Microbulbifer rhizosphaerae sp. nov., isolated from the rhizosphere of the halophyte Arthrocnemum macrostachyum

Camacho, M. E.; Montero‐Calasanz, Maria del Carmen; Redondo‐Gómez, Susana; Rodríguez‐Llorente, Ignacio D.; Schümann, Peter; Klenk, Hans‐Peter

doi:10.1099/ijsem.0.000955

Cited by 24 publications

(9 citation statements)

References 31 publications

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“…Members of the genus Microbulbifer are halophilic and isolated from salt-rich environments. The vast majority of them were from marine habitats, except for Microbulbifer halophilus from saline soil of Ganjiahu Natural Reserve [2] and Microbulbifer rhizosphaerae from rhizosphere of a halophytic plant [3]. During a search for alginate-degrading bacteria from marine samples, a novel strain of the genus Microbulbifer was obtained.…”

Section: Full-textmentioning

confidence: 99%

Microbulbifer harenosus sp. nov., an alginate-degrading bacterium isolated from coastal sand

Huang

et al. 2020

International Journal of Systematic and Evolutionary Microbiology

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A Gram-stain-negative, aerobic, rod-shaped bacterium with peritrichous flagella, designated strain HB161719T, was isolated from coastal sand collected from Tanmen Port in Hainan, PR China. The isolate was found to grow with 2–11 % (w/v) NaCl, at 15–45 °C and pH 6.0–10.0, with an optima of 2–3 % NaCl, 37 °C and pH 7.0, respectively. Chemotaxonomic analysis showed that Q-8 was detected as the sole respiratory quinone and that iso-C15 : 0 and summed features 3, 8 and 9 were the major cellular fatty acids. The G+C content of the genomic DNA was 58.2 mol%. Analysis of the 16S rRNA gene sequence of the strain showed an affiliation with the genus Microbulbifer , sharing 98.7, 98.4, 97.8 and 97.8 % sequence similarities to the closest relatives of Microbulbifer okinawensis ABABA23T, Microbulbifer pacificus SPO729T, Microbulbifer taiwanensis CC-LN1-12T and Microbulbifer gwangyangensis GY2T, respectively. Low DNA–DNA hybridization values showed that it formed a distinct genomic species. The combined phenotypic and molecular features supported that strain HB161719T represents a novel species of the genus Microbulbifer , for which the name Microbulbifer harenosus sp. nov. is proposed. The type strain is HB161719T (=CGMCC 1.13584T=JCM 32688T).

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Section: Full-textmentioning

confidence: 99%

Microbulbifer harenosus sp. nov., an alginate-degrading bacterium isolated from coastal sand

Huang

et al. 2020

International Journal of Systematic and Evolutionary Microbiology

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“…yueqingensis CGMCC 1.10658 T (98.0 %) and M. elongatus DSM 6810 T (97.9 %), respectively (Table S2, available in the online version of this article), which were lower than the 16S rRNA gene similarity threshold (98.65 %) for species demarcation [34–39]. In addition, sequence similarity between strains TT30 T , TT37 T and L3 T was lower than species demarcation too, except between strains TT30 T and TT37 T with the 16S rRNA gene similarity of 99.1 %.…”

Section: Resultsmentioning

confidence: 99%

Microbulbifer zhoushanensis sp. nov., Microbulbifer sediminum sp. nov. and Microbulbifer guangxiensis sp. nov., three marine bacteria isolated from a tidal flat

