Microcalorimetric studies of the mode of action of environmental chemicals on continuous microbial cultures

Weppen, Peter; Schüller, D.

doi:10.1016/0040-6031(84)85059-5

Cited by 23 publications

(4 citation statements)

References 8 publications

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“…However, at present there are few, if any, other methods that possess such general validity as microcalorimetry for analyzing metabolic activities. [22] Most metabolic events in any living system involve biochemical reactions that produce heat. Microcalorimetry has been used successfully to measure the effects of various substances and culture conditions on metabolism.…”

Section: Introductionmentioning

confidence: 99%

A microcalorimetric method for studying the toxic effect of different diphenol species on the growth ofEscherichia coli

Chen

Yao

Wang

et al. 2007

Journal of Environmental Science and Health, Part A

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A microcalorimetric technique based on bacterial heat-output was explored to evaluate the toxic effect of different diphenol species on the growth of Escherichia coli (E. coli). Power-time curves of the growth metabolism for E. coli in the presence of different diphenol species were studied using a multi-channel microcalorimetric system with an ampoule method at 37 degrees C. The growth rate constant (k), generation time (t(G)), inhibitory ratio (I), half-inhibitory concentration (IC(50)) and the total thermal effect (Q(T)) for E. coli were obtained. The results show that catechol and hydroquinone are more toxic to E. coli than resorcinol. In all cases, the growth rate constants of E. coli (in log phase) decreased as the concentrations of these diphenols increased. Among these diphenols species, catechol was found to be the most poisonous species at an IC(50) of 323.5 micro g/mL against E. coli. Hydroquinone exhibited moderate virulence with an IC(50) of 1196 micro g/mL and resorcinol had the lowest toxicity with an IC(50) of 2113 micro g/mL. The microcalorimetric bioassay can be a quantitative, inexpensive, and versatile method for acute cellular toxicity study.

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Section: Introductionmentioning

confidence: 99%

A microcalorimetric method for studying the toxic effect of different diphenol species on the growth ofEscherichia coli

Chen

Yao

Wang

et al. 2007

Journal of Environmental Science and Health, Part A

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“…2) increased heat production was observed. Increased heat production at lower concentrations of phenol, 2‐nitrophenol and 2,4‐nitrophenol added to E. coli (Weppen and Schuller, 1984; Weppen, 1985), PCP on soil microflora and insects (Drong, 1991), 2,4‐D on soil microflora (Prado and Airoldi, 2000) and as answer to potassium stress on amphipods (Koop et al, 1995) was also found. The latter authors showed that the increase originated from the change in metabolism.…”

Section: Discussionmentioning

confidence: 93%

“…The effect of chemicals, such as organic components (Garedew et al, 2002; Critter et al, 2003a; Laor et al, 2004; Li‐Xia et al, 2006; Chen et al, 2009a, b), including biocides (Ljungholm et al, 1979a; Weppen and Schuller, 1984; Weppen, 1985; Drong et al, 1991; Tancho et al, 1992; von Rege and Sand, 1998; Prado and Airoldi, 2000, 2002); metals (Airoldi and Critter, 1996; Zheng et al, 2006; Wang et al, 2009; Zhou et al, 2009) and other inorganic components (Ljungholm et al, 1979b; Xi et al, 2002; Ruming et al, 2006; Chen et al, 2005) on test organisms, microbial communities and soil's own microflora has also been examined by microcalorimetry in some cases. The listed experiments were mainly conducted in aquatic solutions and soil extracts, but some used tests, which allowed the direct interaction of polluted soils and test organisms.…”

Section: Introductionmentioning

confidence: 99%

Environmental toxicity testing of contaminated soil based on microcalorimetry

Gruiz

Feigl

Hajdu

et al. 2010

Environmental Toxicology

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Contaminated site assessment and monitoring requires efficient risk-management tools including innovative environmental toxicity tests. The first application of microcalorimetry for toxicity testing draw the attention to a possible new tool to increase sensitivity, to eliminate matrix effect and to study effect-mechanism. A Thermal Activity Monitor (TAM) microcalorimeter was used for measuring the heat production of various test organisms when getting in contact with sterile toxic soils. Well known bacterial (Azomonas agilis), animal (Folsomia candida) and plant test organisms (Sinapis alba) were tested for heat production. The heat response of selected testorganisms was measured in case of metal (Cu and Zn) and organic pollutant (Diesel oil, DBNPA and PCP) contaminated soils. In addition to the quantitative determination of the heat production, the mechanism of the toxic effect can be characterized from the shape of the power-time curve (slope of the curve, height and time of the maximum). In certain concentration ranges the higher the pollutant concentration of the soil the lower the maximum of the time-heat curve. At low pollutant concentrations an increased heat production was measured in case of A. agile and 20 and 200 mg Zn kg(-1) soil. The microcalorimetric testing was more sensitive in all cases than the traditional test methods. Our results showed that the microcalorimetric test method offers a new and sensitive option in environmental toxicology, both for research and routine testing.

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“…[25]. In this study, the microcalorimetric method was successfully used to analyze the inhibitory effect of palmatine on T. thermophila BF 5 growth.…”

Section: Discussionmentioning

confidence: 99%

Action of palmatine on Tetrahymena thermophila BF5 growth investigated by microcalorimetry

Kong

Yan²,

Xiao³

et al. 2009

Journal of Hazardous Materials

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Microcalorimetric studies of the mode of action of environmental chemicals on continuous microbial cultures

Cited by 23 publications

References 8 publications

A microcalorimetric method for studying the toxic effect of different diphenol species on the growth ofEscherichia coli

A microcalorimetric method for studying the toxic effect of different diphenol species on the growth ofEscherichia coli

Environmental toxicity testing of contaminated soil based on microcalorimetry

Action of palmatine on Tetrahymena thermophila BF5 growth investigated by microcalorimetry

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