Microcalorimetric investigation of the toxic action of Cr(VI) on the metabolism of Tetrahymena thermophila BF5 during growth

Zheng, Dan; Liu, Yi; Zhang, Yue; Chen, Xiaojuan; Shen, Yin

doi:10.1016/j.etap.2005.12.011

Cited by 45 publications

(19 citation statements)

References 20 publications

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“…The results are shown in Fig. 1, from which we can see that the metabolic process can be divided into four phases: lag phase (AB), log phase (BC), stationary phase (CD), and decline phase (DE) [26].…”

Section: Thermogenic Curvesmentioning

confidence: 98%

Bioenergetic Investigation of Action of Lithium to Tetrahymena thermophila bF5 by Microcalorimetry

Liu

Qin

et al. 2007

Biol Trace Elem Res

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Microcalorimetry was employed to investigate the action of Li(I) to aquatic ecosystem from the point view of bioenergetics. Tetrahymena thermophila BF5 was chosen as the model organism. The power-time curves of T. thermophila BF5 growth metabolism in the absence and presence of Li(I) were obtained. The corresponding thermokinetic parameters were derived. The generation time was calculated as 592.3 min, which was consistent with the biomass values. Low concentration of Li(I) (1-20 mmol l-1) stimulated the growth of T. thermophila BF5, whereas the inhibition effect was observed in high concentration (30-100 mmol l-1). The value of IC50 was 52.8 mmol l-1. In the concentration range of 30-100 mmol l-1, the growth rate constants (k) and the maximum heat out power (P max) decrease with the concentration of Li(I), whereas the heat output (Q) increases slightly compared to the control. Other than the classic mechanism of inositol-phosphate cycle, the involvement of mitochondria mechanism was discussed and suggested.

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Section: Thermogenic Curvesmentioning

confidence: 98%

Bioenergetic Investigation of Action of Lithium to Tetrahymena thermophila bF5 by Microcalorimetry

Liu

Qin

et al. 2007

Biol Trace Elem Res

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“…Under this condition, T.t.BF 5 growth was consistent with the microorganism growth curve. The growth curve could be divided into four phases [18], i.e., the lag phase/latency (A-B), the exponential phase (B-C), the stationary phase (C-D) and the decline phase (D-E). According to equation (1), lnP t and t of the exponential phase on the thermogenic curves were gained by the parallel experiments.…”

Section: Thermogenic Curves Of Ttbf 5 Growthmentioning

confidence: 99%

The toxic effect of solubilizing excipients on Tetrahymena thermophila BF5 growth investigated by microcalorimetry

et al. 2010

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The toxic effect of different solubilizing excipients on the growth and metabolism of Tetrahymena thermophila BF 5 (T.t.BF 5 ) at various concentrations was investigated by microcalorimetry. The thermogenic curves of T.t.BF 5 growth were determined at 28°C, and were evaluated by dynamic parameters. The results indicated that the values of growth rate constant (k), maximum power (P 1 , P 2 ), peak time (T 1 , T 2 ) and total quantity of heat (Q) varied for different excipients. There was a good linear relationship between k and concentrations (r>0.95, P<0.01). 5% inhibition concentration (IC 5 ) of poloxamer 188, Tween 80, PEG 600, PEG 400 and Tween 20 was 2.18, 1.07, 1.35, 0.58, and 0.045 mg/mL, respectively. After the principal component analysis (PCA), Q, k and P 1 could characterize the effect of these excipients on T.t.BF 5 growth. Comprehensive evaluation indicated that compared with the control group, poloxamer 188 had the weakest toxicity and Tween 20 had the strongest toxicity. solubilizing excipients, Tetrahymena thermophila BF 5 , microcalorimetry, IC 5 , PCA Citation: Zhang S F, Yan D, Tang H Y, et al. The toxic effect of solubilizing excipients on Tetrahymena thermophila BF 5 growth investigated by microcalorimetry.As nonionics, the structure of solubilizing excipients includes not only hydrophilic groupings, but also lipophilic groupings to form micelles or reverse micelles in the solution. Therefore, the solubilizing excipients could be used in the indissolvable liquid preparation as solubilizers or emulsifiers [1]. However, different biologic toxicities are possibly produced due to the excipient itself or the byproduct such as the ekibon, ethylene alcohol, and diethylene glycol. The security problems of the excipients, especially applied to the injection, seem to be serious. The general evaluation methods supply direct and apparent results for security evaluation of the solubilizing excipients [2], but shortcomings such as strong subjectivity and less sensitivity make the evaluation hard to be objective and quantifiable. It is necessary to develop an objective and sensitive method to evaluate biological security of the solubilizing excipients.T.t.BF 5 is a type of eukaryotic protozoa living in the fresh water which solely contains one cell. It has a representative subcellular structure which is seen among most of the eucaryons. It has similarities with cells of higher animals [3]. It contacts the outside environment through cellular membranes without cell walls. This cellular membrane could response more rapidly to the change of the outside environment than higher animals [4]. Because its growth curve corresponds with the representative growth curve of microbes, the reaction of T.t.BF 5 to the surrounding toxicant could be studied by analyzing the dynamic thermal growth curves.T.t.BF 5 has been applied in the fields of biology, pharmacy, toxicology, environment and polluted water evaluation as a perfect model [5][6][7][8][9]. However, few reports showed that it was applied to evaluate the toxicity ...

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“…Microcalorimetry, as a quick and accurate method, has been used widely in ecology and environmental sciences as in the present study [7,8] due to its high sensitivity, high accuracy, non-destructivity, and automaticity [9]. Yao et al also studied the influence of manganese on promotion of the growth metabolism and inhibition of Shanghai Tetrahymena (Tetrahymena shanghaiensis S1 99) by microcalorimetry [10]. Cd and Cu, which are toxic to the environment, were selected for the experiment of their effects on growth metabolism of T. thermophila BF5 by microcalorimetry in the paper.…”

Section: Introductionmentioning

confidence: 97%

Effects of Artemisinin Derivative on the Growth Metabolism of Tetrahymena thermophila BF5 Based on Expression of Thermokinetics

Xuesong

Zhuangping

et al. 2009

Biol Trace Elem Res

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Microcalorimetric investigation of the toxic action of Cr(VI) on the metabolism of Tetrahymena thermophila BF5 during growth

Cited by 45 publications

References 20 publications

Bioenergetic Investigation of Action of Lithium to Tetrahymena thermophila bF5 by Microcalorimetry

Bioenergetic Investigation of Action of Lithium to Tetrahymena thermophila bF5 by Microcalorimetry

The toxic effect of solubilizing excipients on Tetrahymena thermophila BF5 growth investigated by microcalorimetry

Effects of Artemisinin Derivative on the Growth Metabolism of Tetrahymena thermophila BF5 Based on Expression of Thermokinetics

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