Microchannel electrophoretic separation of biogenic amines by micellar electrokinetic chromatography

Rodríguez, Isabel; Lee, Hian Kee; Li, Sam Fong Yau

doi:10.1002/(sici)1522-2683(19990101)20:1<118::aid-elps118>3.0.co;2-2

Cited by 37 publications

(15 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…For example, Arce and co-workers [40] demonstrated the electromigrated separation of 21 amino compounds containing biogenic amines by CZE with indirect UV detection. Nevertheless, recently a more efficient separation of biogenic amines has been accomplished by chip-based MEKC (with analyte derivatisation) as reported by Li et al [41]. They demonstrated that eight biogenic amines could be separated by microchannel MEKC using sodium dodecyl sulphate (SDS) as the surfactant.…”

Section: Introductionmentioning

confidence: 99%

A polydimethylsiloxane/glass capillary electrophoresis microchip for the analysis of biogenic amines using indirect fluorescence detection

Beard

Mello

2002

Electrophoresis

View full text Add to dashboard Cite

A polydimethylsiloxane-glass capillary microchip is fabricated for the rapid analysis of a mixture of common biogenic amines using indirect fluorescence detection. Using a running buffer of phosphate and 2-propanol, and Rhodamine 110 as a background fluorophore, both co-ionic and counter-ionic systems are explored. Studies demonstrate the separation and analysis of cations using indirect fluorescence detection for the first time in a chip-based system. Resulting electrophoretic separations are achieved within a few tens of seconds with detection limits of approximately 6 microM. The reduced sample handling and rapid separations afforded by the coupling of indirect fluorescence detection with chip-based capillary electrophoresis provide a highly efficient method for the analysis and detection of molecules not possessing a chromophore or fluorophore. Furthermore, limits of detection are on a par with reported chip-based protocols that incorporate precolumn derivatisation with fluorescence detection. The current device circumvents lengthy sample preparation stages and therefore provides an attractive alternative technique for the analysis biogenic amines.

show abstract

Section: Introductionmentioning

confidence: 99%

A polydimethylsiloxane/glass capillary electrophoresis microchip for the analysis of biogenic amines using indirect fluorescence detection

Beard

Mello

2002

Electrophoresis

View full text Add to dashboard Cite

show abstract

“…The analysis time is about 80 s, the run-to-run reproducibility of migration times varies between 0.15 and 0.54%, day-to-day reproducibility of migration times is between 0.40 and 0.80%, and the detection limit varies between 2.94 mM (putrescine) and 6.57 mM (spermine). The method was applied to real samples by analyzing biogenic amines in soy sauce [130].…”

Section: Other Applicationsmentioning

confidence: 99%

Capillary electrophoresis on microchip

Dolnı́k¹,

Liu²,

Jovanovich³

2000

Electrophoresis

480

331

View full text Add to dashboard Cite

Capillary electrophoresis and related techniques on microchips have made great strides in recent years. This review concentrates on progress in capillary zone electrophoresis, but also covers other capillary techniques such as isoelectric focusing, isotachophoresis, free flow electrophoresis, and micellar electrokinetic chromatography. The material and technologies used to prepare microchips, microchip designs, channel geometries, sample manipulation and derivatization, detection, and applications of capillary electrophoresis to microchips are discussed. The progress in separation of nucleic acids and proteins is particularly emphasized.

show abstract

“…Various detection methods are used for on-chip detection, principally fluorescence (Colyer et al, 1997a,b; Hadd et al, 1997), but other techniques such as chemiluminescence (Mangru and Harrison, 1998) and electrochemical detection (Wooley et al, 1998) are also effective. A range of analytical techniques have been adapted to a microformat including gas chromatography (Bruns, 1994), micellar electrokinetic chromatography (Rodriguez et al, 1999) isoelectric focussing (Wen et al, 2000), and isotachophoresis (Walker et al, 1998). Miniaturization of capillary electrophoresis is currently one of the most successful microchip applications (Colyer et al, 1997a,b; Dolnik et al, 2000; Hashimoto et al, 2000; Hofgartner et al, 1999; Huang et al, 1999; Liu et al, 1999; Munro et al, 1999; Rodriguez et al, 1997a,b; Rossier et al, 1999; Schultz-Lockyear et al, 1999; Ueda et al, 2000) and an analyzer is now available commercially (www.agilent.com).…”

Section: Microanalytical Devicesmentioning

confidence: 99%

Microchips, microarrays, biochips and nanochips: personal laboratories for the 21st century

Kricka

2001

Clinica Chimica Acta

174

View full text Add to dashboard Cite

Microchannel electrophoretic separation of biogenic amines by micellar electrokinetic chromatography

Cited by 37 publications

References 35 publications

A polydimethylsiloxane/glass capillary electrophoresis microchip for the analysis of biogenic amines using indirect fluorescence detection

A polydimethylsiloxane/glass capillary electrophoresis microchip for the analysis of biogenic amines using indirect fluorescence detection

Capillary electrophoresis on microchip

Microchips, microarrays, biochips and nanochips: personal laboratories for the 21st century

Contact Info

Product

Resources

About