Microcapsules with improved mechanical stability for hepatocyte culture

Yin, Chao; Chia, Ser Mien; Quek, Chai Hoon; Yu, Hanry; Zhuo, Ren‐Xi; Leong, Kam W.; Mao, Hai‐Quan

doi:10.1016/s0142-9612(02)00580-x

Cited by 65 publications

(28 citation statements)

References 14 publications

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“…C-RICM in conjunction with phase contrast microscopy has been recently applied to study cell adhesion dynamics [14], liposome-chitosan interaction [15] and colloidal adhesion [16]. The visualization of contact zone formed by a polymeric microparticle on a rigid substrate is the prerequisite for measuring adhesion energy.…”

Section: Resultsmentioning

confidence: 99%

Bioadhesive characterization of poly(methylidene malonate 2.12) microparticle on model extracellular matrix

et al. 2004

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Section: Resultsmentioning

confidence: 99%

Bioadhesive characterization of poly(methylidene malonate 2.12) microparticle on model extracellular matrix

et al. 2004

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“…This is echoed by a number of studies aiming to immobilize hepatocytes in porous scaffolds or on substrates through surface conjugation of functional ligands (e.g. galactosylation [9,10,32,33], collagen treatment [9,10,26,30,31]) or high-density cell entrapment [15,16,38].…”

Section: Discussionmentioning

confidence: 99%

“…Hepatocytes were harvested from male Wister rats weighting from 250 to 300 g by a two-step in situ collagenase perfusion as described previously [26]. Rats were maintained on ad libitum rodent feed and water at room temperature and 40% humidity.…”

Section: Hepatocytes Isolationmentioning

confidence: 99%

Stable immobilization of rat hepatocyte spheroids on galactosylated nanofiber scaffold

Chua

Lim²,

Zhang³

et al. 2005

Biomaterials

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256

160

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“…It has been shown that immunoprotected cells such as pancreatic islets or hepatocytes can respond physiologically both in vitro and in vivo in response to appropriate stimuli [5][6][7].…”

Section: Introductionmentioning

confidence: 99%

Biocompatibility of nanoporous alumina membranes for immunoisolation

Flamme¹,

Popat²,

Leoni³

et al. 2007

Biomaterials

160

108

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Cellular immunoisolation using semi-permeable barriers has been investigated over the past several decades as a promising treatment approach for diseases such as Parkinson's, Alzheimer's, and Type 1 diabetes. Typically, polymeric membranes are used for immunoisolation applications; however, recent advances in technology have led to the development of more robust membranes that are able to more completely meet the requirements for a successful immunoisolation device, including well controlled pore size, chemical and mechanical stability, non-biodegradability, and biocompatibility with both the graft tissue as well as the host. It has been shown previously that nanoporous alumina biocapsules can act effectively as immunoisolation devices, and support the viability and functionality of encapsulated β cells. The aim of this investigation was to assess the biocompatibility of the material with host tissue. The cytotoxicity of the capsule, as well as its ability to activate complement and inflammation was studied. Further, the effects of PEGmodification on the tissue response to implanted capsules were studied. Our results have shown that the device is non-toxic and does not induce significant complement activation. Further, in vivo work has demonstrated that implantation of these capsules into the peritoneal cavity of rats induces a transient inflammatory response, and that PEG is useful in minimizing the host response to the material.

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Microcapsules with improved mechanical stability for hepatocyte culture

Cited by 65 publications

References 14 publications

Bioadhesive characterization of poly(methylidene malonate 2.12) microparticle on model extracellular matrix

Bioadhesive characterization of poly(methylidene malonate 2.12) microparticle on model extracellular matrix

Stable immobilization of rat hepatocyte spheroids on galactosylated nanofiber scaffold

Biocompatibility of nanoporous alumina membranes for immunoisolation

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