Kong

et al. 2023

International Journal of Systematic and Evolutionary Microbiology

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Three strains, TT30T, TT37T and L3T, were isolated from tidal flat samples. Cells were Gram-stain-negative, non-motile and rod shaped. Cells of strains TT30T and TT37T were able to grow in a medium containing 1.0–15.0 % (w/v) NaCl (optimum, 3.0 and 4.0 %, respectively), and cells of strain L3T was able to grow in a medium containing 1.0–10.0 % (w/v) NaCl (optimum, 1.0 %). Growth of the three strains was observed at pH 6.0–10.0 and at 10–40 °C. Strains TT30T, TT37T and L3T showed the highest similarity to Microbulbifer hydrolyticus DSM 11525T (97.7 %), M . yueqingensis CGMCC 1.10658T (98.0 %) and M. elongatus DSM 6810T (97.9 %), respectively. Results of phylogenetic analyses indicated that the three isolates represented two distinct lineages within the genus Microbulbifer . The DNA G+C contents of strains TT30T, TT37T and L3T were 61.3, 60.9 and 60.2%, respectively. The average nucleotide identity and in silico DNA–DNA hybridization values among strains TT30T, TT37T and L3T and the reference strains were 84.4–87.4 and 19.6–28.9 %, respectively. Differential phenotypic properties, chemotaxonomic differences, phylogenetic distinctiveness, together with the genomic data, demonstrated that strains TT30T, TT37 T and L3T represent novel species of the genus Microbulbifer , which are named Microbulbifer zhoushanensis sp. nov. (TT30T=KCTC 92167T=MCCC 1K07276T), Microbulbifer sediminum sp. nov. (TT37T=KCTC 92168T=MCCC 1K07277T) and Microbulbifer guangxiensis sp. nov. (L3T=KCTC 92165T=MCCC 1K07278T).

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“…The fourth clade (DG) was represented by the proteins of Deltaproteobacteria and non-phytopathogenic species of Gammaproteobacteria. Thus, the Svx-like proteins of Gammaproteobacteria split into phylogenetically distant groups (G and DG): the proteins of phytopathogenic and one plant endophytic Gammaproteobacteria constituted the first clade (G), while those of non-phytopathogenic/non-plant endophytic Gammaproteobacteria-Vibrio quintilis (inhabitant of sea water, [21]), Vibrio mangrovi (inhabitant of the rhizosphere of mangrove-associated wild rice, [22]), Microbulbifer rhizospaerae (inhabitant of the rhizosphere of halophilic plants, [23]), and Teredinibacter turnerae (symbiont of mollusk, [24])-located in the fourth clade (DG). Based on multi-protein phylogeny [25] and 16S rRNA sequence, Pectobacterium, Dickeya, and Samsonia are phylogenetically closer to Vibrio, Teredinibacter, and Microbulbifer than to Xanthomonas species.…”

Section: Phylogenetic Analysis Of the Svx-like Proteinsmentioning

confidence: 99%

Structure-Functional Characteristics of the Svx Protein—The Virulence Factor of the Phytopathogenic Bacterium Pectobacterium atrosepticum

Tendiuk

Konnova

Osipova

et al. 2022

IJMS

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The Svx proteins are virulence factors of phytopathogenic bacteria of the Pectobacterium genus. The specific functions of these proteins are unknown. Here we show that most of the phytopathogenic species of Pectobacterium, Dickeya, and Xanthomonas genera have genes encoding Svx proteins, as well as some plant-non-associated species of different bacterial genera. As such, the Svx-like proteins of phytopathogenic species form a distinct clade, pointing to the directed evolution of these proteins to provide effective interactions with plants. To get a better insight into the structure and functions of the Svx proteins, we analyzed the Svx of Pectobacterium atrosepticum (Pba)—an extracellular virulence factor secreted into the host plant cell wall (PCW). Using in silico analyses and by obtaining and analyzing the recombinant Pba Svx and its mutant forms, we showed that this protein was a gluzincin metallopeptidase. The 3D structure model of the Pba Svx was built and benchmarked against the experimental overall secondary structure content. Structure-based substrate specificity analysis using molecular docking revealed that the Pba Svx substrate-binding pocket might accept α-glycosylated proteins represented in the PCW by extensins—proteins that strengthen the PCW. Thus, these results elucidate the way in which the Pba Svx may contribute to the Pba virulence.

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Microbulbifer rhizosphaerae sp. nov., isolated from the rhizosphere of the halophyte Arthrocnemum macrostachyum

Cited by 24 publications

References 31 publications

Microbulbifer harenosus sp. nov., an alginate-degrading bacterium isolated from coastal sand

Microbulbifer harenosus sp. nov., an alginate-degrading bacterium isolated from coastal sand

Microbulbifer zhoushanensis sp. nov., Microbulbifer sediminum sp. nov. and Microbulbifer guangxiensis sp. nov., three marine bacteria isolated from a tidal flat

Structure-Functional Characteristics of the Svx Protein—The Virulence Factor of the Phytopathogenic Bacterium Pectobacterium atrosepticum

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