Microbiome of dental implants and its clinical aspect

Preethanath, Reghunathan S.; Al-Nahas, Nadia W.; Huraib, Sahar M. Bin; AlBalbeesi, Hana O; Almalik, Naif Khalid; Dalati, Mohamad Hani Nouri; Divakar, Darshan Devang

doi:10.1016/j.micpath.2017.02.009

Cited by 36 publications

(31 citation statements)

References 43 publications

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“…34 Moreover, wettability/hydrophobicity and surface-free energy also influence the formation of biofilm on implants. 24,35 Moreover, as connections and prosthetic variables changed the microbiota around implants, 21,23,[36][37][38][39] as well as the peri-implant region and healthy remaining teeth, [40][41][42] our study analyzed only screw-rehabilitated prostheses in periodontally healthy individuals. This approach allows an unbiased comparison between smokers and non-smokers in relation to their peri-implant microbiome.…”

Section: Microbiome Resultsmentioning

confidence: 99%

“…Biofilm formation is directly related to the surface roughness; hence, the greater the roughness, the higher the rate of biofilm formation around the implants . Moreover, wettability/hydrophobicity and surface‐free energy also influence the formation of biofilm on implants . Moreover, as connections and prosthetic variables changed the microbiota around implants, as well as the peri‐implant region and healthy remaining teeth, our study analyzed only screw‐rehabilitated prostheses in periodontally healthy individuals.…”

Section: Discussionmentioning

confidence: 99%

“…However, there is a need to understand better the impact of smoking, to control any confounding variables. In this context, the prosthetic aspects, bone‐level height and the clinical condition of the remaining teeth are aspects that should be considered …”

Section: Introductionmentioning

confidence: 99%

“…In this context, the prosthetic aspects, bone-level height and the clinical condition of the remaining teeth are aspects that should be considered. 21,23,24 Therefore, the purpose of the present investigation was to examine the impact of smoking on the peri-implant microbiome in single, clinically healthy, screw-retained, teeth-surrounded implants, in non-periodontitis subjects.…”

mentioning

confidence: 99%

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Smoking habit modulates peri‐implant microbiome: A case‐control study

Pimentel

Fontes

Ribeiro

et al. 2018

J of Periodontal Research

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Background and Objective Smoking is a recognized risk factor for peri‐implant disease and leads to microbiological changes in mucositis and peri‐implantitis. However, there is no knowledge about the impact of smoking in healthy peri‐implant tissue. The aim of the study was to evaluate the microbiome in a peri‐implant environment in smokers with healthy peri‐implant conditions. Methods Peri‐implant biofilm was collected around single clinically healthy, screwed‐retained, teeth‐surrounded implants in 12 non‐smoker (NSMK) and 12 smoker (SMK) non‐periodontitis subjects (no bleeding and probing depth <4 mm). Bacterial DNA was isolated and 16S ribosomal RNA gene libraries were sequenced using pyrosequencing, targeting the V3‐V4 region. Datasets were processed using the Quantitative Insights into Microbial Ecology, Greengenes and the Human Oral Microbiome Database databases. Results An evident difference in the SMK peri‐implant microbiome was observed compared to the NSMK microbiome, with a large abundance of species, even with a healthy peri‐implant. The SMK core‐microbiome showed an abundance of Fusobacterium, Tannerella and Mogibacterium, while the NSMK core revealed an abundance of Actinomyces, Capnocytophaga and Streptococcus, genera that are usually related to periodontal health. The microbiome inter‐relationship was shown to be more inter‐generic in SMK then in NSMK, indicating different microbiome cohesion. Conclusion Smoking negatively affected the peri‐implant microbiome, leading to a disease‐associated state, even in clinically healthy individuals.

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Section: Microbiome Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

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Smoking habit modulates peri‐implant microbiome: A case‐control study

Pimentel

Fontes

Ribeiro

et al. 2018

J of Periodontal Research

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“…reconstructive surgery to regenerate the cancellous bone of the maxilla and mandible (5,6). In GBR, bone substitute is used to fill the defect, which is then overlaid by a barrier membrane.…”

Section: Guided Bone Regeneration (Gbr) Is An Established Technique Imentioning

confidence: 99%

Collagen Membrane for Guided Cortical Bone Regeneration: Characterization, Preclinical and Proof of Concept Clinical Studies

