Chinese wooden architecture with high artistic value. The research analyzed nine ancestral halls with different visitor flow rates in Guangdong Province under subtropical monsoon climates. With amplicon sequencing and conventional culturing methods, a common core harmful microorganism group which are proven to be capable of degrading cellulose and lignin was found, and some of them originated from human activities, including Bacillus, Pseudomonas, Paenibacillus, Acinetobacter, Toxicocladosporium, Cladosporium, Aspergillus, and Epicoccum, allowing us to apply unified antimicrobial methods to different ancestral halls in Guangdong Province. Additionally, the microbial community is similar between damaged and undamaged points, predicting the potential risk of taking paint coating as the only method for antimicrobial preservation. Hence, we evaluated the effect of four representative biocides and determined the feasibility of low-concentration Isothiazolinone. This research adds significant reference for the protection of wooden buildings.

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Red sandstone surface microbiota: functional convergence despite taxonomic divergence under dispersal limitation and hetero selection

Wang,

Zhu,

Wang

et al. 2024

Preprint

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Understanding the role of microbiota on stone surfaces is essential for developing effective grottoes conservation strategies. However, ecological feature of microbial communities on stone surfaces remains underexplored. In this study, we explored diversity, ecological feature, and functional profiles of microbial communities on the red sandstone surface of the Leshan Giant Buddha from microbial ecology perspective. The results show that Proteobacteria, Actinobacteria, and Cyanobacteria are the dominant prokaryotic phyla, and Ascomycota is the most dominant eukaryotic phylum. Interestingly, despite taxonomic divergence, the functional profiles of different types of the microbial communities remain convergent across all samples. As indicated by the ecological modelling, the interplay of dispersal limitation and hetero selection might have contributed to such species divergence, while selective pressure from the harsh environment on the stone surface promoted functional convergence. The assembly of visually distinct microbial communities is linked to a narrower ecological niche, higher proportion of habitat specialists, elevated complexity, and increased resilience of the prokaryotic network to disturbances. Microbial-mediated ammonium assimilation and nitrogen mineralization might be the two prominent processes that contribute to stone biodeterioration. This study deepens our understanding of microbial community assembly mechanism on stone cultural heritage surfaces and functional potentials, which provides microbial ecological insights for the conservation of these cultural treasures.

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Microbiome Diversity and Cellulose Decomposition Processes by Microorganisms on the Ancient Wooden Seawall of Qiantang River of Hangzhou, China

Cited by 5 publications

References 41 publications

Characterization of Culturable Mycobiome of Newly Excavated Ancient Wooden Vessels from the Archeological Site of Viminacium, Serbia

Characterization of Culturable Mycobiome of Newly Excavated Ancient Wooden Vessels from the Archeological Site of Viminacium, Serbia

Insights into the Microbial Weathering of Chinese Wooden Ancestral Halls in Guangdong Province

Red sandstone surface microbiota: functional convergence despite taxonomic divergence under dispersal limitation and hetero selection

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