Microcalorimetric investigation of metabolism in rat hepatocytes cultured on microplates and in cell suspensions

Nässberger, Lennart; Jensen, Elmo; Monti, Mario; Florén, Claes‐Henrik

doi:10.1016/0304-4165(86)90258-8

Cited by 19 publications

(12 citation statements)

References 14 publications

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“…The decreasing power curve resembles the curves previously noted [13] for hepatocytes in suspension at high concentration 1.4 • 106 cells/ml. The heat production rate reflects the overall metabolism in the tissue examined.…”

Section: Discussionsupporting

confidence: 85%

“…This result is very close to our present value of oxygen consumption at about 70% of the total metabolism. There is also good agreement with our previous study on hepatocytes [13], which showed a reduction in heat production by 20% and 60%, respectively, when anaerobic and aerobic metabolism were inhibited by sodium fluoride and sodium azide added to the medium. The difference in the experimental design of the two studies should be taken into account.…”

Section: Discussionsupporting

confidence: 81%

“…The rates of heat production found in the present study are lower than the values previously noted for isolated hepatocytes [2,11,13], but a comparison is not possible due to differences in the experimental designs. The metabolic aerobic/anaerobic profile shows a good qualitative agreement in the different studies, including the present one.…”

Section: Discussioncontrasting

confidence: 55%

“…We have noted the same phenomenon in isolated hepatocytes [13] and also in other systems [5,10], including non-mammalian cells [14]. Other investigators have made the same observation when measuring oxygen consumption in lymphocytes and named this phenomenon the "crowding effect" [4,8].…”

Section: Discussionmentioning

confidence: 64%

“…Microcalorimetry is a sensitive technique that has been applied to the measurement of overall metabolism and heat production in biological samples. In the last decade microcalorimetric recording of liver cells has also been performed, both in suspension [2,11,13] and as microplate cultures [13]. The aim of the present study was to evaluate the possibility of performing microcalorimetric measurements of heat production in sections of liver tissue.…”

Section: Introductionmentioning

confidence: 99%

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Microcalorimetric studies on metabolism of hepatic tissue I. A methodological study of normal tissue

1990

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In the present study, the heat production of liver biopsies (5-8 mg) was measured by a microcalorimetric technique. Tissue incubated in Leibowitz L-15 medium (L-15) showed a higher metabolic rate compared to tissue incubated in a medium without substrate 2.8 microW/mg and 1.75 microW/mg, respectively. Heat production was found to be related to weight density. No difference in the metabolic rate was found after organ perfusion in comparison to nonperfused liver. Storage in medium L-15 at 4 degrees C caused a lower rate of heat production, but if the tissue was stored in an electrolyte balance solution without substrate, no difference was seen compared to fresh tissue. Recording heat production with the present calorimetric technique is relatively simple and rapid and allows measurement of small samples.

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Section: Discussionsupporting

confidence: 85%

Section: Discussionsupporting

confidence: 81%

Section: Discussioncontrasting

confidence: 55%

Section: Discussionmentioning

confidence: 64%

Section: Introductionmentioning

confidence: 99%

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Microcalorimetric studies on metabolism of hepatic tissue I. A methodological study of normal tissue

1990

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Metabolic heat production as a measure of macrophage response to particles from orthopedic implant materials

Charlebois¹,

Daniels²,

Smith³

2001

J. Biomed. Mater. Res.

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An in vitro method to gauge metabolic heat response of macrophages (MØ) to particulates is described. Whereas the majority of work cited relies on chemical analysis to assess MØ response to particles, we have used isothermal microcalorimetry (IMC) for direct continuous measurement of metabolic heat production to gauge the response. IMC is a screening method, in that it ensures that no energy-consuming phagocytic response goes undetected, and that the aggregate metabolic magnitude of the responses is determined. A four-well IMC was used in all microcalorimetric measurements. To accommodate "zero-time" monitoring of the interaction of particles and cells, a set of identical test chambers was constructed for use in the IMC. MØs were injected from outside the IMC onto particles contained in collagen or gelatin on glass coverslips at the bottom of each chamber. IMC runs were performed using MØs only, MØs and lipopolysaccharide (LPS) positive control, and MØs and clean or LPS-bound particles of either high-density polyethylene (HDPE) or cobalt-chrome alloy (CoCr). Total heat produced by the negative controls (MØs alone) was lower than for MØ exposure to LPS or particles. The trend was a higher response for LPS-bound HDPE compared with clean HDPE particles, though not significant. In conclusion, our results have shown that IMC can be used to detect the heat associated with the phagocytosis of particulate materials by MØs in vitro.

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Phospholipase A2 activity determines rate of mitochondrial respiration in hibernating animals

Brustovetskiĭ¹,

Grishina²,

Maevskii³

et al. 1989

Bull Exp Biol Med

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Microcalorimetric investigation of metabolism in rat hepatocytes cultured on microplates and in cell suspensions

Cited by 19 publications

References 14 publications

Microcalorimetric studies on metabolism of hepatic tissue I. A methodological study of normal tissue

Microcalorimetric studies on metabolism of hepatic tissue I. A methodological study of normal tissue

Metabolic heat production as a measure of macrophage response to particles from orthopedic implant materials

Phospholipase A2 activity determines rate of mitochondrial respiration in hibernating animals

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