Microcalorimetric investigations of the growth of yeast. Energy balance of batch and continuous cultures.

Brettel, R.; Lamprecht, I.; Schaarschmidt, B.

doi:10.1016/0040-6031(81)85143-x

Cited by 7 publications

(2 citation statements)

References 4 publications

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“…The flux balance framework presented in this review, culminating in Equation 13, provides a quantitative method to measure energetic costs of cellular processes by studying changes in ATP fluxes upon modulation of process activity. The dynamics of global energetic fluxes such as OCR and heat flux have been measured in many biological contexts, including microbial adaptation, organ function, and embryo development (29,30,99,(130)(131)(132)(133)(134)(135)(136)(137)(138)(139)(140)(141). However, the contributions of specific energy-consuming processes such as biosynthesis, ion pumping, and cytoskeletal remodeling to changes in global fluxes remain unclear.…”

Section: Open Questionsmentioning

confidence: 99%

Dissecting Flux Balances to Measure Energetic Costs in Cell Biology: Techniques and Challenges

Arunachalam

Ireland

Yang

et al. 2023

Annu. Rev. Condens. Matter Phys.

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Life is a nonequilibrium phenomenon: Metabolism provides a continuous supply of energy that drives nearly all cellular processes. However, very little is known about how much energy different cellular processes use, i.e., their energetic costs. The most direct experimental measurements of these costs involve modulating the activity of cellular processes and determining the resulting changes in energetic fluxes. In this review, we present a flux balance framework to aid in the design and interpretation of such experiments and discuss the challenges associated with measuring the relevant metabolic fluxes. We then describe selected techniques that enable measurement of these fluxes. Finally, we review prior experimental and theoretical work that has employed techniques from biochemistry and nonequilibrium physics to determine the energetic costs of cellular processes. Expected final online publication date for the Annual Review of Condensed Matter Physics, Volume 14 is March 2023. Please see http://www.annualreviews.org/page/journal/pubdates for revised estimates.

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Section: Open Questionsmentioning

confidence: 99%

Dissecting Flux Balances to Measure Energetic Costs in Cell Biology: Techniques and Challenges

Arunachalam

Ireland

Yang

et al. 2023

Annu. Rev. Condens. Matter Phys.

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show abstract

“…13, provides a quantitative method to measure energetic costs of cellular processes by studying changes in ATP fluxes upon modulation of process activity. The dynamics of global energetic fluxes such as oxygen consumption rate and heat flux have been measured in many biological contexts, including microbial adaptation, organ function, and embryo development [30,31,100,[132][133][134][135][136][137][138][139][140][141][142][143]. However, the contributions of specific energy-consuming processes such as biosynthesis, ion pumping, and cytoskeletal remodeling to changes in global fluxes remain unclear.…”

Section: Open Questionsmentioning

confidence: 99%

Dissecting flux balances to measure energetic costs in cell biology: techniques and challenges

Arunachalam¹,

Ireland²,

Yang³

et al. 2022

Preprint

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Life is a nonequilibrium phenomenon: metabolism provides a continuous supply of energy that drives nearly all cellular processes. However, very little is known about how much energy different cellular processes use, i.e. their energetic costs. The most direct experimental measurements of these costs involve modulating the activity of cellular processes and determining the resulting changes in energetic fluxes. In this review, we present a flux balance framework to aid in the design and interpretation of such experiments, and discuss the challenges associated with measuring the relevant metabolic fluxes. We then describe selected techniques that enable measurement of these fluxes. Finally, we review prior experimental and theoretical work that has employed techniques from biochemistry and nonequilibrium physics to determine the energetic costs of cellular processes.

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Microcalorimetric studies of the mode of action of environmental chemicals on continuous microbial cultures

Weppen

Schüller

1984

Thermochimica Acta

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Microcalorimetric investigations of the growth of yeast. Energy balance of batch and continuous cultures.

Cited by 7 publications

References 4 publications

Dissecting Flux Balances to Measure Energetic Costs in Cell Biology: Techniques and Challenges

Dissecting Flux Balances to Measure Energetic Costs in Cell Biology: Techniques and Challenges

Dissecting flux balances to measure energetic costs in cell biology: techniques and challenges

Microcalorimetric studies of the mode of action of environmental chemicals on continuous microbial cultures

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