Allan

Ruan

Landao-Bassonga

et al. 2020

Preprint

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Background: Treatment of cortical bone defects is a clinical challenge. Guided bone regeneration (GBR), commonly used in oral in maxillofacial dental surgery, may show promise for orthopedic application in repair of cortical defects. However, a limitation in the use of GBR for cortical bone defects is the lack of an ideal scaffold that provides sufficient mechanical support to bridge the cortical bone with minimal interference in the repair process. We have developed a new collagen membrane, CelGroTM, for use in GBR. We report the material characterisation of CelGroTM, and evaluate the performance of CelGroTM in translational preclinical and clinical studies. Methods: Scanning electron microscopy (SEM), micro computed tomography (micro-CT) and transmission electron microscopy (TEM) were used to examine the structural morphology of CelGroTM. Purity and biochemical composition of CelGroTM was evaluated by Western-blot, immunohistochemistry and confocal microscopy. Physical and chemical properties of CelGroTM were examined and compared with another commercially available collagen membrane. The pre-clinical evaluation was conducted using a cortical bone defect model in the New Zealand white rabbit. Cortical bone regeneration in defects of the femoral diaphysis were evaluated at 30 days and 60 days after intervention, by micro-CT and histology. A clinical study to evaluate the performance of CelGroTM in GBR for treatment of bone augmentation surrounding dental implants was also performed. The clinical outcomes were evaluated by semi quantitative tissue condition assessments and cone-beam computed tomography (CBCT) scan. Results: CelGroTM has a bilayer structure of different fibre alignment and is composed almost exclusively of type I collagen. CelGroTM was found to be completely acellular and a clinically significant xenoantigen, α -gal, was not detected. CelGroTM displayed less deformity and better mechanical strength as compared to Bio-Gide ® . In the preclinical study, CelGroTM demonstrated enhanced bone-modelling activity and cortical bone healing. Micro-CT evaluation showed early bony bridging over the defect area 30 days post-operatively, and nearly complete restoration of mature cortical bone at the bone defect site 60 days post- operatively. Histological analysis at day 60 after surgery further confirmed that CelGroTM enables bridging of the cortical bone defect by induction of newly-formed cortical bone. It appears that CelGroTM showed better cortical alignment and reduced porosity at the defect interface compared to Bio-Gide®. Owning the fact that selection of orthopedic patients with cortical bone defects is complex, we conducted the proof of concept clinical study in a total of 16 dental implants which were placed in 10 participants receiving GBR. The results showed that there were with no complications or adverse events observed. CBCT evidenced efficiency of the CelGroTM scaffold for GBR for the dental implants, showing significantly decreased 2 distance from the implant shoulder to first bone/implant contact (DIB) and increased horizontal thickness of facial bone wall (HT). Conclusion: The findings of our study demonstrate that CelGroTM is an ideal membrane for GBR not only in oral maxillofacial reconstructive surgery but also in orthopedic applications. Details of clinical trial registration: “Single centre, open-label, pilot study of Celgro(tm) collagen membrane for guided bone regeneration around exposed implants in patients undergoing dental implant surgery”; Registration ID: ACTRN12615000027516; Date of registration: 19/01/2015; URL: https://anzctr.org.au/ACTRN12615000027516.aspx

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Antibiofilm effects of titanium surfaces modified by laser texturing and hot‐pressing sintering with silver

et al. 2021

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Peri‐implant diseases are one of the main causes of dental implant failure. New strategies for dental implants manufacturing have been developed to prevent the accumulation of bacteria and related inflammatory reactions. The main aim of this work was to develop laser‐treated titanium surfaces covered with silver that generate a electrical dipole to inhibit the oral bacteria accumulation. Two approaches were developed for that purpose. In one approach a pattern of different titanium dioxide thickness was produced on the titanium surface, using a Q‐Switched Nd:YAG laser system operating at 1064 nm. The second approach was to incorporate silver particles on a laser textured titanium surface. The incorporation of the silver was performed by laser sintering and hot‐pressing approaches. The anti‐biofilm effect of the discs were tested against biofilms involving 14 different bacterial strains growth for 24 and 72 hr. The morphological aspects of the surfaces were evaluated by optical and field emission guns scanning electronical microscopy (FEGSEM) and therefore the wettability and roughness were also assessed. Physicochemical analyses revealed that the test surfaces were hydrophilic and moderately rough. The oxidized titanium surfaces showed no signs of antibacterial effects when compared to polished discs. However, the discs with silver revealed a decrease of accumulation of Porphyromonas gingivalis and Prevotella intermedia strains. Thus, the combination of Nd:YAG laser irradiation and hot‐pressing was effective to produce silver‐based patterns on titanium surfaces to inhibit the growth of pathogenic bacterial species. The laser parameters can be optimized to achieve different patterns, roughness, and thickness of the modified titanium layer regarding the type and region of the implant.

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Microbiome of dental implants and its clinical aspect

Cited by 36 publications

References 43 publications

Smoking habit modulates peri‐implant microbiome: A case‐control study

Smoking habit modulates peri‐implant microbiome: A case‐control study

Collagen Membrane for Guided Cortical Bone Regeneration: Characterization, Preclinical and Proof of Concept Clinical Studies

Antibiofilm effects of titanium surfaces modified by laser texturing and hot‐pressing sintering with silver

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