Microbiome–metabolome analysis reveals unhealthy alterations in the composition and metabolism of ruminal microbiota with increasing dietary grain in a goat model

Mao, Shengyong; Huo, Weiguang; Zhu, Weiyun

doi:10.1111/1462-2920.12724

Cited by 208 publications

(203 citation statements)

References 70 publications

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“…This is in agreement with the study conducted by Liu et al (2014) reporting a significant increase in the proportion of Chloroflexi in the cecal microbial communities of goats fed high-grain diets. In contrast, others reported opposite responses of Chloroflexi in animals fed energy-dense diets (Hook et al, 2011; Mao et al, 2016). …”

Section: Discussionmentioning

confidence: 96%

Linking Peripartal Dynamics of Ruminal Microbiota to Dietary Changes and Production Parameters

et al. 2017

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During the peripartal period, proper acclimatization of rumen microorganisms to variations in nutritional management can facilitate the transition into lactation. This study characterized the temporal shifts in the composition and functional properties of ruminal microbiota during the periparturient period in dairy cows subjected to a typical two-tiered feeding management approach. Ruminal digesta samples from eight multiparous fistulated Holstein cows were collected on days −14, −7, 10, 20, and 28 relative to parturition. High-throughput Illumina sequencing of the V4 region of the bacterial 16S rRNA gene revealed distinct clustering patterns between pre- and postpartal ruminal microbiota. During the prepartal period, when the voluntary dry matter intake was lower, we observed strikingly lower inter-animal variations in the composition of the ruminal microbiota. Genera Ruminococcus and Butyrivibrio, which are considered major fibrolytic rumen dwellers, were overrepresented in the prepartal rumen ecosystem. In contrast, increased postpartal voluntary DMI was associated with enrichment of bacterial genera mainly consisting of proteolytic, amylolytic, and lactate-producer species (including Prevotella, Streptococcus, and Lactobacillus). These, together with the postpartal enrichment of energy metabolism pathways, suggested a degree of acclimatization of the ruminal microbiota to harvest energy from the carbohydrate-dense lactation diet. In addition, correlations between ruminal microbiota and parameters such as milk yield and milk composition underscored the metabolic contribution of this microbial community to the cow's performance and production.

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Section: Discussionmentioning

confidence: 96%

Linking Peripartal Dynamics of Ruminal Microbiota to Dietary Changes and Production Parameters

et al. 2017

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“…Nisin also increased butyrate concentration to a greater extent than monensin. Protozoa were among the major butyrate producers in the rumen (Williams and Coleman, 1997), and they were positively associated with butyrate production (Mao et al, 2015a). In the present study, protozoa were decreased by monensin but not affected by nisin.…”

Section: Discussionmentioning

confidence: 99%

Monensin and Nisin Affect Rumen Fermentation and Microbiota Differently In Vitro

Shen

Liu

et al. 2017

Front. Microbiol.

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Nisin, a bacteriocin, is a potential alternative to antibiotics to modulate rumen fermentation. However, little is known about its impacts on rumen microbes. This study evaluated the effects of nisin (1 and 5 μM) on in vitro rumen fermentation characteristics, microbiota, and select groups of rumen microbes in comparison with monensin (5 μM), one of the most commonly used ionophores in ruminants. Nisin had greater effects than monensin in inhibiting methane production and decreasing acetate/propionate ratio. Unlike monensin, nisin had no adverse effect on dry matter digestibility. Real-time PCR analysis showed that both monensin and nisin reduced the populations of total bacteria, fungi, and methanogens, while the population of protozoa was reduced only by monensin. Principal component analysis of bacterial 16S rRNA gene amplicons showed a clear separation between the microbiota shaped by monensin and by nisin. Comparative analysis also revealed a significant difference in relative abundance of some bacteria in different taxa between monensin and nisin. The different effects of monensin and nisin on microbial populations and bacterial communities are probably responsible for the discrepancy in their effects on rumen fermentation. Nisin may have advantages over monensin in modulating ruminal microbial ecology and reducing ruminant methane production without adversely affecting feed digestion, and thus it may be used as a potential alternative to monensin fed to ruminants.

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“…An increase in microbial diversity has been related to enhance ecosystem stability and resistance to pathogen invasion [28-29]. Therefore, the increased diversity in the intestinal microbiota by GSPs most likely contributed to the improved intestinal mucosa immune system [30].…”

Section: Discussionmentioning

confidence: 99%

Dietary grape seed proanthocyanidins (GSPs) improve weaned intestinal microbiota and mucosal barrier using a piglet model

et al. 2016

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Proanthocyanidins have been suggested as an effective antibiotic alternative, however their mechanisms are still unknown. The present study investigated the effects of grape seed proanthocyanidins on gut microbiota and mucosal barrier using a weaned piglet model in comparison with colistin. Piglets weaned at 28 day were randomly assigned to four groups treated with a control ration, or supplemented with 250 mg/kg proanthocyanidins, kitasamycin/colistin, or 250 mg/kg proanthocyanidins and half-dose antibiotics, respectively. On day 28, the gut chyme and tissue samples were collected to test intestinal microbiota and barrier function, respectively. Proanthocyanidins treated piglets had better growth performance and reduced diarrhea incidence ( P < 0.05), accompanied with decreased intestinal permeability and improved mucosal morphology. Gene sequencing analysis of 16S rRNA revealed that dietary proanthocyanidins improved the microbial diversity in ileal and colonic digesta, and the most abundant OTUs belong to Firmicutes and Bacteroidetes spp . Proanthocyanidins treatment decreased the abundance of Lactobacillaceae , and increased the abundance of Clostridiaceae in both ileal and colonic lumen, which suggests that proanthocyanidins treatment changed the bacterial composition and distribution. Administration of proanthocyanidins increased the concentration of propionic acid and butyric acid in the ileum and colon, which may activate the expression of GPR41. In addition, dietary proanthocyanidins improved the antioxidant indices in serum and intestinal mucosa, accompanied with increasing expression of barrier occludin. Our findings indicated that proanthocyanidins with half-dose colistin was equivalent to the antibiotic treatment and assisted weaned animals in resisting intestinal oxidative stress by increasing diversity and improving balance of gut microbes.

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Microbiome–metabolome analysis reveals unhealthy alterations in the composition and metabolism of ruminal microbiota with increasing dietary grain in a goat model

Cited by 208 publications

References 70 publications

Linking Peripartal Dynamics of Ruminal Microbiota to Dietary Changes and Production Parameters

Linking Peripartal Dynamics of Ruminal Microbiota to Dietary Changes and Production Parameters

Monensin and Nisin Affect Rumen Fermentation and Microbiota Differently In Vitro

Dietary grape seed proanthocyanidins (GSPs) improve weaned intestinal microbiota and mucosal barrier using a piglet model

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