Microchamber arrays for the identification of individual cells exposed to an X-ray microbeam

Abstract

To identify individual cells exposed to a X-ray microbeam in a cell population, we developed a biocompatible microchamber-array chip using UV lithography of photopolymer SU-8. The center-to-center distance between microchambers is 50 lm including a wall of 15 lm height. Using the microchamber-array chip, we performed tracking of individual exposed cells. Sample cells loaded in a microchamber array were selectively irradiated with the Xray microbeam under microscopic observation. All the irradiated cells were indexed by the array arrangement of the microchambers. For about 24 h of post-irradiation incubation, the irradiated cells were identified successfully by time-lapse observation. In addition, the induction of radiation effects was observed in identified cells using immunofluorescence.

Applications of SU-8 in the development of a Single Ion Hit Facility

“…SU-8 was chosen previously as a seat layer at the LNM to fabricate custom Petri dishes suitable for cell incubation and irradiation [8]. A similar design has been used for time-tracking living cells after X-rays irradiation [9]. Since the fabrication of the artificial cell sample requires a cell friendly material, SU-8 was chosen once more.…”

Polyallylamine as an Adhesion Promoter for SU-8 Photoresist

“…1) has found widespread use in the microelectronics industry (Djakov et al, 2014), as well as in replica molding, as a mask for metallization on organic substrates, and, due to its transparency, as a structural material for optics (Balslev et al, 2006). SU-8 is a negative photoresist, and favored for HAR lithography, due to its high chemical and mechanical stability, wide variety of thicknesses attainable, and biocompatibility (Gutierrez-Rivera & Cescato, 2008; Kuchimaru et al, 2008; Kwon et al, 2009). SU-8 epoxy resin is developed at near-ultraviolet (UV) wavelengths where the photoresist has very low optical absorption, usually from 365–436 nm, which makes HAR photolithography of thick films possible.…”

Development of Cell Chip Based on Track Detector for Examination of Biological Damage by Alpha Particles

“…The SU-8 is suitable for the production of a structure with a high aspect ratio by solidification through the exposure to UV light. It is widely used as a photofabrication material, which has good characteristics of optical transparency, manufacturability, biocompatibility, 11) and others. The CR-39 films were spin-coated with SU-8 photoresist (SU-8 3005, Kayaku Microchem).…”